Ruminococcus sp. AF41-9

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; unclassified Ruminococcus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3566 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A416B2N0|A0A416B2N0_9FIRM LacI family transcriptional regulator OS=Ruminococcus sp. AF41-9 OX=2292069 GN=DW049_17955 PE=4 SV=1
MM1 pKa = 7.69EE2 pKa = 5.03YY3 pKa = 8.92EE4 pKa = 4.02TALFDD9 pKa = 3.78TDD11 pKa = 4.2SVISVNILMDD21 pKa = 3.95EE22 pKa = 4.48DD23 pKa = 3.83DD24 pKa = 4.23WEE26 pKa = 4.25EE27 pKa = 3.9MLDD30 pKa = 3.59NAISEE35 pKa = 4.83EE36 pKa = 4.05YY37 pKa = 9.98HH38 pKa = 5.89KK39 pKa = 11.37CNVEE43 pKa = 3.91INGKK47 pKa = 6.97TFYY50 pKa = 10.56QVAIRR55 pKa = 11.84PKK57 pKa = 10.27GNTSLSSIASDD68 pKa = 3.42PTTDD72 pKa = 3.06RR73 pKa = 11.84YY74 pKa = 10.97SFKK77 pKa = 11.2LEE79 pKa = 3.44FDD81 pKa = 4.07HH82 pKa = 7.22YY83 pKa = 11.67VDD85 pKa = 3.91GQTCFGLDD93 pKa = 4.13KK94 pKa = 11.21LILNNNYY101 pKa = 10.46ADD103 pKa = 3.7ATNMKK108 pKa = 9.48EE109 pKa = 3.68ALIYY113 pKa = 11.04DD114 pKa = 4.28MYY116 pKa = 11.26QYY118 pKa = 11.37LGADD122 pKa = 3.05ASLYY126 pKa = 10.17NYY128 pKa = 11.09AEE130 pKa = 3.78ISVNGEE136 pKa = 3.49YY137 pKa = 9.85WGVYY141 pKa = 8.99LALEE145 pKa = 4.19AVEE148 pKa = 5.94DD149 pKa = 4.07SFLLRR154 pKa = 11.84NYY156 pKa = 7.36GTQDD160 pKa = 3.0GEE162 pKa = 4.44LYY164 pKa = 10.45KK165 pKa = 10.58PEE167 pKa = 4.17SMDD170 pKa = 3.05MGGAPGGDD178 pKa = 3.05QDD180 pKa = 6.41SNDD183 pKa = 4.21QEE185 pKa = 5.39SKK187 pKa = 10.22DD188 pKa = 3.62QKK190 pKa = 11.32SKK192 pKa = 11.06GKK194 pKa = 10.59APANGNFANMTPPDD208 pKa = 4.38FSDD211 pKa = 4.23SDD213 pKa = 4.13DD214 pKa = 3.89NQGKK218 pKa = 9.83DD219 pKa = 3.31FSGFPEE225 pKa = 4.16RR226 pKa = 11.84NFDD229 pKa = 3.97FDD231 pKa = 4.44NMPDD235 pKa = 3.26MDD237 pKa = 4.91MPDD240 pKa = 3.33MDD242 pKa = 5.16MPDD245 pKa = 3.21MDD247 pKa = 5.26AGGFSMGGNGADD259 pKa = 4.57LNYY262 pKa = 10.65SDD264 pKa = 6.15DD265 pKa = 5.26DD266 pKa = 4.28PDD268 pKa = 4.09SYY270 pKa = 11.1STIWDD275 pKa = 3.75GEE277 pKa = 3.99ITNTKK282 pKa = 9.8KK283 pKa = 10.57ADD285 pKa = 3.54HH286 pKa = 6.48KK287 pKa = 11.08RR288 pKa = 11.84VMTALKK294 pKa = 10.3NISEE298 pKa = 4.35GNDD301 pKa = 3.03LEE303 pKa = 5.05SYY305 pKa = 10.44MDD307 pKa = 3.58IDD309 pKa = 3.55NLLRR313 pKa = 11.84YY314 pKa = 7.74MAVHH318 pKa = 6.51IFSVNEE324 pKa = 3.94DD325 pKa = 3.86SLSGTMAHH333 pKa = 6.62NYY335 pKa = 9.44YY336 pKa = 10.33LYY338 pKa = 10.59EE339 pKa = 4.31SSGEE343 pKa = 4.02LNLIPWDD350 pKa = 3.87YY351 pKa = 11.59NFSEE355 pKa = 4.64NNSQTTVVSSLVLYY369 pKa = 9.82GVSFFILIAALIFALLYY386 pKa = 10.16RR387 pKa = 11.84RR388 pKa = 11.84RR389 pKa = 3.67

Molecular weight: 43.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A416B1V3|A0A416B1V3_9FIRM Uncharacterized protein OS=Ruminococcus sp. AF41-9 OX=2292069 GN=DW049_18100 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.69VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.41GRR39 pKa = 11.84KK40 pKa = 8.72HH41 pKa = 6.34LSAA44 pKa = 5.8

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3566

0

3566

1070409

25

2817

300.2

33.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.207 ± 0.045

1.533 ± 0.019

5.706 ± 0.037

7.792 ± 0.053

4.024 ± 0.028

6.901 ± 0.041

1.772 ± 0.022

7.179 ± 0.04

7.222 ± 0.037

8.761 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.144 ± 0.026

4.471 ± 0.035

3.226 ± 0.024

3.466 ± 0.028

4.309 ± 0.033

5.841 ± 0.037

5.553 ± 0.04

6.725 ± 0.038

0.939 ± 0.014

4.23 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski