Odonata-associated circular virus-19
Average proteome isoelectric point is 8.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4UH55|A0A0B4UH55_9VIRU Putative capsid protein OS=Odonata-associated circular virus-19 OX=1592119 PE=4 SV=1
MM1 pKa = 7.51 SFQYY5 pKa = 10.83 HH6 pKa = 5.54 NFVFTSFKK14 pKa = 10.46 TEE16 pKa = 3.74 DD17 pKa = 3.39 FEE19 pKa = 5.44 RR20 pKa = 11.84 VLGLDD25 pKa = 3.05 VQYY28 pKa = 10.79 IIVGKK33 pKa = 7.05 EE34 pKa = 3.69 TCPEE38 pKa = 4.03 TKK40 pKa = 9.58 RR41 pKa = 11.84 EE42 pKa = 3.95 HH43 pKa = 5.72 LQGYY47 pKa = 9.45 CEE49 pKa = 4.44 LDD51 pKa = 3.24 KK52 pKa = 11.07 KK53 pKa = 10.22 KK54 pKa = 9.44 TMAGIKK60 pKa = 10.11 KK61 pKa = 10.01 LFSDD65 pKa = 4.42 DD66 pKa = 3.79 SLHH69 pKa = 7.05 IEE71 pKa = 4.32 ARR73 pKa = 11.84 KK74 pKa = 8.35 GTQKK78 pKa = 10.63 EE79 pKa = 4.05 AIIYY83 pKa = 7.25 CQKK86 pKa = 9.89 EE87 pKa = 4.6 GNWKK91 pKa = 9.3 EE92 pKa = 4.02 AGVQRR97 pKa = 11.84 KK98 pKa = 8.55 QGSRR102 pKa = 11.84 GDD104 pKa = 3.67 LDD106 pKa = 3.81 GARR109 pKa = 11.84 VMAVEE114 pKa = 4.05 SGMRR118 pKa = 11.84 AVSRR122 pKa = 11.84 SMNMQQIRR130 pKa = 11.84 VAEE133 pKa = 4.13 KK134 pKa = 8.39 FLSYY138 pKa = 10.96 NEE140 pKa = 4.47 EE141 pKa = 3.79 VRR143 pKa = 11.84 DD144 pKa = 3.86 WKK146 pKa = 10.92 PEE148 pKa = 3.87 VLWFWGDD155 pKa = 3.29 SGSGKK160 pKa = 9.92 SRR162 pKa = 11.84 EE163 pKa = 4.0 ARR165 pKa = 11.84 FISHH169 pKa = 7.07 GLDD172 pKa = 2.87 TYY174 pKa = 11.43 VKK176 pKa = 9.09 NTGGKK181 pKa = 7.54 WWDD184 pKa = 3.89 GYY186 pKa = 11.03 DD187 pKa = 3.25 GHH189 pKa = 6.44 EE190 pKa = 4.31 VVIIDD195 pKa = 5.29 DD196 pKa = 5.29 FRR198 pKa = 11.84 DD199 pKa = 3.46 SWWALTYY206 pKa = 10.22 FLACTDD212 pKa = 3.24 RR213 pKa = 11.84 YY214 pKa = 10.09 EE215 pKa = 4.25 FQVEE219 pKa = 4.32 VKK221 pKa = 10.7 GGFRR225 pKa = 11.84 QLRR228 pKa = 11.84 AKK230 pKa = 9.85 MIIITSLFHH239 pKa = 6.09 PKK241 pKa = 10.22 YY242 pKa = 9.49 MYY244 pKa = 10.58 RR245 pKa = 11.84 EE246 pKa = 4.1 AQAKK250 pKa = 10.16 GDD252 pKa = 3.49 TAYY255 pKa = 10.89 QILRR259 pKa = 11.84 RR260 pKa = 11.84 IDD262 pKa = 3.69 SVVHH266 pKa = 6.3 FGSQKK271 pKa = 10.6 PEE273 pKa = 3.72 VGGNTIAPTVSNFIDD288 pKa = 3.61 EE289 pKa = 4.91 TIGLLL294 pKa = 4.02
Molecular weight: 33.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.73
IPC2_protein 6.942
IPC_protein 7.088
Toseland 6.985
ProMoST 7.512
Dawson 7.702
Bjellqvist 7.658
Wikipedia 7.644
Rodwell 7.702
Grimsley 7.073
Solomon 7.819
Lehninger 7.834
Nozaki 7.936
DTASelect 7.819
Thurlkill 7.878
EMBOSS 7.98
Sillero 8.097
Patrickios 4.457
IPC_peptide 7.819
IPC2_peptide 7.132
IPC2.peptide.svr19 7.306
Protein with the highest isoelectric point:
>tr|A0A0B4UH55|A0A0B4UH55_9VIRU Putative capsid protein OS=Odonata-associated circular virus-19 OX=1592119 PE=4 SV=1
MM1 pKa = 7.59 AIIRR5 pKa = 11.84 RR6 pKa = 11.84 SFRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 PSARR16 pKa = 11.84 KK17 pKa = 5.41 PTRR20 pKa = 11.84 RR21 pKa = 11.84 YY22 pKa = 6.9 TRR24 pKa = 11.84 RR25 pKa = 11.84 PRR27 pKa = 11.84 VAAKK31 pKa = 9.0 RR32 pKa = 11.84 TGAPRR37 pKa = 11.84 TMRR40 pKa = 11.84 NSNTSQLIAPRR51 pKa = 11.84 YY52 pKa = 6.86 ITTLKK57 pKa = 9.51 YY58 pKa = 10.3 CEE60 pKa = 4.33 RR61 pKa = 11.84 DD62 pKa = 3.61 SVVTSVSGASGADD75 pKa = 3.44 LYY77 pKa = 11.48 NLNSIFDD84 pKa = 4.27 PDD86 pKa = 3.42 RR87 pKa = 11.84 TGTGHH92 pKa = 6.35 QPLGHH97 pKa = 6.94 DD98 pKa = 3.89 QLQLLYY104 pKa = 10.83 NRR106 pKa = 11.84 YY107 pKa = 9.33 RR108 pKa = 11.84 VFGVSWRR115 pKa = 11.84 VTFYY119 pKa = 11.19 QPGAGVAGLFAVFPQNNALTQNGVSTSVLKK149 pKa = 10.19 EE150 pKa = 3.84 LPHH153 pKa = 7.0 AFTKK157 pKa = 10.74 LSRR160 pKa = 11.84 ADD162 pKa = 3.47 QPVVFTGRR170 pKa = 11.84 VSLPKK175 pKa = 9.92 LTGQTPAQFKK185 pKa = 10.95 AGLEE189 pKa = 4.06 YY190 pKa = 10.6 QATFGASPSEE200 pKa = 4.0 LQTLLVAVNPVTTGAQTFNYY220 pKa = 9.53 EE221 pKa = 3.83 IEE223 pKa = 4.28 LKK225 pKa = 10.83 YY226 pKa = 10.43 HH227 pKa = 5.82 VEE229 pKa = 4.17 CFDD232 pKa = 5.91 PIPLAQSS239 pKa = 3.18
Molecular weight: 26.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.882
IPC_protein 10.76
Toseland 10.526
ProMoST 10.35
Dawson 10.701
Bjellqvist 10.467
Wikipedia 10.95
Rodwell 10.833
Grimsley 10.789
Solomon 10.789
Lehninger 10.745
Nozaki 10.511
DTASelect 10.467
Thurlkill 10.57
EMBOSS 10.935
Sillero 10.628
Patrickios 10.467
IPC_peptide 10.789
IPC2_peptide 9.531
IPC2.peptide.svr19 8.339
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
533
239
294
266.5
30.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.942 ± 1.242
1.126 ± 0.194
4.878 ± 1.312
5.441 ± 1.691
5.253 ± 0.156
7.88 ± 0.798
2.064 ± 0.263
4.69 ± 1.186
5.816 ± 1.662
7.129 ± 1.116
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.876 ± 0.7
3.189 ± 0.67
4.128 ± 2.01
5.253 ± 0.407
7.88 ± 1.174
6.567 ± 0.368
6.942 ± 1.524
7.129 ± 0.271
1.689 ± 0.855
4.128 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here