Pseudomonas phage MR18

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Krylovirinae; unclassified Krylovirinae

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3TE53|A0A6M3TE53_9CAUD Putative DNA endonuclease OS=Pseudomonas phage MR18 OX=2711181 GN=PssvBMR18_gp21 PE=4 SV=1
MM1 pKa = 7.46NSYY4 pKa = 9.54TMTALWIIGCGVAMWALDD22 pKa = 3.7YY23 pKa = 11.4YY24 pKa = 8.42VTSWACIPNN33 pKa = 3.58

Molecular weight: 3.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3TED2|A0A6M3TED2_9CAUD Uncharacterized protein OS=Pseudomonas phage MR18 OX=2711181 GN=PssvBMR18_gp48 PE=4 SV=1
MM1 pKa = 6.33TTIVYY6 pKa = 10.09HH7 pKa = 6.91RR8 pKa = 11.84GTFAWDD14 pKa = 3.31SRR16 pKa = 11.84ITAGNHH22 pKa = 3.96IVTDD26 pKa = 3.31KK27 pKa = 10.77GMKK30 pKa = 9.59RR31 pKa = 11.84FVEE34 pKa = 4.81GGHH37 pKa = 4.2VFYY40 pKa = 10.86RR41 pKa = 11.84RR42 pKa = 11.84NHH44 pKa = 5.38PRR46 pKa = 11.84GG47 pKa = 3.3

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

13441

31

1012

235.8

25.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.885 ± 0.425

1.257 ± 0.194

5.565 ± 0.229

5.193 ± 0.298

3.013 ± 0.177

8.028 ± 0.318

2.053 ± 0.205

4.077 ± 0.166

4.36 ± 0.246

8.928 ± 0.232

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.902 ± 0.153

3.98 ± 0.285

4.293 ± 0.248

4.598 ± 0.326

6.294 ± 0.29

6.13 ± 0.271

6.071 ± 0.289

7.566 ± 0.388

1.555 ± 0.14

3.251 ± 0.203
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski