Pseudomonas phage MR18
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M3TE53|A0A6M3TE53_9CAUD Putative DNA endonuclease OS=Pseudomonas phage MR18 OX=2711181 GN=PssvBMR18_gp21 PE=4 SV=1
MM1 pKa = 7.46 NSYY4 pKa = 9.54 TMTALWIIGCGVAMWALDD22 pKa = 3.7 YY23 pKa = 11.4 YY24 pKa = 8.42 VTSWACIPNN33 pKa = 3.58
Molecular weight: 3.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.312
IPC2_protein 4.673
IPC_protein 3.376
Toseland 3.389
ProMoST 3.872
Dawson 3.554
Bjellqvist 3.795
Wikipedia 3.77
Rodwell 3.389
Grimsley 3.401
Solomon 3.147
Lehninger 3.096
Nozaki 3.897
DTASelect 3.745
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.605
Patrickios 0.006
IPC_peptide 3.134
IPC2_peptide 3.478
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|A0A6M3TED2|A0A6M3TED2_9CAUD Uncharacterized protein OS=Pseudomonas phage MR18 OX=2711181 GN=PssvBMR18_gp48 PE=4 SV=1
MM1 pKa = 6.33 TTIVYY6 pKa = 10.09 HH7 pKa = 6.91 RR8 pKa = 11.84 GTFAWDD14 pKa = 3.31 SRR16 pKa = 11.84 ITAGNHH22 pKa = 3.96 IVTDD26 pKa = 3.31 KK27 pKa = 10.77 GMKK30 pKa = 9.59 RR31 pKa = 11.84 FVEE34 pKa = 4.81 GGHH37 pKa = 4.2 VFYY40 pKa = 10.86 RR41 pKa = 11.84 RR42 pKa = 11.84 NHH44 pKa = 5.38 PRR46 pKa = 11.84 GG47 pKa = 3.3
Molecular weight: 5.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.308
IPC2_protein 9.999
IPC_protein 11.038
Toseland 10.994
ProMoST 11.067
Dawson 11.067
Bjellqvist 10.906
Wikipedia 11.389
Rodwell 11.067
Grimsley 11.125
Solomon 11.33
Lehninger 11.272
Nozaki 10.965
DTASelect 10.906
Thurlkill 10.994
EMBOSS 11.433
Sillero 11.023
Patrickios 10.906
IPC_peptide 11.33
IPC2_peptide 10.101
IPC2.peptide.svr19 8.401
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
57
0
57
13441
31
1012
235.8
25.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.885 ± 0.425
1.257 ± 0.194
5.565 ± 0.229
5.193 ± 0.298
3.013 ± 0.177
8.028 ± 0.318
2.053 ± 0.205
4.077 ± 0.166
4.36 ± 0.246
8.928 ± 0.232
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.902 ± 0.153
3.98 ± 0.285
4.293 ± 0.248
4.598 ± 0.326
6.294 ± 0.29
6.13 ± 0.271
6.071 ± 0.289
7.566 ± 0.388
1.555 ± 0.14
3.251 ± 0.203
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here