halophilic archaeon J07HB67

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; unclassified Halobacteriales

Average proteome isoelectric point is 5.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2831 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U1QX13|U1QX13_9EURY Preprotein translocase subunit SecD OS=halophilic archaeon J07HB67 OX=1085029 GN=J07HB67_00889 PE=4 SV=1
LL1 pKa = 6.63TPAEE5 pKa = 4.06VDD7 pKa = 3.3TPEE10 pKa = 4.7FVLLTHH16 pKa = 6.23GHH18 pKa = 7.0ADD20 pKa = 3.99HH21 pKa = 7.05IAHH24 pKa = 7.04AEE26 pKa = 4.02AFGDD30 pKa = 3.73ATLVATPEE38 pKa = 3.84IVGYY42 pKa = 11.0AEE44 pKa = 4.66AEE46 pKa = 4.11LGFDD50 pKa = 3.9DD51 pKa = 4.55TVGMNLGGTVACGDD65 pKa = 3.75AFVTIHH71 pKa = 7.04RR72 pKa = 11.84ADD74 pKa = 3.42HH75 pKa = 5.99TNGLNTGYY83 pKa = 9.1EE84 pKa = 4.34HH85 pKa = 6.84EE86 pKa = 4.57VGMPAGFVISEE97 pKa = 4.34DD98 pKa = 3.81RR99 pKa = 11.84PTLSGDD105 pKa = 3.47DD106 pKa = 3.66AGTTFYY112 pKa = 10.87HH113 pKa = 6.98AGDD116 pKa = 3.75TGLMTEE122 pKa = 4.0MRR124 pKa = 11.84DD125 pKa = 3.63VIGEE129 pKa = 4.06YY130 pKa = 10.71LEE132 pKa = 4.62PDD134 pKa = 3.52AAAVPAGDD142 pKa = 4.61HH143 pKa = 5.57FTMGPTQAAIAVDD156 pKa = 3.88WLDD159 pKa = 3.34VDD161 pKa = 4.28HH162 pKa = 7.44AFPMHH167 pKa = 7.07YY168 pKa = 8.84DD169 pKa = 3.67TFPPVEE175 pKa = 4.32IDD177 pKa = 2.9TDD179 pKa = 3.87HH180 pKa = 6.66FVEE183 pKa = 4.89EE184 pKa = 4.44VAATGSDD191 pKa = 3.09ATVEE195 pKa = 4.09VLDD198 pKa = 4.16GDD200 pKa = 3.87EE201 pKa = 4.41TFTLEE206 pKa = 4.13

Molecular weight: 21.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U1PMN8|U1PMN8_9EURY Sortase related acyltransferase OS=halophilic archaeon J07HB67 OX=1085029 GN=J07HB67_00391 PE=4 SV=1
MM1 pKa = 7.45SARR4 pKa = 11.84PGHH7 pKa = 6.57RR8 pKa = 11.84SPTSRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84STRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84APPRR25 pKa = 11.84TRR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84SAHH32 pKa = 4.68PHH34 pKa = 5.58PRR36 pKa = 11.84SSAGRR41 pKa = 11.84SRR43 pKa = 11.84GRR45 pKa = 11.84PTPQSLPGVPSARR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84PSRR66 pKa = 11.84PRR68 pKa = 11.84LGAGHH73 pKa = 6.57GRR75 pKa = 11.84CRR77 pKa = 11.84LPPGSRR83 pKa = 11.84RR84 pKa = 11.84RR85 pKa = 11.84SSRR88 pKa = 11.84RR89 pKa = 11.84HH90 pKa = 5.45PRR92 pKa = 11.84THH94 pKa = 6.58EE95 pKa = 4.2PPTGSAHH102 pKa = 6.23GSQRR106 pKa = 11.84RR107 pKa = 11.84HH108 pKa = 5.72APAPPDD114 pKa = 3.73DD115 pKa = 4.2PPRR118 pKa = 11.84GGGCGARR125 pKa = 11.84PRR127 pKa = 11.84RR128 pKa = 11.84APVGGRR134 pKa = 11.84PRR136 pKa = 11.84GPCRR140 pKa = 11.84HH141 pKa = 6.34RR142 pKa = 11.84PPPRR146 pKa = 11.84PTRR149 pKa = 11.84RR150 pKa = 11.84GSRR153 pKa = 11.84RR154 pKa = 11.84RR155 pKa = 11.84GRR157 pKa = 11.84SRR159 pKa = 11.84STRR162 pKa = 11.84RR163 pKa = 11.84YY164 pKa = 8.77GPVRR168 pKa = 11.84VAGWRR173 pKa = 11.84ACRR176 pKa = 11.84RR177 pKa = 11.84RR178 pKa = 11.84RR179 pKa = 11.84RR180 pKa = 11.84RR181 pKa = 11.84RR182 pKa = 3.18

Molecular weight: 20.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2831

0

2831

650011

23

1302

229.6

24.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.517 ± 0.063

0.738 ± 0.014

8.302 ± 0.056

7.756 ± 0.061

3.059 ± 0.034

8.977 ± 0.062

1.902 ± 0.023

2.802 ± 0.033

1.276 ± 0.021

8.517 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.435 ± 0.023

1.958 ± 0.029

5.037 ± 0.037

2.385 ± 0.025

7.701 ± 0.057

5.405 ± 0.049

7.467 ± 0.049

10.092 ± 0.064

1.06 ± 0.016

2.359 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski