Meiothermus phage MMP17
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A411CW21|A0A411CW21_9CAUD Uncharacterized protein OS=Meiothermus phage MMP17 OX=2483662 PE=4 SV=1
MM1 pKa = 7.24 CQEE4 pKa = 4.39 CQGCGYY10 pKa = 10.11 VEE12 pKa = 4.32 YY13 pKa = 10.92 VAGFYY18 pKa = 10.83 FSDD21 pKa = 3.39 SFGNYY26 pKa = 9.42 LPRR29 pKa = 11.84 EE30 pKa = 4.13 EE31 pKa = 4.13 IARR34 pKa = 11.84 CEE36 pKa = 4.28 VCDD39 pKa = 3.43 GTGFVEE45 pKa = 5.14 VWDD48 pKa = 3.73 EE49 pKa = 4.3 DD50 pKa = 4.06 EE51 pKa = 5.73 AVLEE55 pKa = 4.42 GDD57 pKa = 3.85 GG58 pKa = 4.34
Molecular weight: 6.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.007
IPC2_protein 3.592
IPC_protein 3.439
Toseland 3.287
ProMoST 3.63
Dawson 3.439
Bjellqvist 3.605
Wikipedia 3.376
Rodwell 3.3
Grimsley 3.21
Solomon 3.376
Lehninger 3.325
Nozaki 3.592
DTASelect 3.668
Thurlkill 3.363
EMBOSS 3.389
Sillero 3.567
Patrickios 0.006
IPC_peptide 3.376
IPC2_peptide 3.528
IPC2.peptide.svr19 3.628
Protein with the highest isoelectric point:
>tr|A0A411CVZ3|A0A411CVZ3_9CAUD Uncharacterized protein OS=Meiothermus phage MMP17 OX=2483662 PE=4 SV=1
MM1 pKa = 7.55 GWRR4 pKa = 11.84 SIPSWGSRR12 pKa = 11.84 SFSRR16 pKa = 11.84 KK17 pKa = 9.44 SPAHH21 pKa = 5.98 RR22 pKa = 11.84 AGGGATSLRR31 pKa = 11.84 RR32 pKa = 11.84 AWPSGSSATAKK43 pKa = 9.4 WGCLSRR49 pKa = 11.84 SSSTRR54 pKa = 11.84 RR55 pKa = 11.84 GSAGGLRR62 pKa = 11.84 GMTATTPATPHH73 pKa = 5.7 MRR75 pKa = 11.84 CPAPPAPARR84 pKa = 11.84 GVGATTSPVGGARR97 pKa = 11.84 GGEE100 pKa = 3.83 PMARR104 pKa = 11.84 RR105 pKa = 11.84 VKK107 pKa = 10.48 QIAPGLSVVKK117 pKa = 8.67 TAPFWRR123 pKa = 11.84 GDD125 pKa = 3.28 DD126 pKa = 3.15 RR127 pKa = 11.84 VYY129 pKa = 11.31 EE130 pKa = 5.0 FIHH133 pKa = 6.5 EE134 pKa = 4.03 PTGAVLCCGSPPLWRR149 pKa = 11.84 FKK151 pKa = 10.92 RR152 pKa = 11.84 RR153 pKa = 11.84 VVEE156 pKa = 3.85 LAEE159 pKa = 4.27 PQLALLDD166 pKa = 3.98 WTNLDD171 pKa = 3.98 PDD173 pKa = 4.86 DD174 pKa = 4.56 PLNYY178 pKa = 9.72 PIYY181 pKa = 9.81 RR182 pKa = 11.84 DD183 pKa = 3.11 EE184 pKa = 4.49 FYY186 pKa = 10.83 RR187 pKa = 11.84 IIRR190 pKa = 11.84 EE191 pKa = 3.96 SLEE194 pKa = 3.65 RR195 pKa = 11.84 ARR197 pKa = 11.84 AEE199 pKa = 4.2 EE200 pKa = 4.48 EE201 pKa = 3.86 EE202 pKa = 4.51 TAA204 pKa = 4.48
Molecular weight: 22.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.385
IPC_protein 10.233
Toseland 10.321
ProMoST 10.116
Dawson 10.482
Bjellqvist 10.248
Wikipedia 10.716
Rodwell 10.555
Grimsley 10.555
Solomon 10.599
Lehninger 10.555
Nozaki 10.379
DTASelect 10.233
Thurlkill 10.365
EMBOSS 10.745
Sillero 10.423
Patrickios 10.233
IPC_peptide 10.599
IPC2_peptide 9.531
IPC2.peptide.svr19 8.474
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
66
0
66
12725
40
748
192.8
21.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.827 ± 0.451
1.061 ± 0.19
4.401 ± 0.218
6.413 ± 0.432
2.452 ± 0.195
9.548 ± 0.424
1.917 ± 0.205
3.788 ± 0.279
2.365 ± 0.266
10.625 ± 0.36
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.525 ± 0.099
2.46 ± 0.302
6.703 ± 0.353
3.859 ± 0.213
8.864 ± 0.397
5.336 ± 0.246
5.1 ± 0.35
6.499 ± 0.309
2.475 ± 0.203
2.782 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here