Streptococcus phage Javan405

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AEN7|A0A4D6AEN7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan405 OX=2548151 GN=Javan405_0027 PE=4 SV=1
MM1 pKa = 7.36WEE3 pKa = 3.55IMTRR7 pKa = 11.84TVGDD11 pKa = 3.29RR12 pKa = 11.84HH13 pKa = 5.37YY14 pKa = 10.66VCEE17 pKa = 4.21FLRR20 pKa = 11.84EE21 pKa = 4.16DD22 pKa = 3.43TTDD25 pKa = 3.05PRR27 pKa = 11.84NIDD30 pKa = 3.61GAWIRR35 pKa = 11.84ILTIKK40 pKa = 10.43RR41 pKa = 11.84DD42 pKa = 3.31GEE44 pKa = 4.16YY45 pKa = 9.72IYY47 pKa = 10.36QYY49 pKa = 10.86RR50 pKa = 11.84YY51 pKa = 10.77GNEE54 pKa = 3.93IDD56 pKa = 4.6NMDD59 pKa = 5.1DD60 pKa = 3.19IDD62 pKa = 3.95RR63 pKa = 11.84TVCQAVLDD71 pKa = 4.16NFNEE75 pKa = 4.18LL76 pKa = 3.58

Molecular weight: 9.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A9Y1|A0A4D6A9Y1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan405 OX=2548151 GN=Javan405_0020 PE=4 SV=1
MM1 pKa = 7.84CKK3 pKa = 10.1RR4 pKa = 11.84GDD6 pKa = 3.1IYY8 pKa = 11.45YY9 pKa = 10.77VNFSEE14 pKa = 6.1LEE16 pKa = 4.01GSHH19 pKa = 5.34YY20 pKa = 9.91QQGRR24 pKa = 11.84RR25 pKa = 11.84PALIVSNDD33 pKa = 3.12LANLHH38 pKa = 5.79SHH40 pKa = 6.75LVTVVPLTSQVKK52 pKa = 9.72KK53 pKa = 10.57KK54 pKa = 10.64KK55 pKa = 9.61EE56 pKa = 3.96LPTHH60 pKa = 6.05VFLPQGVIKK69 pKa = 10.27GLKK72 pKa = 9.6RR73 pKa = 11.84SSLVLAEE80 pKa = 4.09QVSTIDD86 pKa = 4.81KK87 pKa = 10.9GLLEE91 pKa = 5.2DD92 pKa = 5.8KK93 pKa = 10.43IACVWDD99 pKa = 4.05KK100 pKa = 11.55NWLNKK105 pKa = 9.17IDD107 pKa = 3.87QALKK111 pKa = 10.16IQLGLL116 pKa = 3.59

Molecular weight: 13.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38

0

38

11775

37

1039

309.9

34.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.641 ± 0.413

0.79 ± 0.124

5.996 ± 0.303

7.04 ± 0.333

3.805 ± 0.261

6.837 ± 0.297

2.03 ± 0.149

6.335 ± 0.336

7.159 ± 0.257

9.325 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.234 ± 0.15

4.263 ± 0.255

2.938 ± 0.231

4.467 ± 0.222

4.433 ± 0.293

7.219 ± 0.337

6.565 ± 0.456

6.718 ± 0.244

1.486 ± 0.092

3.72 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski