Salmonella phage SI22
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C0CGZ3|A0A5C0CGZ3_9CAUD Tail protein OS=Salmonella phage SI22 OX=2592209 PE=4 SV=1
MM1 pKa = 7.4 QGDD4 pKa = 4.08 TLDD7 pKa = 4.24 VICARR12 pKa = 11.84 YY13 pKa = 9.04 YY14 pKa = 10.75 GRR16 pKa = 11.84 TEE18 pKa = 4.12 GVVEE22 pKa = 4.42 TVLQANPGLSEE33 pKa = 4.6 LGVILPHH40 pKa = 6.21 GTAIDD45 pKa = 4.16 LPDD48 pKa = 3.87 VPSSPVTEE56 pKa = 4.95 TINLWEE62 pKa = 4.05
Molecular weight: 6.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.814
IPC2_protein 4.05
IPC_protein 3.859
Toseland 3.694
ProMoST 3.961
Dawson 3.846
Bjellqvist 4.126
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.617
Solomon 3.808
Lehninger 3.77
Nozaki 3.986
DTASelect 4.113
Thurlkill 3.757
EMBOSS 3.795
Sillero 3.986
Patrickios 1.901
IPC_peptide 3.821
IPC2_peptide 3.961
IPC2.peptide.svr19 3.941
Protein with the highest isoelectric point:
>tr|A0A5C0CH54|A0A5C0CH54_9CAUD DUF2724 domain-containing protein OS=Salmonella phage SI22 OX=2592209 PE=4 SV=1
MM1 pKa = 6.76 TTLDD5 pKa = 4.14 PRR7 pKa = 11.84 QLWRR11 pKa = 11.84 RR12 pKa = 11.84 TARR15 pKa = 11.84 TGKK18 pKa = 10.2 SDD20 pKa = 3.01 ACKK23 pKa = 10.13 TMHH26 pKa = 7.22 LLHH29 pKa = 7.04 ARR31 pKa = 11.84 LKK33 pKa = 9.09 NGKK36 pKa = 8.57 FAEE39 pKa = 4.39 EE40 pKa = 3.84 WHH42 pKa = 6.75 KK43 pKa = 10.64 KK44 pKa = 7.85 TGIHH48 pKa = 6.53 GAGLNQVYY56 pKa = 10.25
Molecular weight: 6.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.245
IPC2_protein 9.575
IPC_protein 9.78
Toseland 10.701
ProMoST 10.233
Dawson 10.774
Bjellqvist 10.379
Wikipedia 10.891
Rodwell 11.272
Grimsley 10.789
Solomon 10.833
Lehninger 10.818
Nozaki 10.672
DTASelect 10.365
Thurlkill 10.672
EMBOSS 11.082
Sillero 10.687
Patrickios 11.052
IPC_peptide 10.847
IPC2_peptide 9.063
IPC2.peptide.svr19 8.619
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
45
0
45
9566
39
814
212.6
23.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.391 ± 0.513
0.972 ± 0.137
6.251 ± 0.238
5.969 ± 0.337
3.084 ± 0.259
6.941 ± 0.46
1.955 ± 0.225
5.415 ± 0.195
5.614 ± 0.307
9.168 ± 0.279
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.519 ± 0.158
4.077 ± 0.222
4.087 ± 0.241
3.805 ± 0.316
6.293 ± 0.451
6.23 ± 0.255
6.324 ± 0.326
6.586 ± 0.33
1.662 ± 0.165
2.655 ± 0.2
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here