Bifidobacterium phage BitterVaud1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XZL1|A0A6G6XZL1_9CAUD Uncharacterized protein OS=Bifidobacterium phage BitterVaud1 OX=2713247 GN=BIHU0010003c01_00015 PE=4 SV=1
MM1 pKa = 7.55EE2 pKa = 4.75SAIGTTPSIAADD14 pKa = 3.59DD15 pKa = 4.41FMLTTTDD22 pKa = 3.36NPFNPWIDD30 pKa = 3.44FDD32 pKa = 3.31TWYY35 pKa = 10.94AEE37 pKa = 4.56DD38 pKa = 3.88IRR40 pKa = 11.84LGHH43 pKa = 6.02DD44 pKa = 2.89TCGYY48 pKa = 9.66LDD50 pKa = 3.45RR51 pKa = 11.84VMTGSSALTQGEE63 pKa = 4.51NNVSYY68 pKa = 11.51NDD70 pKa = 3.41ACNDD74 pKa = 4.1IIRR77 pKa = 11.84LDD79 pKa = 3.57PTGLYY84 pKa = 9.8TKK86 pKa = 10.12IKK88 pKa = 10.41KK89 pKa = 9.37PGKK92 pKa = 9.84

Molecular weight: 10.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6Y0K1|A0A6G6Y0K1_9CAUD Uncharacterized protein OS=Bifidobacterium phage BitterVaud1 OX=2713247 GN=BIHU0010003c01_00024 PE=4 SV=1
MM1 pKa = 7.38TRR3 pKa = 11.84TSYY6 pKa = 9.73WADD9 pKa = 3.63PLPEE13 pKa = 5.51DD14 pKa = 3.81SEE16 pKa = 4.47EE17 pKa = 4.26SLEE20 pKa = 4.16HH21 pKa = 6.56FGVKK25 pKa = 9.15GMKK28 pKa = 8.84WGVRR32 pKa = 11.84KK33 pKa = 9.97KK34 pKa = 9.99RR35 pKa = 11.84TGDD38 pKa = 3.15TLSRR42 pKa = 11.84VGKK45 pKa = 9.57AAKK48 pKa = 8.86STGRR52 pKa = 11.84AAAQVPKK59 pKa = 10.89VVGHH63 pKa = 6.85DD64 pKa = 3.52IYY66 pKa = 11.22RR67 pKa = 11.84KK68 pKa = 10.2ASDD71 pKa = 4.18RR72 pKa = 11.84GQQMYY77 pKa = 10.87EE78 pKa = 3.79KK79 pKa = 10.9GDD81 pKa = 3.63VQKK84 pKa = 10.74HH85 pKa = 3.97HH86 pKa = 6.74TIVRR90 pKa = 11.84AVGRR94 pKa = 11.84SVLYY98 pKa = 10.69GLGSRR103 pKa = 11.84AVGTALVFSGRR114 pKa = 11.84YY115 pKa = 3.97TTTAGKK121 pKa = 9.93VIKK124 pKa = 10.3AADD127 pKa = 3.52IGHH130 pKa = 6.72SILNSSLAANDD141 pKa = 3.67TVRR144 pKa = 11.84NMSLRR149 pKa = 11.84AYY151 pKa = 9.76EE152 pKa = 4.12HH153 pKa = 7.49DD154 pKa = 3.51LRR156 pKa = 11.84KK157 pKa = 10.17ANKK160 pKa = 7.06TTSKK164 pKa = 10.31KK165 pKa = 9.72QSS167 pKa = 3.08

Molecular weight: 18.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

16062

60

2019

239.7

26.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.658 ± 0.568

0.928 ± 0.206

7.708 ± 0.395

5.803 ± 0.426

3.505 ± 0.178

7.135 ± 0.678

2.216 ± 0.218

6.431 ± 0.218

6.039 ± 0.394

7.683 ± 0.413

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.764 ± 0.138

5.037 ± 0.193

3.879 ± 0.295

3.698 ± 0.183

5.441 ± 0.469

7.384 ± 0.432

5.404 ± 0.271

6.325 ± 0.252

1.37 ± 0.112

3.592 ± 0.327
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski