Lactobacillus antri DSM 16041
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2224 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C8P9V2|C8P9V2_9LACO ATP-grasp domain-containing protein OS=Lactobacillus antri DSM 16041 OX=525309 GN=HMPREF0494_2096 PE=4 SV=1
MM1 pKa = 8.56 DD2 pKa = 4.83 YY3 pKa = 7.59 MTLNNGEE10 pKa = 4.68 KK11 pKa = 10.3 VPQLGFDD18 pKa = 4.03 VYY20 pKa = 10.7 QIPNEE25 pKa = 4.16 EE26 pKa = 3.96 IKK28 pKa = 10.74 EE29 pKa = 4.06 AVYY32 pKa = 10.31 QAVTAGYY39 pKa = 10.17 RR40 pKa = 11.84 LIDD43 pKa = 3.51 TAVSYY48 pKa = 11.36 GNEE51 pKa = 4.07 AEE53 pKa = 4.33 VGAGVARR60 pKa = 11.84 AIADD64 pKa = 3.49 GLVSRR69 pKa = 11.84 EE70 pKa = 3.92 DD71 pKa = 4.29 LFITTCC77 pKa = 3.64
Molecular weight: 8.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.885
IPC2_protein 4.19
IPC_protein 4.037
Toseland 3.872
ProMoST 4.177
Dawson 3.999
Bjellqvist 4.164
Wikipedia 3.91
Rodwell 3.872
Grimsley 3.783
Solomon 3.986
Lehninger 3.935
Nozaki 4.126
DTASelect 4.266
Thurlkill 3.91
EMBOSS 3.923
Sillero 4.151
Patrickios 1.926
IPC_peptide 3.986
IPC2_peptide 4.139
IPC2.peptide.svr19 4.042
Protein with the highest isoelectric point:
>tr|C8P542|C8P542_9LACO UDP-N-acetylmuramyl-tripeptide synthetase OS=Lactobacillus antri DSM 16041 OX=525309 GN=murE PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPKK8 pKa = 8.02 KK9 pKa = 7.4 RR10 pKa = 11.84 HH11 pKa = 5.36 RR12 pKa = 11.84 ARR14 pKa = 11.84 VHH16 pKa = 5.84 GFRR19 pKa = 11.84 KK20 pKa = 10.03 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 KK29 pKa = 8.01 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.39 GRR39 pKa = 11.84 KK40 pKa = 8.52 VLSAA44 pKa = 4.05
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.522
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.735
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.457
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.169
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2224
0
2224
663820
39
2266
298.5
33.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.074 ± 0.077
0.641 ± 0.016
5.767 ± 0.056
4.985 ± 0.062
4.027 ± 0.04
6.987 ± 0.05
2.371 ± 0.026
6.31 ± 0.053
5.588 ± 0.052
9.767 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.551 ± 0.025
4.637 ± 0.049
3.943 ± 0.034
5.154 ± 0.051
4.764 ± 0.054
5.406 ± 0.048
5.985 ± 0.061
7.327 ± 0.045
1.137 ± 0.022
3.582 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here