Amino acid dipepetide frequency for Enterococcus casseliflavus EC20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.136AlaAla: 8.136 ± 0.138
0.614AlaCys: 0.614 ± 0.027
4.384AlaAsp: 4.384 ± 0.076
5.613AlaGlu: 5.613 ± 0.109
3.893AlaPhe: 3.893 ± 0.058
5.494AlaGly: 5.494 ± 0.091
1.333AlaHis: 1.333 ± 0.038
6.521AlaIle: 6.521 ± 0.098
4.865AlaLys: 4.865 ± 0.071
7.958AlaLeu: 7.958 ± 0.099
2.136AlaMet: 2.136 ± 0.051
3.175AlaAsn: 3.175 ± 0.064
2.504AlaPro: 2.504 ± 0.075
3.693AlaGln: 3.693 ± 0.071
2.704AlaArg: 2.704 ± 0.055
4.767AlaSer: 4.767 ± 0.073
4.49AlaThr: 4.49 ± 0.084
5.745AlaVal: 5.745 ± 0.086
0.683AlaTrp: 0.683 ± 0.026
2.767AlaTyr: 2.767 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
0.422CysAla: 0.422 ± 0.022
0.074CysCys: 0.074 ± 0.008
0.306CysAsp: 0.306 ± 0.02
0.325CysGlu: 0.325 ± 0.02
0.368CysPhe: 0.368 ± 0.02
0.618CysGly: 0.618 ± 0.03
0.169CysHis: 0.169 ± 0.013
0.363CysIle: 0.363 ± 0.019
0.214CysLys: 0.214 ± 0.016
0.73CysLeu: 0.73 ± 0.033
0.139CysMet: 0.139 ± 0.012
0.187CysAsn: 0.187 ± 0.013
0.267CysPro: 0.267 ± 0.018
0.346CysGln: 0.346 ± 0.021
0.272CysArg: 0.272 ± 0.02
0.384CysSer: 0.384 ± 0.022
0.33CysThr: 0.33 ± 0.018
0.363CysVal: 0.363 ± 0.019
0.063CysTrp: 0.063 ± 0.008
0.276CysTyr: 0.276 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
3.869AspAla: 3.869 ± 0.077
0.33AspCys: 0.33 ± 0.019
2.529AspAsp: 2.529 ± 0.055
4.131AspGlu: 4.131 ± 0.076
2.794AspPhe: 2.794 ± 0.057
3.539AspGly: 3.539 ± 0.068
1.127AspHis: 1.127 ± 0.036
3.236AspIle: 3.236 ± 0.055
2.556AspLys: 2.556 ± 0.072
5.142AspLeu: 5.142 ± 0.07
1.112AspMet: 1.112 ± 0.032
1.715AspAsn: 1.715 ± 0.05
2.352AspPro: 2.352 ± 0.05
2.749AspGln: 2.749 ± 0.058
2.21AspArg: 2.21 ± 0.052
2.73AspSer: 2.73 ± 0.061
2.937AspThr: 2.937 ± 0.081
3.42AspVal: 3.42 ± 0.063
0.677AspTrp: 0.677 ± 0.028
2.371AspTyr: 2.371 ± 0.054
0.0AspXaa: 0.0 ± 0.0
Glu
6.536GluAla: 6.536 ± 0.095
0.309GluCys: 0.309 ± 0.019
3.472GluAsp: 3.472 ± 0.07
6.746GluGlu: 6.746 ± 0.102
2.209GluPhe: 2.209 ± 0.056
4.056GluGly: 4.056 ± 0.085
1.122GluHis: 1.122 ± 0.039
5.168GluIle: 5.168 ± 0.084
5.906GluLys: 5.906 ± 0.094
6.892GluLeu: 6.892 ± 0.1
2.28GluMet: 2.28 ± 0.045
3.634GluAsn: 3.634 ± 0.061
2.267GluPro: 2.267 ± 0.061
3.633GluGln: 3.633 ± 0.067
3.077GluArg: 3.077 ± 0.056
3.34GluSer: 3.34 ± 0.069
4.934GluThr: 4.934 ± 0.066
4.835GluVal: 4.835 ± 0.078
0.737GluTrp: 0.737 ± 0.03
2.074GluTyr: 2.074 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
3.665PheAla: 3.665 ± 0.068
0.36PheCys: 0.36 ± 0.021
2.847PheAsp: 2.847 ± 0.061
2.949PheGlu: 2.949 ± 0.059
2.736PhePhe: 2.736 ± 0.056
3.32PheGly: 3.32 ± 0.062
0.905PheHis: 0.905 ± 0.033
2.987PheIle: 2.987 ± 0.07
1.89PheLys: 1.89 ± 0.047
5.241PheLeu: 5.241 ± 0.096
1.082PheMet: 1.082 ± 0.035
1.675PheAsn: 1.675 ± 0.043
1.732PhePro: 1.732 ± 0.048
1.979PheGln: 1.979 ± 0.05
1.628PheArg: 1.628 ± 0.051
3.517PheSer: 3.517 ± 0.062
2.669PheThr: 2.669 ± 0.055
3.418PheVal: 3.418 ± 0.054
0.542PheTrp: 0.542 ± 0.02
1.995PheTyr: 1.995 ± 0.05
0.0PheXaa: 0.0 ± 0.0
Gly
4.821GlyAla: 4.821 ± 0.1
0.513GlyCys: 0.513 ± 0.023
3.09GlyAsp: 3.09 ± 0.067
4.17GlyGlu: 4.17 ± 0.07
3.366GlyPhe: 3.366 ± 0.068
4.575GlyGly: 4.575 ± 0.098
1.174GlyHis: 1.174 ± 0.039
5.738GlyIle: 5.738 ± 0.099
4.208GlyLys: 4.208 ± 0.068
6.651GlyLeu: 6.651 ± 0.086
1.984GlyMet: 1.984 ± 0.048
2.827GlyAsn: 2.827 ± 0.072
1.594GlyPro: 1.594 ± 0.04
2.517GlyGln: 2.517 ± 0.058
2.433GlyArg: 2.433 ± 0.056
3.9GlySer: 3.9 ± 0.075
4.243GlyThr: 4.243 ± 0.1
4.82GlyVal: 4.82 ± 0.069
0.766GlyTrp: 0.766 ± 0.031
2.654GlyTyr: 2.654 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
1.2HisAla: 1.2 ± 0.041
0.158HisCys: 0.158 ± 0.013
0.905HisAsp: 0.905 ± 0.033
1.285HisGlu: 1.285 ± 0.043
1.109HisPhe: 1.109 ± 0.035
1.251HisGly: 1.251 ± 0.043
0.484HisHis: 0.484 ± 0.023
1.037HisIle: 1.037 ± 0.036
0.688HisLys: 0.688 ± 0.027
2.087HisLeu: 2.087 ± 0.052
0.394HisMet: 0.394 ± 0.021
0.562HisAsn: 0.562 ± 0.024
0.994HisPro: 0.994 ± 0.034
1.061HisGln: 1.061 ± 0.037
0.781HisArg: 0.781 ± 0.025
0.981HisSer: 0.981 ± 0.032
0.972HisThr: 0.972 ± 0.033
1.132HisVal: 1.132 ± 0.033
0.24HisTrp: 0.24 ± 0.017
0.901HisTyr: 0.901 ± 0.034
0.0HisXaa: 0.0 ± 0.0
Ile
6.432IleAla: 6.432 ± 0.096
0.545IleCys: 0.545 ± 0.023
4.666IleAsp: 4.666 ± 0.065
5.054IleGlu: 5.054 ± 0.085
3.112IlePhe: 3.112 ± 0.067
5.434IleGly: 5.434 ± 0.096
1.45IleHis: 1.45 ± 0.035
4.766IleIle: 4.766 ± 0.086
3.817IleLys: 3.817 ± 0.07
6.606IleLeu: 6.606 ± 0.086
1.526IleMet: 1.526 ± 0.038
3.118IleAsn: 3.118 ± 0.063
3.082IlePro: 3.082 ± 0.059
3.088IleGln: 3.088 ± 0.064
2.93IleArg: 2.93 ± 0.053
4.443IleSer: 4.443 ± 0.068
4.196IleThr: 4.196 ± 0.075
5.149IleVal: 5.149 ± 0.091
0.546IleTrp: 0.546 ± 0.023
2.436IleTyr: 2.436 ± 0.052
0.0IleXaa: 0.0 ± 0.0
Lys
4.609LysAla: 4.609 ± 0.075
0.209LysCys: 0.209 ± 0.015
3.123LysAsp: 3.123 ± 0.068
6.26LysGlu: 6.26 ± 0.073
1.635LysPhe: 1.635 ± 0.044
3.377LysGly: 3.377 ± 0.062
0.995LysHis: 0.995 ± 0.036
4.33LysIle: 4.33 ± 0.069
5.209LysLys: 5.209 ± 0.089
4.545LysLeu: 4.545 ± 0.077
1.899LysMet: 1.899 ± 0.045
3.015LysAsn: 3.015 ± 0.062
1.754LysPro: 1.754 ± 0.042
3.11LysGln: 3.11 ± 0.07
2.835LysArg: 2.835 ± 0.054
2.525LysSer: 2.525 ± 0.05
3.498LysThr: 3.498 ± 0.06
3.705LysVal: 3.705 ± 0.061
0.488LysTrp: 0.488 ± 0.022
1.745LysTyr: 1.745 ± 0.044
0.0LysXaa: 0.0 ± 0.0
Leu
9.345LeuAla: 9.345 ± 0.123
0.65LeuCys: 0.65 ± 0.026
4.778LeuAsp: 4.778 ± 0.069
5.942LeuGlu: 5.942 ± 0.091
5.293LeuPhe: 5.293 ± 0.083
6.345LeuGly: 6.345 ± 0.092
1.657LeuHis: 1.657 ± 0.047
7.006LeuIle: 7.006 ± 0.105
5.404LeuLys: 5.404 ± 0.084
11.262LeuLeu: 11.262 ± 0.155
2.483LeuMet: 2.483 ± 0.054
3.867LeuAsn: 3.867 ± 0.067
4.527LeuPro: 4.527 ± 0.07
4.276LeuGln: 4.276 ± 0.085
3.753LeuArg: 3.753 ± 0.07
6.827LeuSer: 6.827 ± 0.092
6.63LeuThr: 6.63 ± 0.085
6.619LeuVal: 6.619 ± 0.086
0.888LeuTrp: 0.888 ± 0.03
3.115LeuTyr: 3.115 ± 0.054
0.0LeuXaa: 0.0 ± 0.0
Met
2.151MetAla: 2.151 ± 0.044
0.113MetCys: 0.113 ± 0.011
1.33MetAsp: 1.33 ± 0.037
1.614MetGlu: 1.614 ± 0.042
0.884MetPhe: 0.884 ± 0.032
1.622MetGly: 1.622 ± 0.045
0.368MetHis: 0.368 ± 0.019
2.265MetIle: 2.265 ± 0.05
2.113MetLys: 2.113 ± 0.047
2.257MetLeu: 2.257 ± 0.053
0.839MetMet: 0.839 ± 0.03
1.397MetAsn: 1.397 ± 0.034
0.968MetPro: 0.968 ± 0.03
0.978MetGln: 0.978 ± 0.036
1.002MetArg: 1.002 ± 0.031
1.418MetSer: 1.418 ± 0.035
1.806MetThr: 1.806 ± 0.042
1.794MetVal: 1.794 ± 0.043
0.177MetTrp: 0.177 ± 0.014
0.643MetTyr: 0.643 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
2.825AsnAla: 2.825 ± 0.055
0.265AsnCys: 0.265 ± 0.018
2.221AsnAsp: 2.221 ± 0.049
3.257AsnGlu: 3.257 ± 0.061
1.726AsnPhe: 1.726 ± 0.05
3.126AsnGly: 3.126 ± 0.067
1.028AsnHis: 1.028 ± 0.033
2.641AsnIle: 2.641 ± 0.053
2.164AsnLys: 2.164 ± 0.048
3.637AsnLeu: 3.637 ± 0.065
0.973AsnMet: 0.973 ± 0.031
1.732AsnAsn: 1.732 ± 0.057
1.9AsnPro: 1.9 ± 0.045
2.7AsnGln: 2.7 ± 0.054
1.866AsnArg: 1.866 ± 0.049
2.111AsnSer: 2.111 ± 0.057
2.22AsnThr: 2.22 ± 0.051
2.722AsnVal: 2.722 ± 0.063
0.5AsnTrp: 0.5 ± 0.027
1.72AsnTyr: 1.72 ± 0.042
0.0AsnXaa: 0.0 ± 0.0
Pro
2.883ProAla: 2.883 ± 0.073
0.167ProCys: 0.167 ± 0.014
2.053ProAsp: 2.053 ± 0.049
3.454ProGlu: 3.454 ± 0.066
2.034ProPhe: 2.034 ± 0.046
1.959ProGly: 1.959 ± 0.048
0.689ProHis: 0.689 ± 0.026
2.859ProIle: 2.859 ± 0.057
2.073ProLys: 2.073 ± 0.043
3.794ProLeu: 3.794 ± 0.068
0.912ProMet: 0.912 ± 0.03
1.644ProAsn: 1.644 ± 0.048
0.792ProPro: 0.792 ± 0.031
1.69ProGln: 1.69 ± 0.042
1.085ProArg: 1.085 ± 0.037
2.107ProSer: 2.107 ± 0.057
2.393ProThr: 2.393 ± 0.058
2.692ProVal: 2.692 ± 0.061
0.353ProTrp: 0.353 ± 0.02
1.402ProTyr: 1.402 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
4.437GlnAla: 4.437 ± 0.088
0.192GlnCys: 0.192 ± 0.015
1.81GlnAsp: 1.81 ± 0.047
4.053GlnGlu: 4.053 ± 0.061
1.7GlnPhe: 1.7 ± 0.043
2.682GlnGly: 2.682 ± 0.053
0.758GlnHis: 0.758 ± 0.031
2.938GlnIle: 2.938 ± 0.057
3.173GlnLys: 3.173 ± 0.068
5.218GlnLeu: 5.218 ± 0.093
1.309GlnMet: 1.309 ± 0.044
1.739GlnAsn: 1.739 ± 0.047
1.651GlnPro: 1.651 ± 0.04
3.056GlnGln: 3.056 ± 0.085
2.166GlnArg: 2.166 ± 0.054
2.394GlnSer: 2.394 ± 0.054
3.004GlnThr: 3.004 ± 0.057
3.195GlnVal: 3.195 ± 0.062
0.551GlnTrp: 0.551 ± 0.029
1.285GlnTyr: 1.285 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
2.347ArgAla: 2.347 ± 0.05
0.209ArgCys: 0.209 ± 0.016
1.713ArgAsp: 1.713 ± 0.046
2.84ArgGlu: 2.84 ± 0.052
2.241ArgPhe: 2.241 ± 0.048
2.184ArgGly: 2.184 ± 0.046
0.697ArgHis: 0.697 ± 0.025
2.909ArgIle: 2.909 ± 0.062
2.762ArgLys: 2.762 ± 0.055
4.294ArgLeu: 4.294 ± 0.077
1.149ArgMet: 1.149 ± 0.032
1.702ArgAsn: 1.702 ± 0.044
1.402ArgPro: 1.402 ± 0.043
2.053ArgGln: 2.053 ± 0.048
1.804ArgArg: 1.804 ± 0.051
2.294ArgSer: 2.294 ± 0.054
2.149ArgThr: 2.149 ± 0.044
2.45ArgVal: 2.45 ± 0.055
0.391ArgTrp: 0.391 ± 0.021
1.741ArgTyr: 1.741 ± 0.049
0.0ArgXaa: 0.0 ± 0.0
Ser
4.244SerAla: 4.244 ± 0.086
0.324SerCys: 0.324 ± 0.019
2.9SerAsp: 2.9 ± 0.059
4.266SerGlu: 4.266 ± 0.064
3.293SerPhe: 3.293 ± 0.062
4.415SerGly: 4.415 ± 0.079
1.047SerHis: 1.047 ± 0.039
4.296SerIle: 4.296 ± 0.071
3.021SerLys: 3.021 ± 0.055
6.053SerLeu: 6.053 ± 0.092
1.444SerMet: 1.444 ± 0.04
2.396SerAsn: 2.396 ± 0.061
1.888SerPro: 1.888 ± 0.043
2.517SerGln: 2.517 ± 0.05
2.187SerArg: 2.187 ± 0.049
3.901SerSer: 3.901 ± 0.108
3.342SerThr: 3.342 ± 0.073
3.838SerVal: 3.838 ± 0.063
0.633SerTrp: 0.633 ± 0.024
2.301SerTyr: 2.301 ± 0.052
0.0SerXaa: 0.0 ± 0.0
Thr
4.963ThrAla: 4.963 ± 0.136
0.308ThrCys: 0.308 ± 0.017
3.244ThrAsp: 3.244 ± 0.078
3.936ThrGlu: 3.936 ± 0.071
2.946ThrPhe: 2.946 ± 0.068
4.236ThrGly: 4.236 ± 0.077
1.058ThrHis: 1.058 ± 0.029
5.525ThrIle: 5.525 ± 0.075
3.304ThrLys: 3.304 ± 0.069
5.773ThrLeu: 5.773 ± 0.086
1.427ThrMet: 1.427 ± 0.036
2.601ThrAsn: 2.601 ± 0.052
2.647ThrPro: 2.647 ± 0.063
2.178ThrGln: 2.178 ± 0.048
1.936ThrArg: 1.936 ± 0.047
3.486ThrSer: 3.486 ± 0.074
3.891ThrThr: 3.891 ± 0.088
4.614ThrVal: 4.614 ± 0.144
0.555ThrTrp: 0.555 ± 0.027
2.263ThrTyr: 2.263 ± 0.057
0.0ThrXaa: 0.0 ± 0.0
Val
5.633ValAla: 5.633 ± 0.093
0.525ValCys: 0.525 ± 0.022
3.674ValAsp: 3.674 ± 0.065
4.345ValGlu: 4.345 ± 0.067
3.341ValPhe: 3.341 ± 0.061
4.628ValGly: 4.628 ± 0.082
1.061ValHis: 1.061 ± 0.034
5.312ValIle: 5.312 ± 0.08
3.647ValLys: 3.647 ± 0.064
6.998ValLeu: 6.998 ± 0.086
1.696ValMet: 1.696 ± 0.044
2.659ValAsn: 2.659 ± 0.058
2.859ValPro: 2.859 ± 0.059
2.405ValGln: 2.405 ± 0.053
2.437ValArg: 2.437 ± 0.054
4.519ValSer: 4.519 ± 0.08
4.668ValThr: 4.668 ± 0.122
5.087ValVal: 5.087 ± 0.105
0.584ValTrp: 0.584 ± 0.026
2.333ValTyr: 2.333 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
0.601TrpAla: 0.601 ± 0.028
0.062TrpCys: 0.062 ± 0.008
0.471TrpAsp: 0.471 ± 0.023
0.622TrpGlu: 0.622 ± 0.026
0.518TrpPhe: 0.518 ± 0.024
0.688TrpGly: 0.688 ± 0.03
0.212TrpHis: 0.212 ± 0.016
0.604TrpIle: 0.604 ± 0.028
0.43TrpLys: 0.43 ± 0.022
1.313TrpLeu: 1.313 ± 0.039
0.249TrpMet: 0.249 ± 0.013
0.43TrpAsn: 0.43 ± 0.02
0.273TrpPro: 0.273 ± 0.017
0.807TrpGln: 0.807 ± 0.038
0.43TrpArg: 0.43 ± 0.018
0.606TrpSer: 0.606 ± 0.029
0.556TrpThr: 0.556 ± 0.025
0.593TrpVal: 0.593 ± 0.027
0.127TrpTrp: 0.127 ± 0.01
0.345TrpTyr: 0.345 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.357TyrAla: 2.357 ± 0.05
0.289TyrCys: 0.289 ± 0.018
2.009TyrAsp: 2.009 ± 0.049
2.365TyrGlu: 2.365 ± 0.053
1.939TyrPhe: 1.939 ± 0.045
2.496TyrGly: 2.496 ± 0.051
0.887TyrHis: 0.887 ± 0.032
1.897TyrIle: 1.897 ± 0.052
1.423TyrLys: 1.423 ± 0.039
4.217TyrLeu: 4.217 ± 0.071
0.706TyrMet: 0.706 ± 0.032
1.259TyrAsn: 1.259 ± 0.035
1.583TyrPro: 1.583 ± 0.04
2.473TyrGln: 2.473 ± 0.05
1.797TyrArg: 1.797 ± 0.043
2.056TyrSer: 2.056 ± 0.053
1.96TyrThr: 1.96 ± 0.052
2.222TyrVal: 2.222 ± 0.051
0.431TyrTrp: 0.431 ± 0.022
1.558TyrTyr: 1.558 ± 0.043
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3112 proteins (993929 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski