Streptomonospora alba
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4307 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C2FEN2|A0A0C2FEN2_9ACTN Uncharacterized protein OS=Streptomonospora alba OX=183763 GN=LP52_18155 PE=4 SV=1
MM1 pKa = 7.42 SPSSSRR7 pKa = 11.84 SRR9 pKa = 11.84 FAAGAVTAASAIALSMLAGAPGAHH33 pKa = 6.75 AAEE36 pKa = 4.69 LVTNGGFDD44 pKa = 3.45 NGTTGWWNTEE54 pKa = 3.64 NAPAEE59 pKa = 4.34 VADD62 pKa = 4.29 GRR64 pKa = 11.84 LCADD68 pKa = 3.72 VEE70 pKa = 4.5 GGTANAWDD78 pKa = 5.1 AIVGQDD84 pKa = 4.84 GIALTSGEE92 pKa = 4.4 SYY94 pKa = 10.96 EE95 pKa = 4.3 FTFTASASEE104 pKa = 4.31 GTTVRR109 pKa = 11.84 ALIQEE114 pKa = 3.99 PTEE117 pKa = 4.43 PYY119 pKa = 9.73 TAFLTANPALTSEE132 pKa = 4.5 TQTFSHH138 pKa = 7.02 TFTADD143 pKa = 2.85 ATLDD147 pKa = 3.63 DD148 pKa = 4.44 AQLVFQLGGEE158 pKa = 4.14 AEE160 pKa = 4.08 AWTFCLDD167 pKa = 4.62 DD168 pKa = 4.65 VSLQDD173 pKa = 3.54 GAEE176 pKa = 4.08 PPVYY180 pKa = 10.25 EE181 pKa = 5.39 PDD183 pKa = 2.95 TGPPVKK189 pKa = 10.39 VNQVGYY195 pKa = 10.69 LPDD198 pKa = 3.93 GPKK201 pKa = 10.39 NATLVTDD208 pKa = 4.1 ATSAQPWTLRR218 pKa = 11.84 DD219 pKa = 3.23 ADD221 pKa = 3.87 GAAVAEE227 pKa = 4.93 GQTSPQGTEE236 pKa = 3.55 QSSGQNVHH244 pKa = 6.84 SIDD247 pKa = 3.67 FSDD250 pKa = 3.88 YY251 pKa = 8.24 TEE253 pKa = 4.61 KK254 pKa = 11.12 GSGYY258 pKa = 8.19 TLAVGDD264 pKa = 4.52 DD265 pKa = 3.59 VSHH268 pKa = 7.21 PFDD271 pKa = 3.73 ISGSAYY277 pKa = 9.65 KK278 pKa = 10.41 DD279 pKa = 3.35 LRR281 pKa = 11.84 TDD283 pKa = 3.43 SLSLYY288 pKa = 8.96 YY289 pKa = 9.36 PQRR292 pKa = 11.84 SGIEE296 pKa = 3.81 IDD298 pKa = 3.92 DD299 pKa = 4.04 SLMPGYY305 pKa = 10.28 GRR307 pKa = 11.84 AAGHH311 pKa = 6.28 VGVEE315 pKa = 4.23 PNQGDD320 pKa = 3.96 LSVPCAPDD328 pKa = 3.18 SGCDD332 pKa = 3.53 YY333 pKa = 10.94 EE334 pKa = 6.2 LDD336 pKa = 4.33 VSGGWYY342 pKa = 10.02 DD343 pKa = 3.88 AGDD346 pKa = 3.49 HH347 pKa = 6.07 GKK349 pKa = 10.25 YY350 pKa = 9.61 VVNGGISASQVMSVWEE366 pKa = 4.04 RR367 pKa = 11.84 TTYY370 pKa = 11.23 ADD372 pKa = 3.19 SASPDD377 pKa = 3.35 RR378 pKa = 11.84 LGDD381 pKa = 3.3 GTLPVPEE388 pKa = 4.72 QGNDD392 pKa = 3.28 VPDD395 pKa = 3.92 VLDD398 pKa = 3.64 EE399 pKa = 4.8 ARR401 pKa = 11.84 WEE403 pKa = 4.06 MDD405 pKa = 3.19 FLMKK409 pKa = 9.91 MQVPEE414 pKa = 4.27 GEE416 pKa = 4.34 EE417 pKa = 3.79 LAGMAHH423 pKa = 6.57 HH424 pKa = 6.95 KK425 pKa = 9.3 MHH427 pKa = 7.0 DD428 pKa = 4.03 EE429 pKa = 4.01 EE430 pKa = 4.82 WTGLPLMPADD440 pKa = 4.69 DD441 pKa = 4.28 PQPRR445 pKa = 11.84 YY446 pKa = 9.43 LQPPSTAATLNLAATGAQCARR467 pKa = 11.84 VFEE470 pKa = 5.01 SYY472 pKa = 10.87 DD473 pKa = 3.25 SEE475 pKa = 4.31 FADD478 pKa = 3.59 RR479 pKa = 11.84 CLEE482 pKa = 4.09 TAEE485 pKa = 4.9 KK486 pKa = 10.02 AWQAAQEE493 pKa = 4.29 NPDD496 pKa = 3.68 VLADD500 pKa = 3.78 PEE502 pKa = 4.31 NGVGGGAYY510 pKa = 10.23 SDD512 pKa = 5.21 DD513 pKa = 3.83 DD514 pKa = 4.72 VSDD517 pKa = 3.53 EE518 pKa = 4.94 FYY520 pKa = 10.34 WAAAEE525 pKa = 4.28 LFITTGEE532 pKa = 3.91 QSYY535 pKa = 11.18 EE536 pKa = 4.06 DD537 pKa = 3.6 AVLDD541 pKa = 4.34 HH542 pKa = 6.89 EE543 pKa = 4.93 LHH545 pKa = 6.05 TEE547 pKa = 4.07 DD548 pKa = 4.55 VFTAGGLAWGAVAPLGRR565 pKa = 11.84 MNLALVPNKK574 pKa = 10.33 LDD576 pKa = 3.68 DD577 pKa = 3.94 RR578 pKa = 11.84 DD579 pKa = 3.78 RR580 pKa = 11.84 VVDD583 pKa = 4.07 SVVAGADD590 pKa = 3.51 EE591 pKa = 4.16 YY592 pKa = 11.71 LDD594 pKa = 3.53 TLRR597 pKa = 11.84 NHH599 pKa = 7.2 AFGLPYY605 pKa = 10.52 APPDD609 pKa = 3.52 NVFVWGSNSQVLNNMVVMTAAYY631 pKa = 10.33 DD632 pKa = 3.72 LTGDD636 pKa = 3.57 GAYY639 pKa = 10.07 RR640 pKa = 11.84 DD641 pKa = 4.1 GVLEE645 pKa = 4.21 GMDD648 pKa = 3.88 YY649 pKa = 10.92 ILGRR653 pKa = 11.84 NALGQSYY660 pKa = 7.56 VTGYY664 pKa = 11.12 GEE666 pKa = 3.97 QFSKK670 pKa = 10.56 NQHH673 pKa = 4.93 SRR675 pKa = 11.84 WYY677 pKa = 10.58 ANQLDD682 pKa = 4.36 ADD684 pKa = 4.48 LPNPPEE690 pKa = 4.32 GTVSGGPNSQTSTWDD705 pKa = 3.09 PVAQDD710 pKa = 4.0 KK711 pKa = 11.73 LEE713 pKa = 4.31 GCAPQFCYY721 pKa = 10.09 IDD723 pKa = 5.73 DD724 pKa = 4.03 IEE726 pKa = 4.27 SWATNEE732 pKa = 5.0 LTVNWNAPLAIVSSFIADD750 pKa = 3.36 QTGEE754 pKa = 4.23 GDD756 pKa = 3.84 PPAEE760 pKa = 5.1 DD761 pKa = 3.48 EE762 pKa = 4.53 TAPTAPGKK770 pKa = 9.16 PQVSGVTSSQAEE782 pKa = 4.45 LTWEE786 pKa = 4.06 ASTDD790 pKa = 3.44 EE791 pKa = 4.73 GGSGVEE797 pKa = 4.61 GYY799 pKa = 10.24 DD800 pKa = 3.44 VYY802 pKa = 11.57 AGTGDD807 pKa = 3.75 GAQKK811 pKa = 10.49 VGSASGPSFTLTGLEE826 pKa = 4.28 SEE828 pKa = 4.67 TEE830 pKa = 4.24 YY831 pKa = 10.5 TVHH834 pKa = 6.25 VVARR838 pKa = 11.84 DD839 pKa = 3.52 GAGNRR844 pKa = 11.84 SEE846 pKa = 4.81 PGPNAAFTTEE856 pKa = 3.69 AGGTGGEE863 pKa = 4.24 AACEE867 pKa = 3.82 VDD869 pKa = 4.71 YY870 pKa = 10.42 STNDD874 pKa = 2.96 WNGGFTASVSVVNTGDD890 pKa = 3.29 TPIRR894 pKa = 11.84 DD895 pKa = 3.38 WEE897 pKa = 4.37 LGFEE901 pKa = 4.7 FGAGQEE907 pKa = 4.24 ITHH910 pKa = 6.43 GWNAEE915 pKa = 3.61 WSQDD919 pKa = 3.14 GSAVTASGLSWNSDD933 pKa = 3.7 LAPGSSVSAGFNGTAQDD950 pKa = 3.79 GNPAPEE956 pKa = 4.09 EE957 pKa = 4.01 FTVNGQTCAA966 pKa = 3.44
Molecular weight: 101.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.695
IPC2_protein 3.77
IPC_protein 3.808
Toseland 3.592
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.49
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.126
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.923
Patrickios 1.113
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.817
Protein with the highest isoelectric point:
>tr|A0A0C2FMM6|A0A0C2FMM6_9ACTN Hydrolase OS=Streptomonospora alba OX=183763 GN=LP52_00515 PE=3 SV=1
MM1 pKa = 7.49 RR2 pKa = 11.84 HH3 pKa = 5.9 SLLGGDD9 pKa = 3.6 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 PLGFGRR19 pKa = 11.84 LTRR22 pKa = 11.84 IGAVAGGAVARR33 pKa = 11.84 GNIAVGVAVRR43 pKa = 11.84 GTVRR47 pKa = 11.84 TVARR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 VHH55 pKa = 6.08 IPVRR59 pKa = 11.84 RR60 pKa = 11.84 GVRR63 pKa = 11.84 IPVRR67 pKa = 11.84 GAEE70 pKa = 4.04 PVAVVAARR78 pKa = 11.84 VSAVSVAAGTAAGVVRR94 pKa = 11.84 LL95 pKa = 4.12
Molecular weight: 9.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.439
IPC2_protein 10.95
IPC_protein 12.574
Toseland 12.72
ProMoST 13.232
Dawson 12.72
Bjellqvist 12.72
Wikipedia 13.203
Rodwell 12.223
Grimsley 12.764
Solomon 13.232
Lehninger 13.13
Nozaki 12.72
DTASelect 12.72
Thurlkill 12.72
EMBOSS 13.232
Sillero 12.72
Patrickios 11.959
IPC_peptide 13.232
IPC2_peptide 12.223
IPC2.peptide.svr19 9.159
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4307
0
4307
1345555
28
2499
312.4
33.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.665 ± 0.062
0.774 ± 0.011
6.261 ± 0.036
6.575 ± 0.036
2.78 ± 0.021
9.574 ± 0.04
2.198 ± 0.017
3.281 ± 0.024
1.599 ± 0.022
10.053 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.946 ± 0.015
1.727 ± 0.018
5.834 ± 0.033
2.695 ± 0.024
8.271 ± 0.038
5.317 ± 0.029
5.468 ± 0.024
8.492 ± 0.029
1.428 ± 0.015
2.063 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here