Atopobium deltae
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1442 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A133XSJ3|A0A133XSJ3_9ACTN Uncharacterized protein OS=Atopobium deltae OX=1393034 GN=HMPREF3192_01127 PE=4 SV=1
MM1 pKa = 6.91 TQLDD5 pKa = 3.93 SFIFKK10 pKa = 10.12 IDD12 pKa = 3.53 DD13 pKa = 3.52 RR14 pKa = 11.84 LEE16 pKa = 4.39 GVGATLSLDD25 pKa = 3.18 GRR27 pKa = 11.84 LAQAQFDD34 pKa = 4.27 LGDD37 pKa = 3.78 HH38 pKa = 6.24 EE39 pKa = 5.27 FVLDD43 pKa = 4.27 NGIAYY48 pKa = 10.41 NLVLTNTAEE57 pKa = 5.0 GILLTGIVKK66 pKa = 10.36 ADD68 pKa = 3.47 ATCPCDD74 pKa = 3.6 RR75 pKa = 11.84 CCEE78 pKa = 3.91 PAQLQLSCEE87 pKa = 3.75 VDD89 pKa = 3.09 EE90 pKa = 5.33 YY91 pKa = 11.95 YY92 pKa = 11.11 LFNKK96 pKa = 9.74 PEE98 pKa = 4.11 KK99 pKa = 10.18 QHH101 pKa = 6.45 LTDD104 pKa = 4.65 LDD106 pKa = 3.72 EE107 pKa = 5.26 EE108 pKa = 4.49 EE109 pKa = 4.99 DD110 pKa = 3.39 VDD112 pKa = 4.07 YY113 pKa = 11.7 SLVSAEE119 pKa = 4.04 NTIDD123 pKa = 3.76 LAPAIVSALVMEE135 pKa = 4.52 VPYY138 pKa = 10.45 IVLCDD143 pKa = 4.06 DD144 pKa = 4.67 DD145 pKa = 5.94 CLGLCSRR152 pKa = 11.84 CGANLNEE159 pKa = 4.63 GSCEE163 pKa = 4.09 CEE165 pKa = 3.41 RR166 pKa = 11.84 GANADD171 pKa = 3.6 GADD174 pKa = 4.13 AGNAAYY180 pKa = 10.04 DD181 pKa = 4.02 DD182 pKa = 4.19 NGDD185 pKa = 4.85 PIVQDD190 pKa = 3.87 EE191 pKa = 4.39 RR192 pKa = 11.84 SKK194 pKa = 10.81 PIPEE198 pKa = 4.75 GPFSALGKK206 pKa = 9.5 LHH208 pKa = 6.98 FDD210 pKa = 3.74 EE211 pKa = 5.29
Molecular weight: 22.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.753
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.592
ProMoST 3.948
Dawson 3.795
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.63
Grimsley 3.49
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.126
Thurlkill 3.63
EMBOSS 3.732
Sillero 3.923
Patrickios 0.604
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.836
Protein with the highest isoelectric point:
>tr|A0A133XVH1|A0A133XVH1_9ACTN Uncharacterized protein OS=Atopobium deltae OX=1393034 GN=HMPREF3192_00683 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.3 QPNKK9 pKa = 8.42 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 AKK14 pKa = 8.97 THH16 pKa = 5.3 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.18 GGRR28 pKa = 11.84 DD29 pKa = 3.3 VLSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.83 GRR39 pKa = 11.84 KK40 pKa = 8.88 RR41 pKa = 11.84 LTVV44 pKa = 3.11
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 10.906
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.193
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.93
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.081
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1442
0
1442
428962
34
1480
297.5
32.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.499 ± 0.074
1.492 ± 0.024
6.031 ± 0.06
5.794 ± 0.058
3.794 ± 0.039
6.748 ± 0.056
2.296 ± 0.03
5.912 ± 0.053
5.213 ± 0.061
9.363 ± 0.066
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.52 ± 0.029
3.457 ± 0.046
4.021 ± 0.038
4.181 ± 0.05
5.187 ± 0.055
6.342 ± 0.05
5.996 ± 0.05
7.22 ± 0.057
0.977 ± 0.019
2.956 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here