Tomato chlorosis virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Crinivirus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4KRZ1|Q4KRZ1_9CLOS p4 OS=Tomato chlorosis virus OX=67754 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.37LVCLVLRR8 pKa = 11.84TNCLVKK14 pKa = 10.6VLYY17 pKa = 10.53FSDD20 pKa = 3.85TPIVEE25 pKa = 4.33SGPLVIRR32 pKa = 11.84SSDD35 pKa = 3.29PTIIEE40 pKa = 4.62DD41 pKa = 3.82LLEE44 pKa = 3.95YY45 pKa = 10.77LPFVTEE51 pKa = 3.74SWNPLILPKK60 pKa = 10.24EE61 pKa = 4.4SKK63 pKa = 10.66DD64 pKa = 3.76FSQQ67 pKa = 4.59

Molecular weight: 7.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4KRZ4|Q4KRZ4_9CLOS RNA dependent RNA polymerase (Fragment) OS=Tomato chlorosis virus OX=67754 GN=ORF1b PE=4 SV=1
MM1 pKa = 7.09PTAGMLQPLLHH12 pKa = 6.15FVIVYY17 pKa = 9.83VMFSPIPPYY26 pKa = 11.08SFFVKK31 pKa = 10.63LKK33 pKa = 10.3

Molecular weight: 3.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

2

13

5165

33

1945

397.3

45.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.414 ± 0.286

2.033 ± 0.297

6.602 ± 0.227

5.015 ± 0.389

6.254 ± 0.338

5.344 ± 0.256

1.723 ± 0.277

6.486 ± 0.433

6.873 ± 0.553

9.332 ± 0.509

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.478 ± 0.351

5.557 ± 0.472

3.601 ± 0.288

2.285 ± 0.169

5.44 ± 0.191

8.267 ± 0.335

5.189 ± 0.338

7.861 ± 0.463

0.794 ± 0.128

4.395 ± 0.414
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski