Beihai weivirus-like virus 7
Average proteome isoelectric point is 7.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KL57|A0A1L3KL57_9VIRU Uncharacterized protein OS=Beihai weivirus-like virus 7 OX=1922754 PE=4 SV=1
MM1 pKa = 7.58 LPFSLDD7 pKa = 2.77 RR8 pKa = 11.84 SYY10 pKa = 11.73 RR11 pKa = 11.84 EE12 pKa = 4.02 LFLVEE17 pKa = 3.3 PWIRR21 pKa = 11.84 EE22 pKa = 3.9 VASNRR27 pKa = 11.84 EE28 pKa = 3.65 GDD30 pKa = 3.71 YY31 pKa = 11.05 KK32 pKa = 10.46 LTVFARR38 pKa = 11.84 HH39 pKa = 5.36 WFPWLWCSKK48 pKa = 9.55 KK49 pKa = 10.54 QNAEE53 pKa = 3.54 AFSIVFTGKK62 pKa = 9.0 EE63 pKa = 3.68 WSSLSFCIEE72 pKa = 3.63 HH73 pKa = 7.41 ALRR76 pKa = 11.84 TGLKK80 pKa = 9.17 EE81 pKa = 3.82 ANAYY85 pKa = 9.43 GAFVSKK91 pKa = 10.42 AQQLWPGGSQEE102 pKa = 3.97 EE103 pKa = 4.72 VAMKK107 pKa = 9.67 MLAPHH112 pKa = 6.37 MVLQYY117 pKa = 9.19 LTYY120 pKa = 9.86 KK121 pKa = 10.27 RR122 pKa = 11.84 VVVDD126 pKa = 3.51 HH127 pKa = 7.07 CCFSLRR133 pKa = 11.84 YY134 pKa = 9.86 SPFLKK139 pKa = 10.65 PLDD142 pKa = 3.48 RR143 pKa = 11.84 TKK145 pKa = 11.31 NKK147 pKa = 8.94 TVEE150 pKa = 3.8 ATKK153 pKa = 10.18 PDD155 pKa = 3.64 KK156 pKa = 10.84 KK157 pKa = 10.63 DD158 pKa = 3.43 GQGKK162 pKa = 9.53 SEE164 pKa = 4.82 GGGTDD169 pKa = 4.46 DD170 pKa = 4.03 NPKK173 pKa = 10.64 SGGEE177 pKa = 3.84 KK178 pKa = 10.46 SPVQPHH184 pKa = 5.81 VPHH187 pKa = 6.65 VDD189 pKa = 3.27 AEE191 pKa = 4.23 EE192 pKa = 3.98 QPIVDD197 pKa = 4.46 PAPGGTRR204 pKa = 11.84 MADD207 pKa = 3.03 TGSCYY212 pKa = 10.52 AAIVGQEE219 pKa = 4.13 DD220 pKa = 3.64 GHH222 pKa = 7.36 ARR224 pKa = 11.84 TVDD227 pKa = 3.58 GEE229 pKa = 4.02 PDD231 pKa = 3.08 IVGGVLLPISRR242 pKa = 11.84 EE243 pKa = 3.97 PNVPSDD249 pKa = 3.74 TINNVKK255 pKa = 10.23 KK256 pKa = 10.57 AIKK259 pKa = 9.85 EE260 pKa = 4.08 RR261 pKa = 11.84 IDD263 pKa = 3.96 EE264 pKa = 4.32 KK265 pKa = 11.07 QLPCNLTKK273 pKa = 10.91 DD274 pKa = 3.32 EE275 pKa = 4.07 TRR277 pKa = 11.84 RR278 pKa = 11.84 IGRR281 pKa = 11.84 LVSASLADD289 pKa = 3.52 NGPFARR295 pKa = 11.84 KK296 pKa = 9.94 RR297 pKa = 11.84 IRR299 pKa = 11.84 AWLAKK304 pKa = 10.34 NFDD307 pKa = 3.75 LAEE310 pKa = 4.34 IKK312 pKa = 10.23 SKK314 pKa = 10.14 KK315 pKa = 8.58 WSEE318 pKa = 3.79 EE319 pKa = 3.71 RR320 pKa = 11.84 LRR322 pKa = 11.84 AAVDD326 pKa = 3.43 KK327 pKa = 10.73 LYY329 pKa = 10.1 STSDD333 pKa = 3.37 PQFSYY338 pKa = 11.04 AAAVKK343 pKa = 10.05 AEE345 pKa = 4.0 QMPEE349 pKa = 3.89 GKK351 pKa = 9.34 PPRR354 pKa = 11.84 MLIADD359 pKa = 4.64 GDD361 pKa = 4.27 PGQVMALMTIACFEE375 pKa = 4.19 SLMYY379 pKa = 10.08 EE380 pKa = 3.99 WYY382 pKa = 9.45 EE383 pKa = 3.81 NLSIKK388 pKa = 10.0 HH389 pKa = 5.57 ASRR392 pKa = 11.84 RR393 pKa = 11.84 AAMKK397 pKa = 10.15 RR398 pKa = 11.84 VLNHH402 pKa = 6.28 LRR404 pKa = 11.84 QDD406 pKa = 3.93 SEE408 pKa = 4.75 CSFLEE413 pKa = 4.31 GDD415 pKa = 3.85 GSAWDD420 pKa = 4.02 TTCSHH425 pKa = 7.04 RR426 pKa = 11.84 VRR428 pKa = 11.84 SIVEE432 pKa = 3.79 NPVLKK437 pKa = 10.6 RR438 pKa = 11.84 ILEE441 pKa = 4.24 VARR444 pKa = 11.84 EE445 pKa = 3.91 QAVVPEE451 pKa = 4.27 QWLEE455 pKa = 3.84 AHH457 pKa = 6.59 DD458 pKa = 3.87 TSTSKK463 pKa = 10.68 KK464 pKa = 9.43 HH465 pKa = 6.34 LKK467 pKa = 10.49 LKK469 pKa = 9.31 LTEE472 pKa = 4.26 KK473 pKa = 9.57 GKK475 pKa = 10.69 KK476 pKa = 8.1 MVYY479 pKa = 9.81 EE480 pKa = 4.5 VIDD483 pKa = 3.81 SIRR486 pKa = 11.84 RR487 pKa = 11.84 SGHH490 pKa = 6.33 RR491 pKa = 11.84 GTSCLNWWINNVLWICALVQNPEE514 pKa = 3.8 KK515 pKa = 10.69 SISATATNFMDD526 pKa = 4.54 FWGEE530 pKa = 3.87 TITIKK535 pKa = 10.58 RR536 pKa = 11.84 AFEE539 pKa = 4.49 GDD541 pKa = 3.56 DD542 pKa = 3.4 SGLTVAPKK550 pKa = 10.07 MKK552 pKa = 10.15 DD553 pKa = 3.2 VKK555 pKa = 10.89 DD556 pKa = 3.55 PRR558 pKa = 11.84 FEE560 pKa = 4.04 HH561 pKa = 7.03 ALEE564 pKa = 4.24 FWHH567 pKa = 7.23 RR568 pKa = 11.84 AGFNMKK574 pKa = 9.63 IFLRR578 pKa = 11.84 HH579 pKa = 4.9 NVGLFVGTEE588 pKa = 3.64 IALDD592 pKa = 3.46 EE593 pKa = 4.89 HH594 pKa = 6.94 GPTGEE599 pKa = 4.12 YY600 pKa = 10.63 APEE603 pKa = 4.12 LKK605 pKa = 9.57 RR606 pKa = 11.84 TFEE609 pKa = 4.21 KK610 pKa = 10.96 AGISCSSLTKK620 pKa = 10.64 KK621 pKa = 10.45 LIKK624 pKa = 10.53 AGAAGNEE631 pKa = 4.13 GLHH634 pKa = 4.98 EE635 pKa = 4.29 VRR637 pKa = 11.84 RR638 pKa = 11.84 SLALAKK644 pKa = 10.36 AYY646 pKa = 10.29 DD647 pKa = 3.72 FAGIVPSVSRR657 pKa = 11.84 KK658 pKa = 9.66 YY659 pKa = 10.54 LLCAQGLDD667 pKa = 3.98 TMDD670 pKa = 5.08 HH671 pKa = 5.48 EE672 pKa = 5.05 LKK674 pKa = 10.09 MRR676 pKa = 11.84 TGEE679 pKa = 4.16 TTLEE683 pKa = 4.7 GIRR686 pKa = 11.84 DD687 pKa = 4.03 MIDD690 pKa = 3.12 AANSTCSPEE699 pKa = 5.46 DD700 pKa = 3.4 EE701 pKa = 4.79 DD702 pKa = 5.81 ALLEE706 pKa = 4.0 RR707 pKa = 11.84 LGRR710 pKa = 11.84 SISQTEE716 pKa = 3.53 RR717 pKa = 11.84 DD718 pKa = 3.79 AFEE721 pKa = 4.41 AYY723 pKa = 9.0 CWAWEE728 pKa = 4.16 KK729 pKa = 10.74 EE730 pKa = 4.09 ATSQCEE736 pKa = 3.84 EE737 pKa = 4.21 FAASLPASWRR747 pKa = 11.84 VV748 pKa = 3.01
Molecular weight: 83.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.367
IPC2_protein 6.389
IPC_protein 6.44
Toseland 6.561
ProMoST 6.883
Dawson 6.839
Bjellqvist 6.854
Wikipedia 6.839
Rodwell 6.839
Grimsley 6.678
Solomon 6.854
Lehninger 6.854
Nozaki 7.161
DTASelect 7.234
Thurlkill 7.278
EMBOSS 7.249
Sillero 7.263
Patrickios 4.52
IPC_peptide 6.868
IPC2_peptide 6.912
IPC2.peptide.svr19 6.814
Protein with the highest isoelectric point:
>tr|A0A1L3KL57|A0A1L3KL57_9VIRU Uncharacterized protein OS=Beihai weivirus-like virus 7 OX=1922754 PE=4 SV=1
MM1 pKa = 7.65 ARR3 pKa = 11.84 ARR5 pKa = 11.84 KK6 pKa = 9.9 NGTQARR12 pKa = 11.84 KK13 pKa = 9.64 RR14 pKa = 11.84 MPRR17 pKa = 11.84 RR18 pKa = 11.84 GRR20 pKa = 11.84 RR21 pKa = 11.84 SGAPAVQAQGTGTTVAVPFGSNGRR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 KK48 pKa = 10.5 SMAAMAMQGWNAFHH62 pKa = 7.57 PYY64 pKa = 9.88 HH65 pKa = 6.77 LPLPRR70 pKa = 11.84 AVGPYY75 pKa = 8.91 TVIRR79 pKa = 11.84 TSTLITNSHH88 pKa = 6.49 KK89 pKa = 10.5 FMQFGTFVDD98 pKa = 5.71 DD99 pKa = 3.79 NGFWTNVVGLGSVVSSDD116 pKa = 4.13 PINGGGNTALFTVPTPAVASLSGTGFTCVPASLSVQVMNNTALMQANGIFGGGVCHH172 pKa = 6.05 TQMSLGGRR180 pKa = 11.84 AEE182 pKa = 4.36 TYY184 pKa = 10.9 NDD186 pKa = 3.71 LSTEE190 pKa = 4.81 FISYY194 pKa = 7.47 MRR196 pKa = 11.84 PRR198 pKa = 11.84 LMSGGKK204 pKa = 9.21 LALKK208 pKa = 9.49 GVQMDD213 pKa = 4.59 SYY215 pKa = 9.61 PMNMSALANFEE226 pKa = 5.07 KK227 pKa = 9.59 ITKK230 pKa = 10.35 NIDD233 pKa = 3.11 HH234 pKa = 7.42 NITYY238 pKa = 10.41 SAGPYY243 pKa = 7.54 ATGMAPIIFVNQSEE257 pKa = 4.61 AEE259 pKa = 3.97 ITYY262 pKa = 8.35 LACIEE267 pKa = 3.87 WRR269 pKa = 11.84 VRR271 pKa = 11.84 FDD273 pKa = 2.86 IGSAAVASHH282 pKa = 5.33 VHH284 pKa = 6.23 HH285 pKa = 6.71 GVTPDD290 pKa = 3.89 SLWNDD295 pKa = 4.03 CVQTAVSLGHH305 pKa = 5.75 GVKK308 pKa = 10.39 EE309 pKa = 4.18 IADD312 pKa = 3.91 TVATVGTSINNVRR325 pKa = 11.84 AALFRR330 pKa = 11.84 ANPQPMLVDD339 pKa = 3.8
Molecular weight: 36.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.952
IPC2_protein 9.224
IPC_protein 9.37
Toseland 9.736
ProMoST 9.575
Dawson 10.028
Bjellqvist 9.78
Wikipedia 10.233
Rodwell 10.204
Grimsley 10.116
Solomon 10.058
Lehninger 10.014
Nozaki 9.853
DTASelect 9.75
Thurlkill 9.853
EMBOSS 10.175
Sillero 9.955
Patrickios 5.461
IPC_peptide 10.058
IPC2_peptide 8.756
IPC2.peptide.svr19 8.094
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1087
339
748
543.5
60.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.66 ± 0.474
1.84 ± 0.326
4.876 ± 0.805
6.348 ± 2.115
3.772 ± 0.177
7.544 ± 1.227
2.576 ± 0.039
4.416 ± 0.004
5.888 ± 1.596
7.452 ± 0.912
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.128 ± 0.786
4.048 ± 1.06
4.968 ± 0.169
2.944 ± 0.294
6.164 ± 0.13
7.268 ± 0.053
6.072 ± 1.08
6.716 ± 0.908
2.116 ± 0.462
2.208 ± 0.075
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here