Streptococcus satellite phage Javan455
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZNZ6|A0A4D5ZNZ6_9VIRU PriCT_1 domain-containing protein OS=Streptococcus satellite phage Javan455 OX=2558711 GN=JavanS455_0009 PE=4 SV=1
MM1 pKa = 7.72 AKK3 pKa = 10.2 NSPQDD8 pKa = 3.86 LINRR12 pKa = 11.84 EE13 pKa = 3.91 IFSTNLNMLMAKK25 pKa = 10.2 KK26 pKa = 10.1 NIKK29 pKa = 10.21 QIDD32 pKa = 3.46 IHH34 pKa = 6.66 NKK36 pKa = 9.74 LGIPKK41 pKa = 8.5 STITGYY47 pKa = 10.91 VKK49 pKa = 10.57 GRR51 pKa = 11.84 SLPTAGNVQKK61 pKa = 10.8 LADD64 pKa = 3.93 FFGVLKK70 pKa = 10.84 SDD72 pKa = 3.19 IDD74 pKa = 3.86 PRR76 pKa = 11.84 FDD78 pKa = 4.03 SNNIEE83 pKa = 4.29 TNSNIIPSTLQKK95 pKa = 9.47 VTSTLSQLEE104 pKa = 4.51 HH105 pKa = 6.54 KK106 pKa = 10.36 RR107 pKa = 11.84 QLNVLDD113 pKa = 3.87 YY114 pKa = 11.66 AEE116 pKa = 4.47 TQLEE120 pKa = 4.14 QQNTVEE126 pKa = 4.75 EE127 pKa = 4.39 PQATYY132 pKa = 8.87 YY133 pKa = 9.48 TYY135 pKa = 11.16 NYY137 pKa = 9.02 YY138 pKa = 11.1 DD139 pKa = 3.61 HH140 pKa = 7.34 AASAGTGQYY149 pKa = 11.03 LNDD152 pKa = 3.69 VQVEE156 pKa = 4.58 TIEE159 pKa = 5.25 LPVDD163 pKa = 3.45 YY164 pKa = 10.6 DD165 pKa = 3.94 ADD167 pKa = 3.98 FVIPVYY173 pKa = 10.34 GDD175 pKa = 3.3 SMEE178 pKa = 4.56 PEE180 pKa = 4.48 YY181 pKa = 11.08 HH182 pKa = 6.24 SGDD185 pKa = 3.53 YY186 pKa = 11.17 VFIKK190 pKa = 10.62 LSINLSDD197 pKa = 3.52 GDD199 pKa = 3.54 IGVFEE204 pKa = 4.94 YY205 pKa = 11.34 YY206 pKa = 10.64 GDD208 pKa = 4.18 AYY210 pKa = 9.96 IKK212 pKa = 10.44 QLVINDD218 pKa = 3.53 SGAFLHH224 pKa = 6.49 SLNDD228 pKa = 3.42 KK229 pKa = 10.89 YY230 pKa = 11.69 DD231 pKa = 5.24 DD232 pKa = 3.68 ILIDD236 pKa = 3.7 RR237 pKa = 11.84 DD238 pKa = 3.23 SDD240 pKa = 3.49 FRR242 pKa = 11.84 IIGEE246 pKa = 4.32 VIGSFTSKK254 pKa = 10.46 SS255 pKa = 3.33
Molecular weight: 28.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.421
IPC2_protein 4.482
IPC_protein 4.444
Toseland 4.266
ProMoST 4.584
Dawson 4.431
Bjellqvist 4.571
Wikipedia 4.342
Rodwell 4.279
Grimsley 4.177
Solomon 4.418
Lehninger 4.38
Nozaki 4.533
DTASelect 4.774
Thurlkill 4.291
EMBOSS 4.355
Sillero 4.571
Patrickios 3.478
IPC_peptide 4.418
IPC2_peptide 4.558
IPC2.peptide.svr19 4.5
Protein with the highest isoelectric point:
>tr|A0A4D5ZTS1|A0A4D5ZTS1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan455 OX=2558711 GN=JavanS455_0007 PE=4 SV=1
MM1 pKa = 7.9 KK2 pKa = 9.39 ITEE5 pKa = 4.25 HH6 pKa = 6.32 KK7 pKa = 9.64 KK8 pKa = 9.75 KK9 pKa = 10.59 NGTIVYY15 pKa = 9.02 RR16 pKa = 11.84 ASIYY20 pKa = 10.8 LGIDD24 pKa = 2.83 QMTGKK29 pKa = 10.05 RR30 pKa = 11.84 VKK32 pKa = 9.97 TSITGRR38 pKa = 11.84 TRR40 pKa = 11.84 KK41 pKa = 9.11 EE42 pKa = 3.7 VNQKK46 pKa = 10.3 AKK48 pKa = 10.27 HH49 pKa = 5.39 AQLDD53 pKa = 3.99 FLSNGSTIKK62 pKa = 10.43 RR63 pKa = 11.84 KK64 pKa = 9.88 VVIKK68 pKa = 8.87 TFKK71 pKa = 10.26 EE72 pKa = 4.46 LSHH75 pKa = 6.68 LWLEE79 pKa = 4.6 TYY81 pKa = 10.71 KK82 pKa = 10.43 LTVKK86 pKa = 10.0 PQTYY90 pKa = 9.55 DD91 pKa = 3.09 ATVTRR96 pKa = 11.84 LNRR99 pKa = 11.84 HH100 pKa = 5.38 IMPTLGNMKK109 pKa = 9.51 VDD111 pKa = 4.87 KK112 pKa = 9.89 ITASDD117 pKa = 3.12 IQMLINRR124 pKa = 11.84 LSKK127 pKa = 11.11 YY128 pKa = 8.49 YY129 pKa = 11.04 VNYY132 pKa = 8.12 TAVRR136 pKa = 11.84 SVIRR140 pKa = 11.84 KK141 pKa = 8.39 VLQQGVLLGLIDD153 pKa = 3.95 YY154 pKa = 10.62 NSARR158 pKa = 11.84 DD159 pKa = 3.44 IILPRR164 pKa = 11.84 KK165 pKa = 8.91 QPNAKK170 pKa = 9.88 KK171 pKa = 9.75 KK172 pKa = 10.82 VKK174 pKa = 10.53 FIDD177 pKa = 4.16 PSDD180 pKa = 3.48 LKK182 pKa = 11.33 SFLEE186 pKa = 4.04 HH187 pKa = 7.4 LEE189 pKa = 4.21 TSQHH193 pKa = 5.32 KK194 pKa = 10.23 RR195 pKa = 11.84 YY196 pKa = 10.13 NLYY199 pKa = 10.34 FDD201 pKa = 4.37 AVLYY205 pKa = 10.4 QLLLSTGLRR214 pKa = 11.84 IGEE217 pKa = 4.13 ACALEE222 pKa = 4.31 WGDD225 pKa = 3.94 IDD227 pKa = 5.91 LEE229 pKa = 4.47 NGTIAINKK237 pKa = 7.38 TYY239 pKa = 11.06 NKK241 pKa = 9.77 NLKK244 pKa = 9.76 FLSTAKK250 pKa = 8.49 TQSGNRR256 pKa = 11.84 VISVDD261 pKa = 3.32 KK262 pKa = 10.13 KK263 pKa = 7.7 TLRR266 pKa = 11.84 SLKK269 pKa = 9.82 LYY271 pKa = 9.48 QMRR274 pKa = 11.84 QRR276 pKa = 11.84 QLFNEE281 pKa = 3.92 VGARR285 pKa = 11.84 VSEE288 pKa = 4.58 VVFATPTRR296 pKa = 11.84 KK297 pKa = 9.82 YY298 pKa = 9.68 FNASVRR304 pKa = 11.84 QSALDD309 pKa = 3.79 TRR311 pKa = 11.84 CKK313 pKa = 9.91 EE314 pKa = 3.83 AGIEE318 pKa = 3.94 RR319 pKa = 11.84 FTFHH323 pKa = 7.65 AFRR326 pKa = 11.84 HH327 pKa = 4.69 THH329 pKa = 6.92 ASLLLNAGISYY340 pKa = 10.68 KK341 pKa = 10.23 EE342 pKa = 3.61 LQYY345 pKa = 11.51 RR346 pKa = 11.84 LGHH349 pKa = 6.41 ANISMTLDD357 pKa = 3.43 TYY359 pKa = 11.88 GHH361 pKa = 6.98 LSKK364 pKa = 11.01 DD365 pKa = 3.47 KK366 pKa = 10.63 EE367 pKa = 4.32 KK368 pKa = 11.01 EE369 pKa = 3.74 AVLYY373 pKa = 9.78 YY374 pKa = 10.38 EE375 pKa = 4.79 KK376 pKa = 11.4 AMNNLL381 pKa = 3.41
Molecular weight: 43.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.142
IPC2_protein 9.472
IPC_protein 9.443
Toseland 10.175
ProMoST 9.794
Dawson 10.335
Bjellqvist 9.984
Wikipedia 10.496
Rodwell 10.877
Grimsley 10.409
Solomon 10.365
Lehninger 10.321
Nozaki 10.145
DTASelect 9.984
Thurlkill 10.204
EMBOSS 10.555
Sillero 10.248
Patrickios 10.496
IPC_peptide 10.365
IPC2_peptide 8.507
IPC2.peptide.svr19 8.509
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
21
0
21
3360
40
562
160.0
18.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.685 ± 0.318
0.714 ± 0.278
5.982 ± 0.54
7.321 ± 0.648
4.018 ± 0.231
4.97 ± 0.462
1.905 ± 0.345
6.964 ± 0.461
8.958 ± 0.563
10.149 ± 0.548
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.054 ± 0.169
5.179 ± 0.323
3.482 ± 0.511
4.673 ± 0.226
5.179 ± 0.465
5.357 ± 0.375
6.458 ± 0.344
4.851 ± 0.349
1.012 ± 0.2
5.089 ± 0.462
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here