European mountain ash ringspot-associated virus (isolate Sorbus aucuparia) (EMARAV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Fimoviridae; Emaravirus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q6Q304|GLYC_EMARV Envelope glycoprotein OS=European mountain ash ringspot-associated virus (isolate Sorbus aucuparia) OX=1980426 PE=2 SV=2
MM1 pKa = 7.81EE2 pKa = 5.03SNKK5 pKa = 9.54MKK7 pKa = 10.46SITKK11 pKa = 7.34MTVNTLQMDD20 pKa = 3.94SCKK23 pKa = 10.46AAAKK27 pKa = 9.89RR28 pKa = 11.84FKK30 pKa = 10.61NATWEE35 pKa = 4.55LISNDD40 pKa = 3.36FNLDD44 pKa = 3.32IMNMFCILYY53 pKa = 7.82YY54 pKa = 10.82ACTSHH59 pKa = 6.79EE60 pKa = 4.41LKK62 pKa = 10.64LDD64 pKa = 3.5EE65 pKa = 6.11LVFQTAHH72 pKa = 5.75KK73 pKa = 10.66VIMNEE78 pKa = 3.69VSVSIGEE85 pKa = 3.84SRR87 pKa = 11.84MFIRR91 pKa = 11.84IYY93 pKa = 11.05VSFHH97 pKa = 5.62MLMGDD102 pKa = 3.67LNEE105 pKa = 4.71LLHH108 pKa = 7.47HH109 pKa = 7.0GISKK113 pKa = 10.95FEE115 pKa = 3.92GSPIKK120 pKa = 11.01DD121 pKa = 3.16MTSYY125 pKa = 11.07LIYY128 pKa = 10.66GEE130 pKa = 4.3EE131 pKa = 4.1YY132 pKa = 10.66VRR134 pKa = 11.84NLFQSINSTRR144 pKa = 11.84ATPVYY149 pKa = 10.26QLFITKK155 pKa = 9.63VPRR158 pKa = 11.84QFPAITHH165 pKa = 6.49GDD167 pKa = 3.43SEE169 pKa = 4.66YY170 pKa = 10.2TYY172 pKa = 10.24MLMVHH177 pKa = 6.7NYY179 pKa = 10.46LSTQSIAGVSHH190 pKa = 6.63TPSNSDD196 pKa = 2.9NYY198 pKa = 10.37VIRR201 pKa = 11.84TAEE204 pKa = 4.25EE205 pKa = 4.46YY206 pKa = 10.23VHH208 pKa = 6.48HH209 pKa = 7.39HH210 pKa = 5.58YY211 pKa = 10.93AQLDD215 pKa = 3.78RR216 pKa = 11.84QASDD220 pKa = 2.42NKK222 pKa = 9.52MRR224 pKa = 11.84PEE226 pKa = 4.54SSDD229 pKa = 3.14QMEE232 pKa = 3.96

Molecular weight: 26.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q0PI71|VG27_EMARV Uncharacterized 27 kDa protein OS=European mountain ash ringspot-associated virus (isolate Sorbus aucuparia) OX=1980426 PE=2 SV=1
MM1 pKa = 7.85PIIPKK6 pKa = 9.92PKK8 pKa = 9.1SQTKK12 pKa = 10.43GSVEE16 pKa = 4.04SSKK19 pKa = 11.06KK20 pKa = 8.23EE21 pKa = 3.78SRR23 pKa = 11.84VKK25 pKa = 10.41MEE27 pKa = 4.1TSDD30 pKa = 3.42AKK32 pKa = 11.33YY33 pKa = 9.73MVGNEE38 pKa = 3.69VKK40 pKa = 9.82TIKK43 pKa = 10.51FLDD46 pKa = 3.43MRR48 pKa = 11.84GNIATSARR56 pKa = 11.84NSLNISPGVFAVNPFLGEE74 pKa = 3.94TLAEE78 pKa = 4.06DD79 pKa = 3.73TFNILDD85 pKa = 3.72YY86 pKa = 11.35AGLGNVDD93 pKa = 3.3ACASHH98 pKa = 7.31LSRR101 pKa = 11.84SQEE104 pKa = 3.48LRR106 pKa = 11.84EE107 pKa = 3.97QVTEE111 pKa = 3.82KK112 pKa = 10.17TLRR115 pKa = 11.84EE116 pKa = 4.04VPISDD121 pKa = 3.79SYY123 pKa = 9.73VLKK126 pKa = 10.93VVSNLQATTVQNVVSFNKK144 pKa = 9.85ACAVMSFNILRR155 pKa = 11.84HH156 pKa = 5.15TTDD159 pKa = 4.83EE160 pKa = 4.27MYY162 pKa = 10.93DD163 pKa = 3.3WTKK166 pKa = 11.31NEE168 pKa = 3.97YY169 pKa = 10.4VSLGLKK175 pKa = 9.65EE176 pKa = 4.13KK177 pKa = 10.26AAKK180 pKa = 10.04VNPNIINRR188 pKa = 11.84LAGQINLSPQSPYY201 pKa = 11.06YY202 pKa = 10.5YY203 pKa = 10.31LVTPGYY209 pKa = 10.15EE210 pKa = 4.3FLYY213 pKa = 10.56DD214 pKa = 4.21AYY216 pKa = 9.59PAEE219 pKa = 4.78TIAMTLVKK227 pKa = 9.29MAYY230 pKa = 9.22RR231 pKa = 11.84KK232 pKa = 8.17TMNLPDD238 pKa = 3.74SMKK241 pKa = 11.04DD242 pKa = 3.19SDD244 pKa = 3.83ICSSLNAKK252 pKa = 9.2INKK255 pKa = 8.47RR256 pKa = 11.84HH257 pKa = 5.7NLAVNNIDD265 pKa = 6.07DD266 pKa = 4.72IIKK269 pKa = 10.5QIGKK273 pKa = 8.9KK274 pKa = 9.94HH275 pKa = 6.54IEE277 pKa = 4.03DD278 pKa = 3.87MYY280 pKa = 11.29NTLTQNIAMSGKK292 pKa = 9.14EE293 pKa = 3.82SRR295 pKa = 11.84NVEE298 pKa = 4.04TAQSFLALIEE308 pKa = 4.35SFKK311 pKa = 9.03TTTT314 pKa = 3.31

Molecular weight: 35.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3485

232

2293

871.3

100.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.644 ± 0.781

1.951 ± 0.517

6.284 ± 0.42

5.308 ± 0.397

4.735 ± 0.516

3.529 ± 0.132

2.669 ± 0.579

8.752 ± 0.566

8.063 ± 0.633

9.154 ± 0.808

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.869 ± 0.681

6.399 ± 0.389

3.156 ± 0.157

3.128 ± 0.248

3.357 ± 0.174

8.924 ± 0.23

6.428 ± 0.176

5.308 ± 0.882

0.459 ± 0.062

5.882 ± 0.338
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski