Synechococcus phage Syn30

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Leucotheavirus; Synechococcus virus Syn30

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 209 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4T072|M4T072_9CAUD DNA primase OS=Synechococcus phage Syn30 OX=536474 GN=CPRG_00110 PE=4 SV=1
MM1 pKa = 6.56TTAQKK6 pKa = 10.13FSSVIDD12 pKa = 3.65ILSDD16 pKa = 3.23AVDD19 pKa = 3.62RR20 pKa = 11.84QVTLDD25 pKa = 3.22IEE27 pKa = 4.36YY28 pKa = 9.22PIIYY32 pKa = 8.99NQVVKK37 pKa = 10.27FYY39 pKa = 10.15EE40 pKa = 4.12EE41 pKa = 4.25KK42 pKa = 10.99GVDD45 pKa = 3.88FYY47 pKa = 12.06GDD49 pKa = 3.03VDD51 pKa = 3.75EE52 pKa = 6.71DD53 pKa = 4.31YY54 pKa = 11.28DD55 pKa = 3.95ILLTKK60 pKa = 10.57LEE62 pKa = 3.93QDD64 pKa = 3.45LFYY67 pKa = 11.56YY68 pKa = 7.64EE69 pKa = 4.19TT70 pKa = 3.99

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4SLX4|M4SLX4_9CAUD Uncharacterized protein OS=Synechococcus phage Syn30 OX=536474 GN=CPRG_00166 PE=4 SV=1
MM1 pKa = 7.81ISRR4 pKa = 11.84RR5 pKa = 11.84CQTIRR10 pKa = 11.84KK11 pKa = 8.15AWRR14 pKa = 11.84VWAKK18 pKa = 11.06ALGAKK23 pKa = 10.03DD24 pKa = 3.2GRR26 pKa = 11.84TNRR29 pKa = 11.84EE30 pKa = 2.86ADD32 pKa = 3.36IIASIRR38 pKa = 11.84TLIFVSYY45 pKa = 9.58MVTNVAIVANAVRR58 pKa = 11.84HH59 pKa = 5.21WDD61 pKa = 3.59NNKK64 pKa = 9.43SVHH67 pKa = 6.7PIDD70 pKa = 3.89HH71 pKa = 6.58CRR73 pKa = 11.84SDD75 pKa = 3.71LLL77 pKa = 4.59

Molecular weight: 8.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

209

0

209

56296

49

5157

269.4

29.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.105 ± 0.213

0.876 ± 0.098

6.599 ± 0.144

5.928 ± 0.319

4.29 ± 0.109

7.898 ± 0.354

1.547 ± 0.108

6.279 ± 0.126

5.91 ± 0.382

7.157 ± 0.169

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.077 ± 0.178

5.988 ± 0.232

4.025 ± 0.169

3.688 ± 0.112

3.954 ± 0.188

7.048 ± 0.202

7.672 ± 0.542

6.595 ± 0.178

1.146 ± 0.081

4.219 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski