Dyella sp. AD56

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Rhodanobacteraceae; Dyella; unclassified Dyella

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4642 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N7QY61|A0A2N7QY61_9GAMM DNA polymerase IV OS=Dyella sp. AD56 OX=1528744 GN=dinB_1 PE=3 SV=1
MM1 pKa = 6.59NTIYY5 pKa = 11.08SKK7 pKa = 10.21IWNSALGAVVVASEE21 pKa = 4.45LARR24 pKa = 11.84MAGKK28 pKa = 10.17GRR30 pKa = 11.84QRR32 pKa = 11.84RR33 pKa = 11.84ARR35 pKa = 11.84TLTVIGVLVLSMAMANGVQAGVTLPIATPPTTTPASGTTTTVVDD79 pKa = 3.53PTGAVAISGPASGGTTTASTAGSNDD104 pKa = 3.13VAVGSGAVTLGSNGTAVGAGAQSQGDD130 pKa = 3.59NGTSLGAAANATATGANSTAVGTGAQSQGDD160 pKa = 3.58NGTAVGSAANAVGNAAVAVGANATATGMNSVALGAGAAAATDD202 pKa = 3.4SSMALGQGAQATGSAAMAMGAAAGATGSNSMALGQGAQATASNSIALGNSTVAASSSSIAMGSSASVTGVSSSVAIGPGASITNQTLAGTTAFGFYY298 pKa = 10.28RR299 pKa = 11.84NDD301 pKa = 2.85VAMGNGASLYY311 pKa = 10.62AATSSVAIGAQSFVGDD327 pKa = 4.1TSATPALVTSATAVGVGSSVQASYY351 pKa = 8.64GTAIGDD357 pKa = 3.62IASTSGTYY365 pKa = 8.42ATAVGYY371 pKa = 9.29GASSVGNTASAFGSGASAFGAGSTALGGNAQANGAGSIVIAGGGNGGGAYY421 pKa = 10.19SGPNSPNTVVIGNSAEE437 pKa = 4.13AVEE440 pKa = 4.66TTDD443 pKa = 4.49SNGNAQLVSNSVVIGSNAISYY464 pKa = 10.61GGLNGIAIGNTAGVAQGSIDD484 pKa = 4.15AIGIGTRR491 pKa = 11.84AIGYY495 pKa = 9.76GEE497 pKa = 4.54GSVAMGAGSYY507 pKa = 10.65AIGPGSTAVGGWLDD521 pKa = 3.4INGTGVVTGAGSGTTVSPGVGSSAFGAGAEE551 pKa = 4.43SIGDD555 pKa = 3.38FSVAMGAGATAAGSSTGYY573 pKa = 10.0PNANRR578 pKa = 11.84AGYY581 pKa = 8.5TNADD585 pKa = 3.37QTAGAIAIGYY595 pKa = 8.15EE596 pKa = 4.44SYY598 pKa = 11.27TNGDD602 pKa = 3.27GSVVIGRR609 pKa = 11.84NALANGSPDD618 pKa = 2.91GSLINGTAIGSQSVAWGTGAVAIGSSAVAAWTNDD652 pKa = 2.81QAMGTNAYY660 pKa = 10.09AGGGTGTNNMAIGNGATAGDD680 pKa = 4.02ALRR683 pKa = 11.84GTEE686 pKa = 4.07NNQNPPLPLPNISDD700 pKa = 3.72TVAIGASSSATANQAMALGVSASAAGVQAQAFGNNAIANGVQSMSNGYY748 pKa = 8.17MADD751 pKa = 3.54AEE753 pKa = 4.79GDD755 pKa = 3.48YY756 pKa = 11.76SMAQGSGAYY765 pKa = 9.62AAGTGDD771 pKa = 3.35VALGSGAVATGANGMGTAVGYY792 pKa = 9.63GATVTAQNAVAIGANSVADD811 pKa = 3.97RR812 pKa = 11.84DD813 pKa = 3.97NAVSVGAAGAEE824 pKa = 3.65RR825 pKa = 11.84QIINVAAGTQATDD838 pKa = 3.45AVNLSQLQAAGLVDD852 pKa = 4.19GNNNPLTAVTYY863 pKa = 11.11DD864 pKa = 3.6DD865 pKa = 3.71ATKK868 pKa = 10.86AGITLQGANGTQIHH882 pKa = 5.83NVAAGVAPMDD892 pKa = 4.43AVNMGQFTSATSTINGEE909 pKa = 4.16LSTLTTDD916 pKa = 4.69FTTLTQQLGAGGGGMPGTFAVNGQGGSMTNASANGANTVAIGNGAAVGTGVSGDD970 pKa = 3.16NGTAVGGGALAHH982 pKa = 6.57GPNDD986 pKa = 3.53TAFGGNAKK994 pKa = 10.0VNADD998 pKa = 2.9GSTAVGANTTIAVAATNAVAVGEE1021 pKa = 4.53SASVTAASGTAVGQGSSATATNTVALGQGSVANQANTVSVGTATNQRR1068 pKa = 11.84QIVNVAAGVNATDD1081 pKa = 3.66ATNVGQVSAQVTQALQTAKK1100 pKa = 9.77TYY1102 pKa = 10.68TDD1104 pKa = 3.47ASSQQAVQSANAYY1117 pKa = 6.89TNQAIANYY1125 pKa = 9.0DD1126 pKa = 3.61ANSGIGALRR1135 pKa = 11.84QEE1137 pKa = 4.9MNDD1140 pKa = 3.0QFNNVNQRR1148 pKa = 11.84LDD1150 pKa = 3.2RR1151 pKa = 11.84VGAMGSAMAQMTANTSNLAGDD1172 pKa = 3.56NRR1174 pKa = 11.84VGFGAGNYY1182 pKa = 8.99NGQGAFAVGYY1192 pKa = 9.39QRR1194 pKa = 11.84AFASSHH1200 pKa = 5.84ASVSVGASVSGSEE1213 pKa = 4.12TSVGVGGGFSWW1224 pKa = 3.94

Molecular weight: 114.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N7QVH0|A0A2N7QVH0_9GAMM 2-dehydropantoate 2-reductase OS=Dyella sp. AD56 OX=1528744 GN=panE PE=3 SV=1
MM1 pKa = 7.73AKK3 pKa = 6.84TTRR6 pKa = 11.84SATATKK12 pKa = 8.9PQKK15 pKa = 10.45GKK17 pKa = 9.35PAGKK21 pKa = 10.41VKK23 pKa = 10.43DD24 pKa = 3.74VKK26 pKa = 10.74AGKK29 pKa = 9.65KK30 pKa = 8.62VAAGRR35 pKa = 11.84SAVPTKK41 pKa = 10.45AAAVKK46 pKa = 10.4KK47 pKa = 9.45PAVKK51 pKa = 10.21NAPVKK56 pKa = 10.34KK57 pKa = 10.04AAAAKK62 pKa = 9.82KK63 pKa = 8.4PAAAKK68 pKa = 10.18VVAKK72 pKa = 10.76APAKK76 pKa = 10.38SVAKK80 pKa = 10.08KK81 pKa = 9.98AAPKK85 pKa = 10.18PPAKK89 pKa = 10.04KK90 pKa = 10.02ARR92 pKa = 11.84RR93 pKa = 11.84PRR95 pKa = 11.84NRR97 pKa = 11.84PSLRR101 pKa = 11.84KK102 pKa = 9.57LRR104 pKa = 11.84NRR106 pKa = 11.84SPRR109 pKa = 11.84KK110 pKa = 9.03FRR112 pKa = 11.84SPPRR116 pKa = 11.84RR117 pKa = 11.84SRR119 pKa = 11.84PRR121 pKa = 11.84RR122 pKa = 3.42

Molecular weight: 12.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4642

0

4642

1539333

30

4705

331.6

36.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.882 ± 0.045

0.831 ± 0.011

5.773 ± 0.03

4.917 ± 0.042

3.423 ± 0.023

8.42 ± 0.067

2.497 ± 0.018

4.365 ± 0.024

3.06 ± 0.032

10.471 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.365 ± 0.019

2.953 ± 0.039

5.171 ± 0.028

3.928 ± 0.028

6.772 ± 0.05

5.928 ± 0.034

5.521 ± 0.05

7.551 ± 0.031

1.57 ± 0.02

2.601 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski