Clostridium sp. OM02-18AC

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3013 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A396Q1B2|A0A396Q1B2_9CLOT Uncharacterized protein OS=Clostridium sp. OM02-18AC OX=2292311 GN=DXB18_14575 PE=4 SV=1
MM1 pKa = 7.21FTPKK5 pKa = 10.31NIQGALEE12 pKa = 4.07EE13 pKa = 5.47LYY15 pKa = 10.79DD16 pKa = 4.61LCDD19 pKa = 3.77PDD21 pKa = 5.15YY22 pKa = 10.8MVDD25 pKa = 3.24MLVNYY30 pKa = 10.23SEE32 pKa = 4.6EE33 pKa = 4.12FDD35 pKa = 5.3DD36 pKa = 5.77ISPALLAKK44 pKa = 10.25SFQKK48 pKa = 9.71NAEE51 pKa = 4.23MISEE55 pKa = 4.04YY56 pKa = 10.38RR57 pKa = 11.84VLSSAGEE64 pKa = 4.39GIDD67 pKa = 3.7YY68 pKa = 10.26QGKK71 pKa = 9.06VLLNSRR77 pKa = 11.84AVRR80 pKa = 11.84LLSYY84 pKa = 10.75VEE86 pKa = 5.09DD87 pKa = 3.48MSGDD91 pKa = 3.5EE92 pKa = 4.42KK93 pKa = 11.44VRR95 pKa = 11.84TIQSKK100 pKa = 7.86EE101 pKa = 3.44LWLAEE106 pKa = 4.02DD107 pKa = 3.22MTFYY111 pKa = 11.0VVSCMSTITMDD122 pKa = 3.5KK123 pKa = 11.1EE124 pKa = 3.65EE125 pKa = 4.92AICLNEE131 pKa = 3.85HH132 pKa = 6.46RR133 pKa = 11.84SVVTTVEE140 pKa = 4.18CEE142 pKa = 3.67DD143 pKa = 5.83DD144 pKa = 3.43IFFDD148 pKa = 3.85MGSLICEE155 pKa = 4.64LDD157 pKa = 4.38DD158 pKa = 3.63ICLFEE163 pKa = 5.52LLADD167 pKa = 4.03VDD169 pKa = 3.72ATIYY173 pKa = 10.61EE174 pKa = 4.33LL175 pKa = 4.55

Molecular weight: 19.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A396PVY3|A0A396PVY3_9CLOT Flavodoxin OS=Clostridium sp. OM02-18AC OX=2292311 GN=DXB18_13270 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.73MTFQPKK8 pKa = 8.63NRR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 8.89VHH16 pKa = 5.88GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.33GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3013

0

3013

978987

34

3927

324.9

36.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.211 ± 0.05

1.525 ± 0.016

5.465 ± 0.038

7.443 ± 0.052

3.966 ± 0.03

7.344 ± 0.039

1.901 ± 0.023

6.815 ± 0.041

6.321 ± 0.04

9.157 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.223 ± 0.024

4.01 ± 0.028

3.576 ± 0.021

3.531 ± 0.028

4.608 ± 0.038

5.733 ± 0.035

5.443 ± 0.034

6.95 ± 0.039

0.935 ± 0.015

3.843 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski