Potato virus M (strain Russian) (PVM)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus; Potato virus M

Average proteome isoelectric point is 7.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P17527|TGB2_PVMR Movement protein TGB2 OS=Potato virus M (strain Russian) OX=12168 GN=ORF3 PE=3 SV=1
MM1 pKa = 7.76DD2 pKa = 5.11VIVDD6 pKa = 4.1LLYY9 pKa = 10.26KK10 pKa = 10.66YY11 pKa = 10.62KK12 pKa = 10.7FEE14 pKa = 4.34RR15 pKa = 11.84LSNKK19 pKa = 8.88LVCPIVVHH27 pKa = 6.06CVPGAGKK34 pKa = 10.35SSLIRR39 pKa = 11.84EE40 pKa = 4.27LLEE43 pKa = 4.18LDD45 pKa = 4.3SRR47 pKa = 11.84FCAYY51 pKa = 8.73TAGVEE56 pKa = 4.09DD57 pKa = 4.13QPRR60 pKa = 11.84LSGNWIRR67 pKa = 11.84KK68 pKa = 7.95WSGQQPEE75 pKa = 4.58GKK77 pKa = 9.81FVVLDD82 pKa = 4.25EE83 pKa = 4.36YY84 pKa = 11.4TLLTEE89 pKa = 4.27VPPVFALFGDD99 pKa = 5.26PIQSNTSAVQRR110 pKa = 11.84ADD112 pKa = 3.85FVCSVSRR119 pKa = 11.84RR120 pKa = 11.84FGSATCGLLRR130 pKa = 11.84EE131 pKa = 4.65LGWNVRR137 pKa = 11.84SEE139 pKa = 4.22KK140 pKa = 10.75ADD142 pKa = 3.55LVQVSDD148 pKa = 4.91IYY150 pKa = 11.39TKK152 pKa = 10.99DD153 pKa = 3.38PLGKK157 pKa = 10.0VVFSEE162 pKa = 4.54EE163 pKa = 4.04EE164 pKa = 4.13VGCLLRR170 pKa = 11.84SHH172 pKa = 6.31GVEE175 pKa = 3.52ALSLQEE181 pKa = 3.62ITGQTFEE188 pKa = 4.22VVTFVTSEE196 pKa = 3.72NSPVINRR203 pKa = 11.84AAAYY207 pKa = 10.1QCMTRR212 pKa = 11.84HH213 pKa = 5.07RR214 pKa = 11.84TALHH218 pKa = 6.25ILCPDD223 pKa = 3.34ATYY226 pKa = 9.14TAAA229 pKa = 4.96

Molecular weight: 25.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P17965|RDRP_PVMR RNA replication polyprotein OS=Potato virus M (strain Russian) OX=12168 GN=ORF1 PE=3 SV=2
MM1 pKa = 7.83KK2 pKa = 10.35DD3 pKa = 3.23VTKK6 pKa = 10.78VALLIARR13 pKa = 11.84AMCASSGTFVFEE25 pKa = 4.09LAFSITEE32 pKa = 3.86YY33 pKa = 10.13TGRR36 pKa = 11.84PLGGGRR42 pKa = 11.84SKK44 pKa = 10.49YY45 pKa = 10.1ARR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84AISIARR56 pKa = 11.84CHH58 pKa = 5.64RR59 pKa = 11.84CYY61 pKa = 10.6RR62 pKa = 11.84LWPPTVFTTRR72 pKa = 11.84CDD74 pKa = 3.61NKK76 pKa = 10.2HH77 pKa = 5.87CVPGISYY84 pKa = 10.14NVRR87 pKa = 11.84VAQFIDD93 pKa = 3.44EE94 pKa = 4.6GVTEE98 pKa = 4.82VIPSVINKK106 pKa = 9.72RR107 pKa = 11.84EE108 pKa = 3.77

Molecular weight: 12.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2781

63

1968

463.5

52.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.983 ± 0.672

2.769 ± 0.501

4.495 ± 0.434

6.976 ± 0.993

4.746 ± 0.556

6.472 ± 0.536

2.445 ± 0.353

3.955 ± 0.551

5.502 ± 0.805

9.853 ± 0.736

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.805 ± 0.642

4.279 ± 0.649

4.063 ± 0.899

2.661 ± 0.292

6.94 ± 0.697

6.544 ± 0.611

4.423 ± 0.766

8.306 ± 0.87

1.474 ± 0.259

3.308 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski