Rice stripe virus (isolate T) (RSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Tenuivirus; Rice stripe tenuivirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q00847|MVP_RSVT Movement protein OS=Rice stripe virus (isolate T) OX=36394 GN=pc4 PE=1 SV=1
MM1 pKa = 7.46QDD3 pKa = 2.94VQRR6 pKa = 11.84TIEE9 pKa = 4.12VSVGPIVGLDD19 pKa = 3.21YY20 pKa = 11.19TLLYY24 pKa = 8.88DD25 pKa = 4.16TLPEE29 pKa = 4.2TVSDD33 pKa = 4.77NITLPDD39 pKa = 3.69LKK41 pKa = 10.92DD42 pKa = 3.57PEE44 pKa = 4.69RR45 pKa = 11.84VTEE48 pKa = 4.05DD49 pKa = 3.08TKK51 pKa = 11.68KK52 pKa = 10.9LILKK56 pKa = 8.1GCVYY60 pKa = 10.44IAYY63 pKa = 8.74HH64 pKa = 6.54HH65 pKa = 6.72PLEE68 pKa = 4.66TDD70 pKa = 3.34TLFIKK75 pKa = 8.66VHH77 pKa = 4.51KK78 pKa = 9.65HH79 pKa = 4.52IPEE82 pKa = 4.38FCHH85 pKa = 6.88SFLSHH90 pKa = 7.16LLGGEE95 pKa = 4.26DD96 pKa = 5.45DD97 pKa = 5.51DD98 pKa = 5.63NALIDD103 pKa = 3.49IGLFFNMLQPSLGGWITKK121 pKa = 10.07NFLRR125 pKa = 11.84HH126 pKa = 5.84PNRR129 pKa = 11.84MSKK132 pKa = 10.53DD133 pKa = 3.65QIKK136 pKa = 9.55MLLDD140 pKa = 3.72QIIKK144 pKa = 8.36MAKK147 pKa = 9.8AEE149 pKa = 4.11SSDD152 pKa = 3.35TEE154 pKa = 4.26EE155 pKa = 4.84YY156 pKa = 10.29EE157 pKa = 4.56KK158 pKa = 10.9VWKK161 pKa = 10.57KK162 pKa = 9.55MPTYY166 pKa = 10.04FEE168 pKa = 5.9SIIQPLLHH176 pKa = 6.1KK177 pKa = 9.26TT178 pKa = 3.39

Molecular weight: 20.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q85429|GLYC_RSVT Putative envelope glycoprotein OS=Rice stripe virus (isolate T) OX=36394 GN=pc2 PE=1 SV=1
MM1 pKa = 7.54ALSRR5 pKa = 11.84LLSTLKK11 pKa = 10.91SKK13 pKa = 10.61VLYY16 pKa = 10.3DD17 pKa = 3.98DD18 pKa = 5.58LSEE21 pKa = 4.24EE22 pKa = 4.11SQKK25 pKa = 10.89RR26 pKa = 11.84VDD28 pKa = 3.41NKK30 pKa = 10.31NRR32 pKa = 11.84KK33 pKa = 8.81SLALSKK39 pKa = 10.79RR40 pKa = 11.84PLNQGRR46 pKa = 11.84VTIDD50 pKa = 2.91QAATMLGLEE59 pKa = 4.61PFSFSDD65 pKa = 3.58VKK67 pKa = 10.79VNKK70 pKa = 9.95YY71 pKa = 11.5DD72 pKa = 3.54MFIAKK77 pKa = 9.29QDD79 pKa = 3.77YY80 pKa = 9.64SVKK83 pKa = 10.03AHH85 pKa = 6.9RR86 pKa = 11.84KK87 pKa = 6.93ATFNILVDD95 pKa = 4.67PYY97 pKa = 10.43WFHH100 pKa = 6.93QPLTHH105 pKa = 6.25YY106 pKa = 10.35PFFRR110 pKa = 11.84VEE112 pKa = 3.86TFAMVWIGIKK122 pKa = 10.34GRR124 pKa = 11.84ASGITTLRR132 pKa = 11.84IIDD135 pKa = 3.71KK136 pKa = 10.76SYY138 pKa = 11.43VNPSDD143 pKa = 3.51QVEE146 pKa = 4.33VEE148 pKa = 3.75VRR150 pKa = 11.84YY151 pKa = 9.28PISKK155 pKa = 9.91NFAVLGSLANFLALEE170 pKa = 4.71DD171 pKa = 3.66KK172 pKa = 11.15HH173 pKa = 7.22NLQVSVSVDD182 pKa = 3.14DD183 pKa = 5.47SSVQNCVISRR193 pKa = 11.84TLWFWGIEE201 pKa = 4.13RR202 pKa = 11.84TDD204 pKa = 4.19LPVSMKK210 pKa = 10.77TNDD213 pKa = 3.13TVMFEE218 pKa = 4.29FEE220 pKa = 4.26PLEE223 pKa = 4.52DD224 pKa = 3.42KK225 pKa = 10.99AINHH229 pKa = 6.85LSSFSNFTTNVVQKK243 pKa = 10.6AVGGAFTSKK252 pKa = 10.4SFPEE256 pKa = 4.34LDD258 pKa = 3.41TEE260 pKa = 4.65KK261 pKa = 10.87EE262 pKa = 4.1FGVVKK267 pKa = 10.17QPKK270 pKa = 9.4KK271 pKa = 10.54IPITKK276 pKa = 9.52KK277 pKa = 9.71SKK279 pKa = 10.72SEE281 pKa = 3.75VSVIMM286 pKa = 4.56

Molecular weight: 32.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

4949

178

2919

707.0

80.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.688 ± 0.538

1.879 ± 0.589

5.981 ± 0.335

6.87 ± 0.86

4.304 ± 0.239

4.344 ± 0.416

2.566 ± 0.438

7.072 ± 0.519

7.961 ± 0.348

9.578 ± 0.379

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.728 ± 0.147

4.607 ± 0.262

3.718 ± 0.686

3.011 ± 0.475

4.344 ± 0.562

8.487 ± 0.654

6.021 ± 0.549

6.668 ± 0.453

1.152 ± 0.169

4.021 ± 0.257
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski