Halomonas arcis
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3843 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H0AQP0|A0A1H0AQP0_9GAMM Elongation factor Tu OS=Halomonas arcis OX=416873 GN=tuf PE=3 SV=1
MM1 pKa = 6.69 QTLSLPRR8 pKa = 11.84 TLISAAVASAFALVAVNASADD29 pKa = 3.5 LSDD32 pKa = 4.96 RR33 pKa = 11.84 YY34 pKa = 10.77 VDD36 pKa = 3.75 NDD38 pKa = 3.65 GDD40 pKa = 4.0 LMADD44 pKa = 3.59 VPSDD48 pKa = 3.26 EE49 pKa = 5.06 SEE51 pKa = 4.06 WVDD54 pKa = 3.3 PDD56 pKa = 4.01 TLVFAYY62 pKa = 9.53 TPVEE66 pKa = 4.43 DD67 pKa = 3.79 PAVYY71 pKa = 10.81 ADD73 pKa = 3.38 VWSDD77 pKa = 3.94 FLDD80 pKa = 3.91 HH81 pKa = 7.29 LSDD84 pKa = 3.44 ATGKK88 pKa = 9.43 EE89 pKa = 4.18 VQFFPVQSNAAQQEE103 pKa = 4.14 ALRR106 pKa = 11.84 AGRR109 pKa = 11.84 LHH111 pKa = 6.08 VAGFNTGGVPVAVNCGGFRR130 pKa = 11.84 PFAMMAAEE138 pKa = 5.1 DD139 pKa = 4.3 GSFGYY144 pKa = 10.59 EE145 pKa = 3.89 MEE147 pKa = 5.14 IITYY151 pKa = 8.53 PGSGIEE157 pKa = 4.16 SVEE160 pKa = 4.06 DD161 pKa = 3.75 LEE163 pKa = 5.04 GSQLAFTSEE172 pKa = 4.03 TSNSGFRR179 pKa = 11.84 APSALLRR186 pKa = 11.84 SEE188 pKa = 4.38 YY189 pKa = 11.0 GMEE192 pKa = 4.11 AGEE195 pKa = 4.35 DD196 pKa = 3.81 FEE198 pKa = 4.97 TAFSGSHH205 pKa = 6.62 DD206 pKa = 3.71 NSVLGVVNEE215 pKa = 4.61 DD216 pKa = 3.5 YY217 pKa = 10.73 EE218 pKa = 4.3 AAAIANSVARR228 pKa = 11.84 RR229 pKa = 11.84 MLARR233 pKa = 11.84 GVVEE237 pKa = 4.09 EE238 pKa = 4.16 GDD240 pKa = 3.87 YY241 pKa = 11.15 EE242 pKa = 4.57 VVYY245 pKa = 10.11 TSQTFPTTAYY255 pKa = 10.47 GLAHH259 pKa = 6.6 NLDD262 pKa = 4.04 PEE264 pKa = 4.32 LAEE267 pKa = 5.5 DD268 pKa = 3.5 IQDD271 pKa = 3.89 AFFSYY276 pKa = 10.36 DD277 pKa = 3.06 WEE279 pKa = 4.54 GTALEE284 pKa = 4.51 EE285 pKa = 4.03 EE286 pKa = 4.67 FANSGEE292 pKa = 4.1 AQFIPITYY300 pKa = 8.74 QEE302 pKa = 3.9 NWEE305 pKa = 4.45 VIRR308 pKa = 11.84 TIADD312 pKa = 3.65 ANGVTYY318 pKa = 10.74 DD319 pKa = 3.98 CDD321 pKa = 3.29
Molecular weight: 34.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.719
IPC_protein 3.719
Toseland 3.503
ProMoST 3.834
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.592
Rodwell 3.541
Grimsley 3.414
Solomon 3.681
Lehninger 3.643
Nozaki 3.795
DTASelect 3.999
Thurlkill 3.541
EMBOSS 3.605
Sillero 3.834
Patrickios 0.922
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.751
Protein with the highest isoelectric point:
>tr|A0A1H0IX58|A0A1H0IX58_9GAMM NAD(P)-dependent dehydrogenase short-chain alcohol dehydrogenase family OS=Halomonas arcis OX=416873 GN=SAMN04487951_12235 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.04 RR14 pKa = 11.84 AHH16 pKa = 6.12 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.19 NGRR28 pKa = 11.84 AIIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.93 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3843
0
3843
1237372
39
8954
322.0
35.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.665 ± 0.041
0.928 ± 0.015
5.781 ± 0.053
6.188 ± 0.036
3.563 ± 0.028
7.723 ± 0.049
2.456 ± 0.023
5.056 ± 0.031
3.009 ± 0.031
11.259 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.632 ± 0.023
3.017 ± 0.027
4.751 ± 0.031
4.366 ± 0.035
6.37 ± 0.042
5.897 ± 0.034
5.244 ± 0.028
7.136 ± 0.031
1.472 ± 0.017
2.487 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here