Hubei sobemo-like virus 27

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KF61|A0A1L3KF61_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 27 OX=1923213 PE=4 SV=1
MM1 pKa = 5.74QTAVAKK7 pKa = 10.69HH8 pKa = 5.28EE9 pKa = 4.12AARR12 pKa = 11.84WDD14 pKa = 3.58IPDD17 pKa = 4.34DD18 pKa = 3.92FLEE21 pKa = 4.48EE22 pKa = 4.1THH24 pKa = 6.73FLRR27 pKa = 11.84AVEE30 pKa = 3.96EE31 pKa = 4.05VDD33 pKa = 3.77YY34 pKa = 10.72KK35 pKa = 11.22SSPGYY40 pKa = 9.81PYY42 pKa = 10.02MVRR45 pKa = 11.84NPSNSDD51 pKa = 3.09FFGVVDD57 pKa = 5.07GVANPARR64 pKa = 11.84LKK66 pKa = 10.01EE67 pKa = 3.65IWEE70 pKa = 4.03IVQIRR75 pKa = 11.84LHH77 pKa = 6.44KK78 pKa = 10.59LLLGEE83 pKa = 5.18DD84 pKa = 3.51DD85 pKa = 4.24CDD87 pKa = 4.42YY88 pKa = 11.35IRR90 pKa = 11.84LFIKK94 pKa = 10.68AEE96 pKa = 4.37PIKK99 pKa = 10.3EE100 pKa = 4.06KK101 pKa = 10.6KK102 pKa = 10.45LRR104 pKa = 11.84DD105 pKa = 3.35HH106 pKa = 7.54KK107 pKa = 11.15YY108 pKa = 9.69RR109 pKa = 11.84LISSVSVIDD118 pKa = 4.29QIIDD122 pKa = 3.27HH123 pKa = 6.32MLFDD127 pKa = 4.58PMNHH131 pKa = 6.29RR132 pKa = 11.84LYY134 pKa = 10.87DD135 pKa = 3.45NWHH138 pKa = 5.6MVPSKK143 pKa = 10.96VGWSPYY149 pKa = 9.14KK150 pKa = 10.42GGWRR154 pKa = 11.84IFPTQPLLAIDD165 pKa = 3.82KK166 pKa = 10.62SSWDD170 pKa = 3.21WTVCGWLIEE179 pKa = 4.27QIVQFRR185 pKa = 11.84IMLCNNINDD194 pKa = 3.72SWIKK198 pKa = 10.57LATQRR203 pKa = 11.84YY204 pKa = 8.12KK205 pKa = 11.02CLFLNPLFLTSGGLVLKK222 pKa = 10.48QKK224 pKa = 10.16EE225 pKa = 4.19AGVMKK230 pKa = 10.08SGCVNTIADD239 pKa = 3.89NSIMQLLLHH248 pKa = 6.37LRR250 pKa = 11.84VSLEE254 pKa = 3.72RR255 pKa = 11.84GGDD258 pKa = 3.48VDD260 pKa = 4.12GLFYY264 pKa = 10.89TMGDD268 pKa = 3.28DD269 pKa = 4.12TVQDD273 pKa = 4.14EE274 pKa = 4.71PNDD277 pKa = 3.72LDD279 pKa = 5.68DD280 pKa = 4.35YY281 pKa = 11.48LSRR284 pKa = 11.84LNQFSIVKK292 pKa = 9.54QATNRR297 pKa = 11.84TEE299 pKa = 3.77FAGMHH304 pKa = 6.09FNYY307 pKa = 10.44SQVEE311 pKa = 3.7PMYY314 pKa = 10.62GGKK317 pKa = 9.13HH318 pKa = 5.48AFNLLHH324 pKa = 7.18LDD326 pKa = 3.86PEE328 pKa = 4.45NEE330 pKa = 4.06EE331 pKa = 4.47QVCSSYY337 pKa = 10.73MLLYY341 pKa = 10.32HH342 pKa = 6.92RR343 pKa = 11.84SKK345 pKa = 10.63FRR347 pKa = 11.84GWFEE351 pKa = 4.62SLFEE355 pKa = 4.32AMNIPVPHH363 pKa = 6.67RR364 pKa = 11.84LVRR367 pKa = 11.84DD368 pKa = 4.0HH369 pKa = 7.28IFDD372 pKa = 3.55NSS374 pKa = 3.16

Molecular weight: 43.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KF61|A0A1L3KF61_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 27 OX=1923213 PE=4 SV=1
MM1 pKa = 7.41NPIILEE7 pKa = 4.07NEE9 pKa = 3.97EE10 pKa = 4.02TQEE13 pKa = 3.94NVLLEE18 pKa = 4.14RR19 pKa = 11.84TPQRR23 pKa = 11.84SFINRR28 pKa = 11.84IFWKK32 pKa = 9.92VVVVFATLLSLTALIPLLWLVIRR55 pKa = 11.84ALTYY59 pKa = 9.03RR60 pKa = 11.84LYY62 pKa = 11.12VAVEE66 pKa = 4.11YY67 pKa = 10.7QVVTQYY73 pKa = 10.51EE74 pKa = 4.24ALWRR78 pKa = 11.84FLAGLGQDD86 pKa = 4.23PPPEE90 pKa = 4.11PSVIEE95 pKa = 4.08KK96 pKa = 10.63VADD99 pKa = 3.44HH100 pKa = 5.73VTDD103 pKa = 4.26MIRR106 pKa = 11.84RR107 pKa = 11.84IDD109 pKa = 3.72SNIIAIGVIGIITFLAVVVSTFFVLRR135 pKa = 11.84SLRR138 pKa = 11.84KK139 pKa = 7.52ISYY142 pKa = 9.52RR143 pKa = 11.84IRR145 pKa = 11.84GIQFEE150 pKa = 4.11ALRR153 pKa = 11.84PGSTYY158 pKa = 10.06TKK160 pKa = 10.61EE161 pKa = 4.19EE162 pKa = 3.75IPNFQVRR169 pKa = 11.84VGQPGLFSASHH180 pKa = 5.28VGYY183 pKa = 10.47AVRR186 pKa = 11.84IGPNYY191 pKa = 8.69MAVPHH196 pKa = 5.8HH197 pKa = 6.62VIRR200 pKa = 11.84GQSTVLLTGPKK211 pKa = 9.54GRR213 pKa = 11.84CIVDD217 pKa = 3.4VVATQSRR224 pKa = 11.84MYY226 pKa = 10.59TDD228 pKa = 5.63LSYY231 pKa = 11.17CWVDD235 pKa = 3.22EE236 pKa = 4.56RR237 pKa = 11.84VWTTLGISIAKK248 pKa = 7.8LQPYY252 pKa = 7.64TSRR255 pKa = 11.84TVVRR259 pKa = 11.84CYY261 pKa = 10.84GQLGYY266 pKa = 9.48TSGSIYY272 pKa = 9.33KK273 pKa = 9.6TSLSPGIIAYY283 pKa = 8.99EE284 pKa = 4.18GSTEE288 pKa = 4.02NGTSGAAYY296 pKa = 9.91VLAGKK301 pKa = 10.17VLGLHH306 pKa = 6.15SAHH309 pKa = 6.31NPSSNMNFGVSSNMLLTEE327 pKa = 4.16YY328 pKa = 11.26AMLMKK333 pKa = 10.53PEE335 pKa = 4.21GKK337 pKa = 8.3KK338 pKa = 9.0TSATLDD344 pKa = 3.8HH345 pKa = 6.39YY346 pKa = 10.14TGDD349 pKa = 3.6APVTNVGKK357 pKa = 10.02GWSISEE363 pKa = 4.07SMAKK367 pKa = 9.9KK368 pKa = 8.23EE369 pKa = 4.52QEE371 pKa = 3.98WNDD374 pKa = 3.41VKK376 pKa = 11.15LKK378 pKa = 10.78NLATAAYY385 pKa = 9.32SHH387 pKa = 7.48DD388 pKa = 3.4WTNPPEE394 pKa = 4.3IDD396 pKa = 3.66YY397 pKa = 10.81NANLDD402 pKa = 3.55WGSDD406 pKa = 3.71DD407 pKa = 4.43EE408 pKa = 4.93EE409 pKa = 4.39SAKK412 pKa = 10.54VKK414 pKa = 10.67RR415 pKa = 11.84PVFQAGAFVPATVTLQHH432 pKa = 6.05QGAEE436 pKa = 4.16GEE438 pKa = 4.34MVEE441 pKa = 4.26TGLMNVNLVEE451 pKa = 4.21MVGALSKK458 pKa = 10.56RR459 pKa = 11.84VEE461 pKa = 4.21EE462 pKa = 4.45LEE464 pKa = 4.34KK465 pKa = 10.52FCASITEE472 pKa = 4.16PVQRR476 pKa = 11.84LIEE479 pKa = 4.51DD480 pKa = 3.22VDD482 pKa = 3.63GLKK485 pKa = 10.3NRR487 pKa = 11.84KK488 pKa = 8.45VVNATAKK495 pKa = 10.34AVAKK499 pKa = 7.17VTCEE503 pKa = 3.98CGVEE507 pKa = 4.16VAEE510 pKa = 5.19GKK512 pKa = 10.46LEE514 pKa = 3.84NHH516 pKa = 7.05ILNAHH521 pKa = 7.08PKK523 pKa = 9.77PIVQMACPDD532 pKa = 3.72CHH534 pKa = 8.13VMVAAFKK541 pKa = 10.14MDD543 pKa = 3.2AHH545 pKa = 7.23IKK547 pKa = 9.85NSHH550 pKa = 6.16PPSTKK555 pKa = 10.02VKK557 pKa = 10.55CSFCEE562 pKa = 4.13IQCKK566 pKa = 10.29DD567 pKa = 3.35SNRR570 pKa = 11.84LANHH574 pKa = 6.75IANVHH579 pKa = 5.46RR580 pKa = 11.84VMEE583 pKa = 4.42VKK585 pKa = 10.42PEE587 pKa = 4.04SAFSSDD593 pKa = 3.05EE594 pKa = 4.3TIKK597 pKa = 10.97VATRR601 pKa = 11.84PFLGRR606 pKa = 11.84SQRR609 pKa = 11.84PRR611 pKa = 11.84SNSPRR616 pKa = 11.84MRR618 pKa = 11.84PQQLSRR624 pKa = 11.84TSNVSTKK631 pKa = 10.03SQNSPSLEE639 pKa = 3.95AVLSLMTASLQNIEE653 pKa = 4.45KK654 pKa = 10.24RR655 pKa = 11.84LNAKK659 pKa = 10.01PPGTDD664 pKa = 3.35GQSSGIGPNN673 pKa = 3.15

Molecular weight: 74.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1047

374

673

523.5

58.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.495 ± 1.128

1.624 ± 0.01

4.871 ± 1.467

6.017 ± 0.205

3.534 ± 0.787

5.922 ± 0.292

2.865 ± 0.447

5.922 ± 0.116

5.349 ± 0.001

8.883 ± 0.786

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.961 ± 0.262

5.062 ± 0.009

5.158 ± 0.312

3.725 ± 0.009

5.253 ± 0.048

7.545 ± 0.574

5.158 ± 1.128

8.596 ± 0.973

1.815 ± 0.437

3.247 ± 0.252
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski