Apple stem pitting virus (isolate PA66) (ASPV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Foveavirus; Apple stem pitting virus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q64966|CAPSD_ASPVP Capsid protein OS=Apple stem pitting virus (isolate PA66) OX=651356 GN=ORF5 PE=4 SV=1
MM1 pKa = 7.47FPRR4 pKa = 11.84SGLGLAVAAAVVAYY18 pKa = 9.96LVLLLAQQLYY28 pKa = 9.26MSNSSQCTIVITGEE42 pKa = 4.12SVSVVGCVYY51 pKa = 10.74SEE53 pKa = 4.14AFIEE57 pKa = 4.21LVKK60 pKa = 10.72GLKK63 pKa = 9.64PYY65 pKa = 9.38YY66 pKa = 10.08HH67 pKa = 7.57PLGG70 pKa = 4.07

Molecular weight: 7.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q64965|TGB3_ASPVP Movement protein TGBp3 OS=Apple stem pitting virus (isolate PA66) OX=651356 GN=ORF4 PE=3 SV=1
MM1 pKa = 7.86PFAQPPDD8 pKa = 3.53YY9 pKa = 10.39SKK11 pKa = 11.3SVFPIAVGIAVAVVLFTLTRR31 pKa = 11.84STLPQVGDD39 pKa = 3.89NIHH42 pKa = 6.29NLPHH46 pKa = 6.56GGNYY50 pKa = 9.26QDD52 pKa = 3.31GTKK55 pKa = 10.06RR56 pKa = 11.84ISYY59 pKa = 9.2CGPRR63 pKa = 11.84DD64 pKa = 3.81SFPSSSLISSGTPMIIGIIIFLIFAIYY91 pKa = 10.37VSEE94 pKa = 3.81KK95 pKa = 8.64WSRR98 pKa = 11.84SGSRR102 pKa = 11.84RR103 pKa = 11.84CSCCVPGAPACTATVHH119 pKa = 5.72EE120 pKa = 4.63

Molecular weight: 12.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3010

70

2183

602.0

67.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.777 ± 1.537

2.392 ± 0.466

5.017 ± 1.369

6.412 ± 0.725

5.947 ± 0.953

6.611 ± 1.119

2.924 ± 0.38

6.246 ± 0.772

5.88 ± 1.385

9.535 ± 1.526

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.993 ± 0.287

4.385 ± 0.46

4.718 ± 1.3

3.09 ± 0.738

5.316 ± 0.447

8.339 ± 0.803

4.817 ± 0.69

6.213 ± 1.203

0.897 ± 0.228

2.492 ± 0.616
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski