Pedobacter nyackensis
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4961 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W2D255|A0A1W2D255_9SPHI Uncharacterized protein OS=Pedobacter nyackensis OX=475255 GN=SAMN04488101_105192 PE=4 SV=1
MM1 pKa = 7.69 EE2 pKa = 5.66 DD3 pKa = 3.77 AIKK6 pKa = 10.93 GLVPHH11 pKa = 6.37 VLSFIVSEE19 pKa = 4.16 FCKK22 pKa = 10.69 YY23 pKa = 10.7 GFLIAYY29 pKa = 7.55 EE30 pKa = 4.59 KK31 pKa = 10.92 DD32 pKa = 3.32 LSDD35 pKa = 3.87 LKK37 pKa = 11.43 GLIEE41 pKa = 4.91 PDD43 pKa = 3.85 SISAEE48 pKa = 3.91 DD49 pKa = 4.2 FEE51 pKa = 5.61 LLEE54 pKa = 4.65 AVDD57 pKa = 4.69 DD58 pKa = 4.29 PVVQLLLRR66 pKa = 11.84 SIDD69 pKa = 3.59 KK70 pKa = 10.54 VVNCSKK76 pKa = 10.07 TYY78 pKa = 10.86 FMINNLDD85 pKa = 3.7 EE86 pKa = 4.8 YY87 pKa = 10.83 EE88 pKa = 4.16 VMEE91 pKa = 4.29 NEE93 pKa = 5.15 EE94 pKa = 4.26 YY95 pKa = 10.89 NQLASDD101 pKa = 3.89 NYY103 pKa = 10.3 YY104 pKa = 10.19 IYY106 pKa = 10.43 IIDD109 pKa = 4.14 WEE111 pKa = 4.35 NKK113 pKa = 8.15 TYY115 pKa = 10.97 EE116 pKa = 4.49 DD117 pKa = 5.3 LLINLNAVYY126 pKa = 7.93 FTIARR131 pKa = 11.84 LLYY134 pKa = 10.32 HH135 pKa = 7.32 CATQIRR141 pKa = 11.84 RR142 pKa = 11.84 NEE144 pKa = 3.76 IEE146 pKa = 4.95 LPDD149 pKa = 3.98 EE150 pKa = 4.82 FYY152 pKa = 11.27 DD153 pKa = 5.92 DD154 pKa = 4.85 EE155 pKa = 5.7 FLDD158 pKa = 4.65 HH159 pKa = 6.99 YY160 pKa = 11.73 NEE162 pKa = 4.12 LLDD165 pKa = 5.37 RR166 pKa = 11.84 KK167 pKa = 9.99 LQSEE171 pKa = 4.69 DD172 pKa = 3.4 KK173 pKa = 10.88 NVALLYY179 pKa = 10.79 DD180 pKa = 5.11 LIADD184 pKa = 4.84 LNLDD188 pKa = 4.77 LYY190 pKa = 11.28 DD191 pKa = 4.69 IDD193 pKa = 4.04 QLSS196 pKa = 3.06
Molecular weight: 23.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.78
IPC2_protein 3.935
IPC_protein 3.923
Toseland 3.719
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.808
Rodwell 3.745
Grimsley 3.617
Solomon 3.897
Lehninger 3.846
Nozaki 4.012
DTASelect 4.215
Thurlkill 3.757
EMBOSS 3.821
Sillero 4.037
Patrickios 0.769
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.929
Protein with the highest isoelectric point:
>tr|A0A1W2F517|A0A1W2F517_9SPHI AraC-type DNA-binding protein OS=Pedobacter nyackensis OX=475255 GN=SAMN04488101_12137 PE=4 SV=1
MM1 pKa = 7.35 VDD3 pKa = 3.21 KK4 pKa = 10.44 FFSISYY10 pKa = 8.96 SSCSKK15 pKa = 9.88 EE16 pKa = 3.76 RR17 pKa = 11.84 RR18 pKa = 11.84 SNAFLIATRR27 pKa = 11.84 GKK29 pKa = 10.05 RR30 pKa = 11.84 VVNHH34 pKa = 6.61 LIGRR38 pKa = 11.84 ITAGMGHH45 pKa = 7.03 HH46 pKa = 7.42 DD47 pKa = 3.92 FLFFDD52 pKa = 3.67 RR53 pKa = 11.84 RR54 pKa = 11.84 KK55 pKa = 10.09 NFPIKK60 pKa = 10.06 VRR62 pKa = 11.84 KK63 pKa = 9.43 PNDD66 pKa = 3.11 TDD68 pKa = 3.18
Molecular weight: 7.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.314
IPC2_protein 9.648
IPC_protein 10.262
Toseland 10.906
ProMoST 10.54
Dawson 10.965
Bjellqvist 10.643
Wikipedia 11.155
Rodwell 11.257
Grimsley 10.979
Solomon 11.111
Lehninger 11.082
Nozaki 10.891
DTASelect 10.628
Thurlkill 10.891
EMBOSS 11.301
Sillero 10.891
Patrickios 11.023
IPC_peptide 11.111
IPC2_peptide 9.502
IPC2.peptide.svr19 8.746
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4961
0
4961
1862186
39
7858
375.4
42.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.401 ± 0.032
0.743 ± 0.01
5.306 ± 0.02
5.596 ± 0.037
4.847 ± 0.022
7.01 ± 0.04
1.686 ± 0.019
7.174 ± 0.033
7.34 ± 0.039
9.385 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.299 ± 0.017
5.966 ± 0.035
3.771 ± 0.021
3.541 ± 0.018
3.771 ± 0.02
6.527 ± 0.03
5.887 ± 0.048
6.349 ± 0.021
1.204 ± 0.012
4.199 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here