Beihai narna-like virus 23

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KIB7|A0A1L3KIB7_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 23 OX=1922451 PE=4 SV=1
MM1 pKa = 8.38DD2 pKa = 4.63YY3 pKa = 11.45LNMGFTFRR11 pKa = 11.84DD12 pKa = 3.66DD13 pKa = 4.03RR14 pKa = 11.84SIPDD18 pKa = 3.97DD19 pKa = 4.09PNEE22 pKa = 3.96GFTRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84FIEE31 pKa = 4.1EE32 pKa = 4.22LVLSIQTGSPPPAALEE48 pKa = 4.72DD49 pKa = 3.88GLHH52 pKa = 6.16RR53 pKa = 11.84FVEE56 pKa = 4.32ADD58 pKa = 3.19SYY60 pKa = 11.42LLHH63 pKa = 6.44VLSKK67 pKa = 11.05SQDD70 pKa = 3.31LPNILFLVSRR80 pKa = 11.84DD81 pKa = 3.1KK82 pKa = 11.18RR83 pKa = 11.84LALRR87 pKa = 11.84MRR89 pKa = 11.84DD90 pKa = 3.4VARR93 pKa = 11.84GVVTRR98 pKa = 11.84EE99 pKa = 3.21RR100 pKa = 11.84GYY102 pKa = 9.8NASRR106 pKa = 11.84DD107 pKa = 3.71VVIYY111 pKa = 9.79MVDD114 pKa = 3.24PADD117 pKa = 3.87FRR119 pKa = 11.84FGYY122 pKa = 9.14MNEE125 pKa = 5.05HH126 pKa = 6.29IAHH129 pKa = 5.88WTGGYY134 pKa = 9.17PDD136 pKa = 3.84SKK138 pKa = 9.97VPYY141 pKa = 9.91RR142 pKa = 11.84EE143 pKa = 3.97FVDD146 pKa = 4.7FGQVNFLAFTKK157 pKa = 10.72DD158 pKa = 3.63PIDD161 pKa = 3.77PFYY164 pKa = 9.96EE165 pKa = 4.05TEE167 pKa = 4.67RR168 pKa = 11.84IHH170 pKa = 7.28AVPCKK175 pKa = 10.08IRR177 pKa = 11.84GDD179 pKa = 3.88LVPGVYY185 pKa = 9.94HH186 pKa = 6.06SEE188 pKa = 4.18RR189 pKa = 11.84VCADD193 pKa = 3.61YY194 pKa = 11.28QDD196 pKa = 3.76TEE198 pKa = 4.65YY199 pKa = 10.68PFLSLTTQPLEE210 pKa = 4.12TILRR214 pKa = 11.84DD215 pKa = 3.55GVRR218 pKa = 11.84YY219 pKa = 8.11PHH221 pKa = 6.84IPSFARR227 pKa = 11.84DD228 pKa = 3.48LSAPTTWW235 pKa = 3.26

Molecular weight: 27.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KIB7|A0A1L3KIB7_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 23 OX=1922451 PE=4 SV=1
MM1 pKa = 7.48SGHH4 pKa = 6.01FQRR7 pKa = 11.84ALSKK11 pKa = 10.61LCRR14 pKa = 11.84SYY16 pKa = 11.33DD17 pKa = 3.32YY18 pKa = 10.37RR19 pKa = 11.84TIQRR23 pKa = 11.84WWYY26 pKa = 7.85TVDD29 pKa = 4.18GLVLPLLLTHH39 pKa = 7.31PDD41 pKa = 3.68PDD43 pKa = 3.92SQMEE47 pKa = 4.46TIDD50 pKa = 3.83RR51 pKa = 11.84LIKK54 pKa = 10.61FGFEE58 pKa = 3.66NCANNYY64 pKa = 7.59SHH66 pKa = 7.87FISRR70 pKa = 11.84MKK72 pKa = 10.4RR73 pKa = 11.84LKK75 pKa = 10.47KK76 pKa = 10.34LLRR79 pKa = 11.84KK80 pKa = 9.34EE81 pKa = 4.05FAEE84 pKa = 4.61GTDD87 pKa = 3.67LTSGQLFVDD96 pKa = 4.34LSTYY100 pKa = 10.42RR101 pKa = 11.84KK102 pKa = 9.48VFYY105 pKa = 10.27RR106 pKa = 11.84YY107 pKa = 10.14LDD109 pKa = 3.44LTLSEE114 pKa = 4.84RR115 pKa = 11.84SATEE119 pKa = 4.37CVMAWTQARR128 pKa = 11.84AAGLADD134 pKa = 3.09RR135 pKa = 11.84RR136 pKa = 11.84MIEE139 pKa = 3.8KK140 pKa = 10.38SLEE143 pKa = 4.08KK144 pKa = 10.74AEE146 pKa = 4.57KK147 pKa = 9.39TLSTPRR153 pKa = 11.84RR154 pKa = 11.84VYY156 pKa = 10.76LRR158 pKa = 11.84GRR160 pKa = 11.84WRR162 pKa = 11.84EE163 pKa = 3.58IHH165 pKa = 6.2AHH167 pKa = 4.56YY168 pKa = 10.5QGFIPVLEE176 pKa = 4.41PEE178 pKa = 4.63VPRR181 pKa = 11.84GPLLDD186 pKa = 3.5VVRR189 pKa = 11.84RR190 pKa = 11.84VANKK194 pKa = 9.25VRR196 pKa = 11.84GEE198 pKa = 4.07TAHH201 pKa = 7.01ISTGPHH207 pKa = 5.54ACLEE211 pKa = 4.16IKK213 pKa = 10.24QAFGGQAAGIAYY225 pKa = 9.67LCKK228 pKa = 9.88RR229 pKa = 11.84DD230 pKa = 3.93VLTVSYY236 pKa = 11.09DD237 pKa = 3.34PVTLEE242 pKa = 4.15RR243 pKa = 11.84TFHH246 pKa = 6.83PARR249 pKa = 11.84PCASAQDD256 pKa = 4.18LLSWAISRR264 pKa = 11.84ALDD267 pKa = 3.74APVHH271 pKa = 4.83TRR273 pKa = 11.84MVRR276 pKa = 11.84IVAVSEE282 pKa = 3.78PSKK285 pKa = 11.12ARR287 pKa = 11.84TVTVGALAYY296 pKa = 9.71QIILGVVSKK305 pKa = 10.67IFQPALASGISRR317 pKa = 11.84TGLEE321 pKa = 4.24GTRR324 pKa = 11.84NLYY327 pKa = 9.41EE328 pKa = 4.2ALNNDD333 pKa = 4.03FDD335 pKa = 4.78PSNGLWGPLRR345 pKa = 11.84DD346 pKa = 3.6MDD348 pKa = 3.85RR349 pKa = 11.84TGKK352 pKa = 10.4FPVFALTSDD361 pKa = 3.77LEE363 pKa = 4.18EE364 pKa = 4.16ATDD367 pKa = 3.84YY368 pKa = 11.88GDD370 pKa = 3.92LAVARR375 pKa = 11.84QILQALLMRR384 pKa = 11.84CRR386 pKa = 11.84DD387 pKa = 3.3IPGFPLGLAVLAKK400 pKa = 10.46SLFLSSRR407 pKa = 11.84IIIRR411 pKa = 11.84PGYY414 pKa = 9.77GHH416 pKa = 7.1GLVHH420 pKa = 7.4WFRR423 pKa = 11.84KK424 pKa = 8.87RR425 pKa = 11.84NGWLMGDD432 pKa = 3.53RR433 pKa = 11.84MTKK436 pKa = 9.96VVLTLAHH443 pKa = 6.89EE444 pKa = 4.3IGILSAGIQFARR456 pKa = 11.84ICGDD460 pKa = 3.55DD461 pKa = 3.6VFALSKK467 pKa = 11.05APAQLQRR474 pKa = 11.84YY475 pKa = 7.84HH476 pKa = 8.26KK477 pKa = 11.08VMTDD481 pKa = 3.22LGFKK485 pKa = 10.19ISEE488 pKa = 4.36DD489 pKa = 3.16DD490 pKa = 3.52HH491 pKa = 6.8FISRR495 pKa = 11.84RR496 pKa = 11.84ILFYY500 pKa = 10.92CEE502 pKa = 3.82EE503 pKa = 4.22VSLVPQDD510 pKa = 4.84AKK512 pKa = 11.16DD513 pKa = 3.76LPSVCNRR520 pKa = 11.84RR521 pKa = 11.84SEE523 pKa = 4.03PSCYY527 pKa = 9.88VDD529 pKa = 3.3YY530 pKa = 10.51PRR532 pKa = 11.84IRR534 pKa = 11.84LLLPIKK540 pKa = 10.82VEE542 pKa = 4.36TNALSYY548 pKa = 10.71TDD550 pKa = 3.04TGRR553 pKa = 11.84FHH555 pKa = 7.44LLGKK559 pKa = 8.41EE560 pKa = 3.97MRR562 pKa = 11.84WVFQNSSQQADD573 pKa = 3.62PFIRR577 pKa = 11.84ASLLQHH583 pKa = 6.71IAIAMPRR590 pKa = 11.84DD591 pKa = 3.44ILSPFLPQEE600 pKa = 3.98LAGDD604 pKa = 3.87GAFPHH609 pKa = 6.31SAEE612 pKa = 4.23FLLSVIEE619 pKa = 4.31RR620 pKa = 11.84KK621 pKa = 10.11SINYY625 pKa = 9.18DD626 pKa = 2.97EE627 pKa = 4.49CLYY630 pKa = 10.43RR631 pKa = 11.84IHH633 pKa = 7.09SLTHH637 pKa = 5.68GKK639 pKa = 8.71WGFRR643 pKa = 11.84YY644 pKa = 9.76LRR646 pKa = 11.84ADD648 pKa = 3.87NINEE652 pKa = 4.18VVHH655 pKa = 6.93KK656 pKa = 7.97YY657 pKa = 10.17HH658 pKa = 7.4QMVPKK663 pKa = 10.47LKK665 pKa = 10.31VLEE668 pKa = 4.46SVLPPDD674 pKa = 3.51AVVRR678 pKa = 11.84ASEE681 pKa = 4.1VLISSLKK688 pKa = 10.17VKK690 pKa = 10.49GLEE693 pKa = 4.25TPEE696 pKa = 3.76KK697 pKa = 10.09TFFRR701 pKa = 11.84LYY703 pKa = 10.43RR704 pKa = 11.84SYY706 pKa = 10.2YY707 pKa = 7.36WYY709 pKa = 10.65KK710 pKa = 10.26VLHH713 pKa = 5.91GMKK716 pKa = 10.43APVLNFDD723 pKa = 3.53EE724 pKa = 5.19DD725 pKa = 3.46RR726 pKa = 11.84TRR728 pKa = 11.84VHH730 pKa = 7.05GGRR733 pKa = 11.84EE734 pKa = 4.11FKK736 pKa = 10.92LDD738 pKa = 3.52IPPDD742 pKa = 3.54VLVEE746 pKa = 4.1RR747 pKa = 11.84FYY749 pKa = 9.29TTWRR753 pKa = 11.84DD754 pKa = 3.23SGFTFF759 pKa = 4.98

Molecular weight: 86.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

994

235

759

497.0

56.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.042 ± 0.736

1.408 ± 0.272

6.539 ± 1.376

5.231 ± 0.146

5.131 ± 0.61

5.835 ± 0.148

3.018 ± 0.019

5.131 ± 0.219

4.125 ± 0.973

10.865 ± 1.148

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.012 ± 0.056

2.213 ± 0.166

5.734 ± 0.938

2.817 ± 0.336

8.853 ± 0.248

6.338 ± 0.393

5.131 ± 0.195

7.042 ± 0.301

1.408 ± 0.272

4.125 ± 0.478
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski