Paenibacillus lutimineralis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5477 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S9UV79|A0A3S9UV79_9BACL ABC transporter ATP-binding protein OS=Paenibacillus lutimineralis OX=2707005 GN=EI981_07140 PE=4 SV=1
MM1 pKa = 7.34QDD3 pKa = 3.03EE4 pKa = 5.21LYY6 pKa = 11.19GIIADD11 pKa = 3.32ICYY14 pKa = 9.92FEE16 pKa = 5.81KK17 pKa = 11.18DD18 pKa = 3.52EE19 pKa = 4.07MHH21 pKa = 7.0PALSVVDD28 pKa = 4.75DD29 pKa = 4.21LAISSVMIVEE39 pKa = 4.67IIAMIEE45 pKa = 3.49ARR47 pKa = 11.84YY48 pKa = 9.67GFNIEE53 pKa = 4.05EE54 pKa = 4.51QIDD57 pKa = 3.9EE58 pKa = 4.44LLGCEE63 pKa = 4.64LLGDD67 pKa = 3.92MTTLITRR74 pKa = 11.84LGEE77 pKa = 4.36DD78 pKa = 3.74YY79 pKa = 11.27AVAQAALGRR88 pKa = 4.07

Molecular weight: 9.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q9IGZ5|A0A3Q9IGZ5_9BACL Uncharacterized protein OS=Paenibacillus lutimineralis OX=2707005 GN=EI981_27445 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.78VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5477

0

5477

1810786

14

4085

330.6

36.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.602 ± 0.036

0.771 ± 0.009

5.245 ± 0.025

6.865 ± 0.037

4.131 ± 0.024

7.3 ± 0.029

2.063 ± 0.018

7.196 ± 0.036

5.497 ± 0.03

10.097 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.887 ± 0.017

4.084 ± 0.029

3.845 ± 0.019

3.958 ± 0.023

4.827 ± 0.029

6.54 ± 0.025

5.3 ± 0.025

6.868 ± 0.026

1.236 ± 0.014

3.687 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski