Nostoc sphaeroides CCNUC1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Nostocaceae; Nostoc; Nostoc sphaeroides

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10233 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8W931|A0A5P8W931_9NOSO 3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Nostoc sphaeroides CCNUC1 OX=2653204 GN=panB PE=3 SV=1
MM1 pKa = 7.99DD2 pKa = 3.72MALIKK7 pKa = 10.82GSLGNDD13 pKa = 3.06NLTGTPEE20 pKa = 4.22ADD22 pKa = 4.32SIFGDD27 pKa = 4.01LGDD30 pKa = 6.2DD31 pKa = 3.52ILDD34 pKa = 4.06GLGGDD39 pKa = 4.65DD40 pKa = 4.69KK41 pKa = 11.5FVFNNVIDD49 pKa = 3.91VLVFDD54 pKa = 5.55DD55 pKa = 6.33FSDD58 pKa = 4.21LYY60 pKa = 10.58IYY62 pKa = 9.87STDD65 pKa = 3.16GFDD68 pKa = 3.52IIKK71 pKa = 10.33NFFSGEE77 pKa = 4.23VISIDD82 pKa = 3.44PVNHH86 pKa = 5.59NRR88 pKa = 11.84KK89 pKa = 8.94HH90 pKa = 6.72VDD92 pKa = 2.75INVVISAAPAVVFPSEE108 pKa = 4.24KK109 pKa = 9.84TEE111 pKa = 4.31SIYY114 pKa = 10.52GTTGSDD120 pKa = 3.72TIQGTSADD128 pKa = 3.98EE129 pKa = 4.27IIYY132 pKa = 10.32GLEE135 pKa = 4.35GNDD138 pKa = 3.18VLYY141 pKa = 11.31GNDD144 pKa = 4.83GYY146 pKa = 11.76DD147 pKa = 3.23MLYY150 pKa = 10.67GGSGNDD156 pKa = 3.36SLYY159 pKa = 11.29GGANFGDD166 pKa = 4.22EE167 pKa = 4.7LYY169 pKa = 11.11GGLGNDD175 pKa = 3.65YY176 pKa = 11.09LNGGGANVLYY186 pKa = 10.73GEE188 pKa = 4.73EE189 pKa = 4.62GDD191 pKa = 5.79DD192 pKa = 4.19IFDD195 pKa = 5.2GIDD198 pKa = 4.07GIDD201 pKa = 4.48GIDD204 pKa = 4.3GIDD207 pKa = 3.54GVKK210 pKa = 10.12VHH212 pKa = 6.93IIEE215 pKa = 4.38YY216 pKa = 9.93LNQGTDD222 pKa = 3.31TVQASSSYY230 pKa = 11.09NLGDD234 pKa = 3.48NLEE237 pKa = 4.13NLVLTGSSDD246 pKa = 2.97IDD248 pKa = 4.29GIGNALDD255 pKa = 3.71NRR257 pKa = 11.84IEE259 pKa = 4.26GNAGHH264 pKa = 6.63NFLIGGDD271 pKa = 3.82GNDD274 pKa = 3.45YY275 pKa = 11.11LLGYY279 pKa = 9.98GDD281 pKa = 4.37YY282 pKa = 10.83DD283 pKa = 3.59ILVGEE288 pKa = 4.5AGKK291 pKa = 8.9DD292 pKa = 3.51TLVGGAGNDD301 pKa = 3.66FLNGGSGNDD310 pKa = 3.83FLNGGAGNNTLTGDD324 pKa = 3.53AGADD328 pKa = 2.94TFVFNFRR335 pKa = 11.84SEE337 pKa = 4.44GIDD340 pKa = 3.14IIKK343 pKa = 10.19DD344 pKa = 3.38FRR346 pKa = 11.84YY347 pKa = 9.77LQSDD351 pKa = 4.55KK352 pKa = 11.05IQISTIGFGATSTKK366 pKa = 9.93EE367 pKa = 3.42FSYY370 pKa = 11.11NRR372 pKa = 11.84NTGALSFQGTQFATLEE388 pKa = 4.17NKK390 pKa = 10.09PNFIPRR396 pKa = 11.84LDD398 pKa = 3.64IEE400 pKa = 4.89LVSEE404 pKa = 4.71DD405 pKa = 3.88LSSSISYY412 pKa = 10.41ILL414 pKa = 3.82

Molecular weight: 43.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8W2P0|A0A5P8W2P0_9NOSO DNA-processing protein DprA OS=Nostoc sphaeroides CCNUC1 OX=2653204 GN=GXM_04444 PE=3 SV=1
MM1 pKa = 7.66LFRR4 pKa = 11.84RR5 pKa = 11.84THH7 pKa = 5.36LLFRR11 pKa = 11.84RR12 pKa = 11.84THH14 pKa = 5.49LLFRR18 pKa = 11.84RR19 pKa = 11.84THH21 pKa = 5.49LLFRR25 pKa = 11.84RR26 pKa = 11.84THH28 pKa = 5.49LLFRR32 pKa = 11.84RR33 pKa = 11.84THH35 pKa = 5.49LLFRR39 pKa = 11.84RR40 pKa = 11.84THH42 pKa = 5.49LLFRR46 pKa = 11.84RR47 pKa = 11.84THH49 pKa = 5.49LLFRR53 pKa = 11.84RR54 pKa = 11.84THH56 pKa = 5.49LLFRR60 pKa = 11.84RR61 pKa = 11.84THH63 pKa = 5.49LLFRR67 pKa = 11.84RR68 pKa = 11.84THH70 pKa = 5.36LLFRR74 pKa = 11.84RR75 pKa = 11.84VHH77 pKa = 5.46NAPYY81 pKa = 10.16KK82 pKa = 7.48ITRR85 pKa = 11.84RR86 pKa = 11.84VEE88 pKa = 3.95TYY90 pKa = 8.97TGHH93 pKa = 6.0NTVQLSPKK101 pKa = 9.33TLVKK105 pKa = 9.75TRR107 pKa = 11.84NFASLQVV114 pKa = 3.39

Molecular weight: 14.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10233

0

10233

2503695

37

4984

244.7

27.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.813 ± 0.03

1.021 ± 0.01

4.882 ± 0.025

6.268 ± 0.03

3.997 ± 0.02

6.538 ± 0.038

1.773 ± 0.015

6.911 ± 0.024

5.175 ± 0.026

10.891 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.795 ± 0.013

4.682 ± 0.031

4.54 ± 0.024

5.372 ± 0.025

5.054 ± 0.029

6.691 ± 0.026

5.704 ± 0.024

6.438 ± 0.022

1.405 ± 0.011

3.047 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski