Tanticharoenia sakaeratensis NBRC 103193
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3154 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D6MKM2|A0A0D6MKM2_9PROT Molybdopterin oxidoreductase OS=Tanticharoenia sakaeratensis NBRC 103193 OX=1231623 GN=Tasa_015_010 PE=3 SV=1
MM1 pKa = 7.36 GMVKK5 pKa = 10.07 DD6 pKa = 3.98 RR7 pKa = 11.84 SMSIFSSLDD16 pKa = 3.33 TAVSGLNAQSQAFSNLSNNISNSQTVGYY44 pKa = 9.63 KK45 pKa = 9.41 ATDD48 pKa = 3.14 TAFEE52 pKa = 4.49 DD53 pKa = 4.73 FVTNSASGNSGTLNDD68 pKa = 4.44 GGGSYY73 pKa = 10.6 SDD75 pKa = 3.75 SVAANTIQRR84 pKa = 11.84 NDD86 pKa = 3.32 VQGTITSSTNALALAISGNGFFNVQEE112 pKa = 4.26 ASGSSASSSPTFSGQQYY129 pKa = 6.37 YY130 pKa = 8.66 TRR132 pKa = 11.84 NGDD135 pKa = 3.47 FSRR138 pKa = 11.84 DD139 pKa = 3.36 ANGYY143 pKa = 9.05 LVNTSGQYY151 pKa = 10.47 LEE153 pKa = 5.39 GYY155 pKa = 9.21 QVGTNGQMGTTLQPLQIQNVAFTPTEE181 pKa = 4.19 STTMTVSGTIGSSTTSGASTQTTATAYY208 pKa = 9.99 SAQGTPQSVGLNWQQSTTNPLDD230 pKa = 3.36 WTVSNSADD238 pKa = 3.72 PTNSTAVTFNSDD250 pKa = 2.72 GTLASVGSEE259 pKa = 4.07 STSGATGSFSFTGSPQDD276 pKa = 3.25 MTVNLGTIGQSSGIALSTTTTSTASDD302 pKa = 3.09 ATMTTDD308 pKa = 3.1 SVTSGTFQGLSMQSDD323 pKa = 4.47 GSVMATFNNGLSQLVGKK340 pKa = 10.43 IPLATFANPNGLAPADD356 pKa = 3.87 GQAYY360 pKa = 6.7 TATEE364 pKa = 3.84 ASGGPTEE371 pKa = 4.36 NAVNTGAAGSLEE383 pKa = 4.27 TGSTEE388 pKa = 4.98 SSTTDD393 pKa = 3.07 LTGDD397 pKa = 4.11 LTQLIVAQQAYY408 pKa = 8.69 GANSKK413 pKa = 10.01 VVSTADD419 pKa = 3.07 QLMQTTIAMIQQ430 pKa = 3.01
Molecular weight: 43.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.592
IPC_protein 3.605
Toseland 3.376
ProMoST 3.783
Dawson 3.605
Bjellqvist 3.77
Wikipedia 3.567
Rodwell 3.427
Grimsley 3.287
Solomon 3.592
Lehninger 3.554
Nozaki 3.732
DTASelect 3.986
Thurlkill 3.439
EMBOSS 3.579
Sillero 3.719
Patrickios 0.947
IPC_peptide 3.579
IPC2_peptide 3.694
IPC2.peptide.svr19 3.703
Protein with the highest isoelectric point:
>tr|A0A0D6MJU6|A0A0D6MJU6_9PROT Protoheme IX farnesyltransferase OS=Tanticharoenia sakaeratensis NBRC 103193 OX=1231623 GN=ctaB PE=3 SV=1
MM1 pKa = 7.58 FFRR4 pKa = 11.84 KK5 pKa = 9.67 KK6 pKa = 9.88 SAPPAPQPTVTPGNAKK22 pKa = 9.36 PAAGRR27 pKa = 11.84 HH28 pKa = 4.94 IGGLTTINVGLTALASIGPRR48 pKa = 11.84 RR49 pKa = 11.84 FRR51 pKa = 11.84 PHH53 pKa = 5.8 AQAALAMIAAAQALWTLIRR72 pKa = 11.84 KK73 pKa = 9.22 SSS75 pKa = 3.12
Molecular weight: 7.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.445
IPC2_protein 10.965
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.457
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.193
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.074
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3154
0
3154
1028729
40
2868
326.2
35.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.061 ± 0.062
0.97 ± 0.013
5.953 ± 0.03
4.692 ± 0.04
3.406 ± 0.022
8.715 ± 0.038
2.504 ± 0.017
4.894 ± 0.028
2.102 ± 0.028
10.238 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.516 ± 0.019
2.404 ± 0.033
5.693 ± 0.035
3.202 ± 0.025
7.661 ± 0.045
5.534 ± 0.036
5.694 ± 0.039
7.201 ± 0.036
1.418 ± 0.016
2.141 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here