Lactobacillus sharpeae JCM 1186 = DSM 20505

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lacticaseibacillus; Lacticaseibacillus sharpeae

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2271 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R1ZJ15|A0A0R1ZJ15_9LACO Rpir family transcriptional regulator OS=Lactobacillus sharpeae JCM 1186 = DSM 20505 OX=1291052 GN=FC18_GL002262 PE=4 SV=1
MM1 pKa = 6.86EE2 pKa = 4.69QKK4 pKa = 10.59EE5 pKa = 4.51LLNSDD10 pKa = 3.46QLRR13 pKa = 11.84FIGKK17 pKa = 9.66AYY19 pKa = 10.74SEE21 pKa = 4.52ASASEE26 pKa = 3.86NGSYY30 pKa = 9.95QEE32 pKa = 4.26AWDD35 pKa = 4.01EE36 pKa = 4.05FDD38 pKa = 3.19ATGAFAQLDD47 pKa = 3.79QLAQTPNRR55 pKa = 11.84TSLIVFSPYY64 pKa = 11.06GNLLYY69 pKa = 10.04WIGSIVPAGTEE80 pKa = 4.12VPAGLQHH87 pKa = 6.79YY88 pKa = 7.61DD89 pKa = 3.63LPAHH93 pKa = 5.6TVARR97 pKa = 11.84LAKK100 pKa = 9.52PMTMMMNAYY109 pKa = 8.35PVNIAIQQGATALDD123 pKa = 3.64AAGFPLPEE131 pKa = 4.69YY132 pKa = 9.98IGQTATPYY140 pKa = 10.08YY141 pKa = 9.69VEE143 pKa = 5.06RR144 pKa = 11.84YY145 pKa = 9.54NLDD148 pKa = 3.26GDD150 pKa = 4.19SVKK153 pKa = 10.43EE154 pKa = 3.95VEE156 pKa = 4.23YY157 pKa = 9.99TVYY160 pKa = 10.94VGDD163 pKa = 4.02DD164 pKa = 3.23RR165 pKa = 11.84DD166 pKa = 3.59YY167 pKa = 11.97GFDD170 pKa = 3.82DD171 pKa = 4.28VEE173 pKa = 4.05

Molecular weight: 19.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R1ZKU6|A0A0R1ZKU6_9LACO Uncharacterized protein OS=Lactobacillus sharpeae JCM 1186 = DSM 20505 OX=1291052 GN=FC18_GL001219 PE=4 SV=1
MM1 pKa = 5.72TTKK4 pKa = 9.85RR5 pKa = 11.84TFQPKK10 pKa = 8.33KK11 pKa = 7.5RR12 pKa = 11.84HH13 pKa = 5.56RR14 pKa = 11.84EE15 pKa = 3.79RR16 pKa = 11.84VHH18 pKa = 7.7GFMKK22 pKa = 10.56RR23 pKa = 11.84MSTKK27 pKa = 9.52NGRR30 pKa = 11.84RR31 pKa = 11.84VLARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 9.97GRR41 pKa = 11.84KK42 pKa = 8.64VLAAA46 pKa = 4.31

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2271

0

2271

689515

46

2571

303.6

33.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.349 ± 0.072

0.432 ± 0.013

5.987 ± 0.058

4.883 ± 0.045

3.99 ± 0.04

7.098 ± 0.047

2.222 ± 0.023

6.24 ± 0.046

5.272 ± 0.052

9.708 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.778 ± 0.029

4.259 ± 0.035

3.784 ± 0.03

4.331 ± 0.034

4.607 ± 0.043

5.329 ± 0.044

6.833 ± 0.064

7.559 ± 0.04

1.038 ± 0.018

3.29 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski