Micromonas sp. RCC1109 virus MpV1

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Prasinovirus; unclassified Prasinovirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 244 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5EQD4|E5EQD4_9PHYC Uncharacterized protein OS=Micromonas sp. RCC1109 virus MpV1 OX=880161 GN=MpV1_097c PE=4 SV=1
MM1 pKa = 7.57NMQTGFGDD9 pKa = 4.04DD10 pKa = 3.54GSAMAEE16 pKa = 4.08QYY18 pKa = 8.56TKK20 pKa = 10.88TMIGILMPVMEE31 pKa = 5.23RR32 pKa = 11.84SMILAAEE39 pKa = 4.1YY40 pKa = 10.97SKK42 pKa = 11.59ACGRR46 pKa = 11.84DD47 pKa = 3.17TVLPEE52 pKa = 4.14DD53 pKa = 3.66MEE55 pKa = 4.61YY56 pKa = 11.46AMKK59 pKa = 10.47YY60 pKa = 9.04SAMYY64 pKa = 9.48TVGQNIGSLFPEE76 pKa = 5.12IYY78 pKa = 10.43DD79 pKa = 4.51EE80 pKa = 4.75EE81 pKa = 4.63QDD83 pKa = 4.05DD84 pKa = 4.54DD85 pKa = 5.69DD86 pKa = 7.23DD87 pKa = 5.25IEE89 pKa = 4.41EE90 pKa = 4.56VSPEE94 pKa = 3.89DD95 pKa = 3.49CPPFEE100 pKa = 5.0RR101 pKa = 11.84YY102 pKa = 9.42SGNDD106 pKa = 2.84EE107 pKa = 4.55RR108 pKa = 11.84FILMNHH114 pKa = 7.44AYY116 pKa = 10.3DD117 pKa = 3.4NWEE120 pKa = 3.48NWTPQNPTEE129 pKa = 4.19QMLKK133 pKa = 10.46NAINSNEE140 pKa = 3.89HH141 pKa = 5.67LL142 pKa = 4.11

Molecular weight: 16.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5EQF5|E5EQF5_9PHYC Uncharacterized protein OS=Micromonas sp. RCC1109 virus MpV1 OX=880161 GN=MpV1_118 PE=4 SV=1
MM1 pKa = 7.14VNRR4 pKa = 11.84MNINKK9 pKa = 9.15PPAEE13 pKa = 4.05KK14 pKa = 10.22RR15 pKa = 11.84KK16 pKa = 10.31SSNNLTTMFNTNKK29 pKa = 10.42KK30 pKa = 9.43ILKK33 pKa = 8.05QRR35 pKa = 11.84KK36 pKa = 8.83LIRR39 pKa = 11.84NLPPLPAGLGMSCTRR54 pKa = 11.84PGYY57 pKa = 9.87IRR59 pKa = 11.84YY60 pKa = 9.65LNEE63 pKa = 3.26IQKK66 pKa = 9.65YY67 pKa = 8.84FEE69 pKa = 3.6NRR71 pKa = 11.84RR72 pKa = 11.84YY73 pKa = 9.12NGRR76 pKa = 11.84RR77 pKa = 11.84IHH79 pKa = 5.5VRR81 pKa = 11.84FMEE84 pKa = 4.01YY85 pKa = 10.3HH86 pKa = 7.42DD87 pKa = 5.51DD88 pKa = 3.48IKK90 pKa = 10.88MGAIANKK97 pKa = 9.77SSQIINGNPSITFSNNSTPFPQINANPVGIYY128 pKa = 9.95YY129 pKa = 9.22FIITVQKK136 pKa = 10.68QEE138 pKa = 4.02GEE140 pKa = 4.01MAHH143 pKa = 7.19AINVLVDD150 pKa = 3.81PNRR153 pKa = 11.84LNPRR157 pKa = 11.84IWAFDD162 pKa = 3.36PHH164 pKa = 6.69GASSMNSNGFGSLLRR179 pKa = 11.84SKK181 pKa = 10.38IIPSIKK187 pKa = 10.2KK188 pKa = 9.6LFGNILVNRR197 pKa = 11.84TNVRR201 pKa = 11.84TMIYY205 pKa = 10.21NGPNLQARR213 pKa = 11.84NVRR216 pKa = 11.84GVCTTFHH223 pKa = 6.52ISFSEE228 pKa = 4.12VLLDD232 pKa = 4.69LLNEE236 pKa = 4.87KK237 pKa = 8.71ITISDD242 pKa = 4.28LGRR245 pKa = 11.84IIPNNPNFRR254 pKa = 11.84SWFLNRR260 pKa = 11.84PGPLQNVINKK270 pKa = 8.38EE271 pKa = 4.09VTKK274 pKa = 10.76VNLPISPMKK283 pKa = 8.88MTMGKK288 pKa = 9.62SNKK291 pKa = 9.0LKK293 pKa = 10.4KK294 pKa = 9.83VLRR297 pKa = 11.84RR298 pKa = 11.84RR299 pKa = 3.56

Molecular weight: 34.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

244

0

244

57882

65

1813

237.2

26.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.414 ± 0.188

1.731 ± 0.132

6.119 ± 0.136

6.646 ± 0.202

4.608 ± 0.143

6.254 ± 0.363

2.168 ± 0.111

6.876 ± 0.15

7.73 ± 0.359

7.816 ± 0.147

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.954 ± 0.102

5.883 ± 0.298

4.069 ± 0.135

3.098 ± 0.118

4.746 ± 0.166

6.156 ± 0.161

6.166 ± 0.212

6.589 ± 0.166

1.04 ± 0.061

3.937 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski