Streptococcus satellite phage Javan632
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZTL6|A0A4D5ZTL6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan632 OX=2558796 GN=JavanS632_0012 PE=4 SV=1
MM1 pKa = 7.77 KK2 pKa = 10.48 YY3 pKa = 9.79 SQQVLDD9 pKa = 3.86 MLEE12 pKa = 4.06 QAVSGQIDD20 pKa = 3.83 NFGDD24 pKa = 3.8 LYY26 pKa = 11.49 FEE28 pKa = 4.7 FNALFGEE35 pKa = 4.61 EE36 pKa = 3.95 QQ37 pKa = 3.07
Molecular weight: 4.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.921
IPC2_protein 3.719
IPC_protein 3.452
Toseland 3.325
ProMoST 3.694
Dawson 3.478
Bjellqvist 3.668
Wikipedia 3.465
Rodwell 3.338
Grimsley 3.261
Solomon 3.363
Lehninger 3.325
Nozaki 3.668
DTASelect 3.719
Thurlkill 3.439
EMBOSS 3.465
Sillero 3.592
Patrickios 1.799
IPC_peptide 3.363
IPC2_peptide 3.541
IPC2.peptide.svr19 3.672
Protein with the highest isoelectric point:
>tr|A0A4D5ZSS6|A0A4D5ZSS6_9VIRU Putative transcription regulator OS=Streptococcus satellite phage Javan632 OX=2558796 GN=JavanS632_0017 PE=4 SV=1
MM1 pKa = 7.79 NITEE5 pKa = 4.21 YY6 pKa = 10.63 KK7 pKa = 9.64 KK8 pKa = 10.65 KK9 pKa = 10.61 NGTIVYY15 pKa = 9.59 RR16 pKa = 11.84 SSVYY20 pKa = 10.79 LGVDD24 pKa = 2.7 KK25 pKa = 10.61 LTGKK29 pKa = 10.25 KK30 pKa = 10.31 ARR32 pKa = 11.84 TTVTANTKK40 pKa = 10.28 KK41 pKa = 10.41 GVKK44 pKa = 9.28 IKK46 pKa = 10.73 ARR48 pKa = 11.84 EE49 pKa = 4.2 AINAFTANGYY59 pKa = 7.95 CVKK62 pKa = 9.97 EE63 pKa = 4.32 KK64 pKa = 9.55 PTITTYY70 pKa = 10.98 RR71 pKa = 11.84 EE72 pKa = 4.14 LVALWWEE79 pKa = 4.57 SYY81 pKa = 11.28 KK82 pKa = 10.03 NTIKK86 pKa = 10.78 PNSQQSMEE94 pKa = 4.61 GIVRR98 pKa = 11.84 LHH100 pKa = 6.54 ILPVFGDD107 pKa = 3.7 YY108 pKa = 11.03 KK109 pKa = 10.72 LDD111 pKa = 3.64 KK112 pKa = 9.61 LTTPIIQQQVNKK124 pKa = 9.5 WADD127 pKa = 3.19 KK128 pKa = 10.86 ANRR131 pKa = 11.84 GVKK134 pKa = 9.36 GAYY137 pKa = 9.85 ANYY140 pKa = 10.76 SFLNNINRR148 pKa = 11.84 RR149 pKa = 11.84 ILQYY153 pKa = 10.72 GVTMQVIQYY162 pKa = 9.64 NPARR166 pKa = 11.84 DD167 pKa = 3.6 VIIPRR172 pKa = 11.84 KK173 pKa = 7.62 QQNKK177 pKa = 5.06 EE178 pKa = 3.84 HH179 pKa = 6.35 KK180 pKa = 10.3 VKK182 pKa = 10.6 FFSNQEE188 pKa = 3.76 LKK190 pKa = 10.87 QFLDD194 pKa = 3.59 YY195 pKa = 11.26 LDD197 pKa = 5.03 NLDD200 pKa = 3.85 LSSYY204 pKa = 11.52 EE205 pKa = 3.95 NFFDD209 pKa = 3.74 YY210 pKa = 11.18 ALYY213 pKa = 8.57 KK214 pKa = 10.08 TLLASGCRR222 pKa = 11.84 IGEE225 pKa = 4.0 ALALEE230 pKa = 4.49 WSDD233 pKa = 3.79 IDD235 pKa = 5.07 LDD237 pKa = 3.94 NGTISISKK245 pKa = 8.18 TLNRR249 pKa = 11.84 YY250 pKa = 8.7 QEE252 pKa = 4.35 TNTPKK257 pKa = 10.61 SKK259 pKa = 10.87 AGLRR263 pKa = 11.84 DD264 pKa = 3.36 IEE266 pKa = 4.14 IDD268 pKa = 3.29 KK269 pKa = 10.02 ATVLLLKK276 pKa = 10.18 QYY278 pKa = 11.2 KK279 pKa = 9.64 NRR281 pKa = 11.84 QQVQSWQLGRR291 pKa = 11.84 SEE293 pKa = 5.95 DD294 pKa = 3.51 IVFTPFSTKK303 pKa = 9.73 YY304 pKa = 10.61 AYY306 pKa = 10.13 ACRR309 pKa = 11.84 LRR311 pKa = 11.84 KK312 pKa = 9.41 RR313 pKa = 11.84 LQKK316 pKa = 10.23 HH317 pKa = 5.26 FKK319 pKa = 8.91 AAKK322 pKa = 8.73 VPDD325 pKa = 3.5 ISFHH329 pKa = 5.61 GFRR332 pKa = 11.84 HH333 pKa = 4.4 THH335 pKa = 4.65 ATIMLYY341 pKa = 10.62 AGIEE345 pKa = 4.18 AKK347 pKa = 10.42 DD348 pKa = 3.35 LQYY351 pKa = 11.55 RR352 pKa = 11.84 LGHH355 pKa = 6.02 SNISMTLNTYY365 pKa = 8.7 VHH367 pKa = 6.14 ATKK370 pKa = 10.47 EE371 pKa = 4.25 GAKK374 pKa = 9.7 KK375 pKa = 10.24 AVSIFEE381 pKa = 4.0 AAISNLL387 pKa = 3.46
Molecular weight: 44.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.114
IPC2_protein 9.253
IPC_protein 9.136
Toseland 9.853
ProMoST 9.56
Dawson 10.087
Bjellqvist 9.75
Wikipedia 10.248
Rodwell 10.526
Grimsley 10.16
Solomon 10.101
Lehninger 10.058
Nozaki 9.853
DTASelect 9.736
Thurlkill 9.926
EMBOSS 10.262
Sillero 9.984
Patrickios 9.428
IPC_peptide 10.087
IPC2_peptide 8.273
IPC2.peptide.svr19 8.207
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19
0
19
2572
37
387
135.4
15.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.182 ± 0.514
0.622 ± 0.15
5.715 ± 0.494
8.398 ± 0.663
4.121 ± 0.373
4.938 ± 0.356
1.866 ± 0.184
6.649 ± 0.443
9.409 ± 0.524
10.187 ± 0.717
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.138 ± 0.233
5.482 ± 0.367
2.372 ± 0.23
4.782 ± 0.57
4.666 ± 0.515
6.649 ± 0.394
5.871 ± 0.406
4.938 ± 0.434
0.894 ± 0.212
4.121 ± 0.43
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here