Capybara microvirus Cap3_SP_581
Average proteome isoelectric point is 5.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVU9|A0A4V1FVU9_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_581 OX=2585478 PE=4 SV=1
MM1 pKa = 7.58 ALYY4 pKa = 10.48 ACYY7 pKa = 10.51 LDD9 pKa = 4.67 DD10 pKa = 5.19 NNRR13 pKa = 11.84 EE14 pKa = 4.14 CMTLIADD21 pKa = 4.28 CSTLEE26 pKa = 3.97 EE27 pKa = 4.5 CLSISLEE34 pKa = 4.15 DD35 pKa = 3.39 CDD37 pKa = 4.15 YY38 pKa = 11.51 FNAISGNKK46 pKa = 9.78 LKK48 pKa = 10.75 VDD50 pKa = 3.65 YY51 pKa = 11.06 YY52 pKa = 11.21 KK53 pKa = 11.13 LCFRR57 pKa = 11.84 LNRR60 pKa = 11.84 SEE62 pKa = 4.46 RR63 pKa = 11.84 YY64 pKa = 10.02 VIII67 pKa = 4.62
Molecular weight: 7.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.418
IPC2_protein 4.571
IPC_protein 4.393
Toseland 4.202
ProMoST 4.52
Dawson 4.355
Bjellqvist 4.507
Wikipedia 4.253
Rodwell 4.215
Grimsley 4.126
Solomon 4.342
Lehninger 4.291
Nozaki 4.469
DTASelect 4.647
Thurlkill 4.24
EMBOSS 4.266
Sillero 4.495
Patrickios 0.299
IPC_peptide 4.342
IPC2_peptide 4.482
IPC2.peptide.svr19 4.445
Protein with the highest isoelectric point:
>tr|A0A4P8W8U9|A0A4P8W8U9_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_581 OX=2585478 PE=4 SV=1
MM1 pKa = 7.5 VILDD5 pKa = 3.89 DD6 pKa = 3.8 VVAVVFVVIVSIAVALGFNCVDD28 pKa = 3.81 CFHH31 pKa = 6.79 TRR33 pKa = 11.84 TIKK36 pKa = 10.83 RR37 pKa = 11.84 PDD39 pKa = 3.46 NGSPMVVPCGNCMACRR55 pKa = 11.84 IARR58 pKa = 11.84 SRR60 pKa = 11.84 EE61 pKa = 3.32 WSLRR65 pKa = 11.84 LLMEE69 pKa = 5.05 LEE71 pKa = 3.91 SHH73 pKa = 6.8 DD74 pKa = 4.36 FKK76 pKa = 11.71 GCFITLTYY84 pKa = 10.81 DD85 pKa = 4.27 DD86 pKa = 4.34 EE87 pKa = 5.04 HH88 pKa = 8.0 LPSDD92 pKa = 3.98 YY93 pKa = 11.19 GLHH96 pKa = 7.34 KK97 pKa = 10.73 DD98 pKa = 4.16 DD99 pKa = 6.16 LKK101 pKa = 11.56 NFWKK105 pKa = 10.63 RR106 pKa = 11.84 LRR108 pKa = 11.84 KK109 pKa = 10.1 DD110 pKa = 2.96 LDD112 pKa = 3.75 FKK114 pKa = 10.87 IKK116 pKa = 10.58 YY117 pKa = 6.86 YY118 pKa = 11.17 ACGEE122 pKa = 4.11 YY123 pKa = 10.53 GDD125 pKa = 4.28 TYY127 pKa = 11.23 GRR129 pKa = 11.84 PHH131 pKa = 5.37 YY132 pKa = 10.04 HH133 pKa = 6.99 AIIFGLEE140 pKa = 3.65 PDD142 pKa = 3.81 DD143 pKa = 3.52 FTRR146 pKa = 11.84 RR147 pKa = 11.84 LLIDD151 pKa = 2.98 NWRR154 pKa = 11.84 FCSPDD159 pKa = 2.99 RR160 pKa = 11.84 FNGTKK165 pKa = 9.88 QGLADD170 pKa = 3.69 VTIDD174 pKa = 3.44 SINYY178 pKa = 4.03 VTGYY182 pKa = 9.15 IQKK185 pKa = 10.33 KK186 pKa = 9.43 YY187 pKa = 10.91 NGDD190 pKa = 3.69 LAKK193 pKa = 10.25 EE194 pKa = 4.53 VYY196 pKa = 10.66 GDD198 pKa = 3.81 LQPPFSSSSQGLGLEE213 pKa = 4.24 TFLKK217 pKa = 9.62 NRR219 pKa = 11.84 EE220 pKa = 4.05 RR221 pKa = 11.84 FEE223 pKa = 5.32 HH224 pKa = 7.13 DD225 pKa = 3.22 GFIGYY230 pKa = 9.47 QGVQYY235 pKa = 8.56 TIPRR239 pKa = 11.84 YY240 pKa = 9.2 FIKK243 pKa = 10.69 KK244 pKa = 9.99 LDD246 pKa = 3.85 LEE248 pKa = 4.33 LSKK251 pKa = 9.85 TYY253 pKa = 9.14 STQKK257 pKa = 9.49 INEE260 pKa = 4.09 YY261 pKa = 10.38 LEE263 pKa = 4.5 KK264 pKa = 11.1 NGIDD268 pKa = 3.23 PSRR271 pKa = 11.84 FVKK274 pKa = 9.61 ATSPQFIKK282 pKa = 10.64 NVAYY286 pKa = 10.61 HH287 pKa = 6.31 EE288 pKa = 4.41 VADD291 pKa = 3.8 PHH293 pKa = 6.67 LAIAEE298 pKa = 4.05 RR299 pKa = 11.84 RR300 pKa = 11.84 LLRR303 pKa = 11.84 QKK305 pKa = 10.64 QLFEE309 pKa = 4.0 QDD311 pKa = 2.99 KK312 pKa = 11.06 LKK314 pKa = 11.17
Molecular weight: 36.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.672
IPC2_protein 6.825
IPC_protein 6.883
Toseland 6.751
ProMoST 7.512
Dawson 7.483
Bjellqvist 7.585
Wikipedia 7.424
Rodwell 7.483
Grimsley 6.839
Solomon 7.541
Lehninger 7.556
Nozaki 7.863
DTASelect 7.688
Thurlkill 7.717
EMBOSS 7.761
Sillero 7.951
Patrickios 4.101
IPC_peptide 7.541
IPC2_peptide 7.176
IPC2.peptide.svr19 7.121
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1418
67
559
236.3
26.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.206 ± 1.375
1.763 ± 0.725
7.123 ± 1.194
6.135 ± 0.718
5.078 ± 0.798
6.135 ± 1.008
2.116 ± 0.289
6.276 ± 0.742
5.783 ± 0.773
8.392 ± 0.585
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.468 ± 0.501
6.841 ± 0.931
3.879 ± 0.78
3.738 ± 0.707
4.725 ± 0.86
7.123 ± 0.888
4.795 ± 0.364
5.571 ± 0.338
1.058 ± 0.347
4.795 ± 0.385
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here