Escherichia virus Lambda_4A7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lambdavirus; unclassified Lambdavirus

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A653FRZ2|A0A653FRZ2_9CAUD Phage tail fiber protein OS=Escherichia virus Lambda_4A7 OX=2686055 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 9.4HH3 pKa = 5.21TEE5 pKa = 3.58LRR7 pKa = 11.84AAVLDD12 pKa = 3.88ALEE15 pKa = 4.33KK16 pKa = 10.45HH17 pKa = 6.2DD18 pKa = 4.25TGATLFDD25 pKa = 3.55GRR27 pKa = 11.84PAVFDD32 pKa = 4.2EE33 pKa = 4.31EE34 pKa = 5.13DD35 pKa = 3.96FPAVAVYY42 pKa = 8.14LTGAEE47 pKa = 4.22YY48 pKa = 9.85TGEE51 pKa = 4.09EE52 pKa = 4.61LDD54 pKa = 3.6SDD56 pKa = 3.79TWQAEE61 pKa = 3.69LHH63 pKa = 6.35IEE65 pKa = 4.19VFLPAQVPDD74 pKa = 4.05SEE76 pKa = 4.67LDD78 pKa = 2.98SWMEE82 pKa = 3.72SRR84 pKa = 11.84IYY86 pKa = 10.48PVMSDD91 pKa = 3.42VPALSDD97 pKa = 5.2LITSMVASGYY107 pKa = 10.36DD108 pKa = 3.32YY109 pKa = 11.21RR110 pKa = 11.84RR111 pKa = 11.84DD112 pKa = 3.5DD113 pKa = 5.7DD114 pKa = 4.64AGLWSSADD122 pKa = 3.34LTYY125 pKa = 11.13VITYY129 pKa = 9.4EE130 pKa = 3.98MM131 pKa = 4.92

Molecular weight: 14.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A653FQU0|A0A653FQU0_9CAUD Uncharacterized protein OS=Escherichia virus Lambda_4A7 OX=2686055 PE=4 SV=1
MM1 pKa = 6.44VTVAEE6 pKa = 4.25LQALRR11 pKa = 11.84QARR14 pKa = 11.84LDD16 pKa = 3.68LLTGKK21 pKa = 10.21RR22 pKa = 11.84VVSVQKK28 pKa = 10.06DD29 pKa = 3.54GRR31 pKa = 11.84RR32 pKa = 11.84IEE34 pKa = 4.02YY35 pKa = 8.47TAASLDD41 pKa = 3.71EE42 pKa = 4.7LNRR45 pKa = 11.84AINDD49 pKa = 3.65AEE51 pKa = 4.48SVLGTTRR58 pKa = 11.84CRR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84PLGVRR67 pKa = 11.84LL68 pKa = 3.64

Molecular weight: 7.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

14834

29

1132

211.9

23.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.627 ± 0.561

1.254 ± 0.174

5.568 ± 0.195

6.114 ± 0.32

3.694 ± 0.222

7.314 ± 0.26

1.881 ± 0.141

5.447 ± 0.292

5.42 ± 0.294

8.507 ± 0.384

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.905 ± 0.174

3.937 ± 0.238

3.93 ± 0.21

4.24 ± 0.314

6.236 ± 0.328

6.364 ± 0.227

5.953 ± 0.297

6.97 ± 0.271

1.611 ± 0.116

3.027 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski