Amino acid dipepetide frequency for Acidisphaera rubrifaciens HS-AP3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.54AlaAla: 23.54 ± 0.253	1.491AlaCys: 1.491 ± 0.048	7.937AlaAsp: 7.937 ± 0.096	7.512AlaGlu: 7.512 ± 0.125	4.389AlaPhe: 4.389 ± 0.077	13.283AlaGly: 13.283 ± 0.162	3.075AlaHis: 3.075 ± 0.054	6.1AlaIle: 6.1 ± 0.088	2.599AlaLys: 2.599 ± 0.063	14.602AlaLeu: 14.602 ± 0.18	3.771AlaMet: 3.771 ± 0.062	2.507AlaAsn: 2.507 ± 0.06	7.211AlaPro: 7.211 ± 0.106	4.011AlaGln: 4.011 ± 0.072	11.5AlaArg: 11.5 ± 0.137	5.991AlaSer: 5.991 ± 0.088	7.519AlaThr: 7.519 ± 0.117	10.358AlaVal: 10.358 ± 0.137	2.021AlaTrp: 2.021 ± 0.058	2.96AlaTyr: 2.96 ± 0.065	0.0AlaXaa: 0.0 ± 0.0
Cys	1.263CysAla: 1.263 ± 0.044	0.117CysCys: 0.117 ± 0.012	0.585CysAsp: 0.585 ± 0.027	0.366CysGlu: 0.366 ± 0.023	0.324CysPhe: 0.324 ± 0.018	1.039CysGly: 1.039 ± 0.036	0.263CysHis: 0.263 ± 0.02	0.398CysIle: 0.398 ± 0.02	0.162CysLys: 0.162 ± 0.014	0.882CysLeu: 0.882 ± 0.031	0.182CysMet: 0.182 ± 0.015	0.232CysAsn: 0.232 ± 0.015	0.528CysPro: 0.528 ± 0.028	0.236CysGln: 0.236 ± 0.018	0.775CysArg: 0.775 ± 0.033	0.407CysSer: 0.407 ± 0.024	0.507CysThr: 0.507 ± 0.026	0.752CysVal: 0.752 ± 0.035	0.136CysTrp: 0.136 ± 0.014	0.235CysTyr: 0.235 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	8.595AspAla: 8.595 ± 0.12	0.529AspCys: 0.529 ± 0.022	3.193AspAsp: 3.193 ± 0.07	2.938AspGlu: 2.938 ± 0.067	1.903AspPhe: 1.903 ± 0.048	6.005AspGly: 6.005 ± 0.099	1.376AspHis: 1.376 ± 0.052	2.731AspIle: 2.731 ± 0.054	0.967AspLys: 0.967 ± 0.037	5.743AspLeu: 5.743 ± 0.084	1.389AspMet: 1.389 ± 0.041	1.068AspAsn: 1.068 ± 0.042	3.972AspPro: 3.972 ± 0.068	1.464AspGln: 1.464 ± 0.046	4.565AspArg: 4.565 ± 0.088	1.963AspSer: 1.963 ± 0.053	2.895AspThr: 2.895 ± 0.054	4.364AspVal: 4.364 ± 0.067	1.058AspTrp: 1.058 ± 0.046	1.522AspTyr: 1.522 ± 0.048	0.0AspXaa: 0.0 ± 0.0
Glu	7.36GluAla: 7.36 ± 0.124	0.3GluCys: 0.3 ± 0.02	2.346GluAsp: 2.346 ± 0.059	2.271GluGlu: 2.271 ± 0.077	1.235GluPhe: 1.235 ± 0.036	3.891GluGly: 3.891 ± 0.072	1.17GluHis: 1.17 ± 0.041	2.513GluIle: 2.513 ± 0.072	1.128GluLys: 1.128 ± 0.044	4.203GluLeu: 4.203 ± 0.076	1.268GluMet: 1.268 ± 0.04	0.95GluAsn: 0.95 ± 0.032	2.227GluPro: 2.227 ± 0.051	1.64GluGln: 1.64 ± 0.051	4.513GluArg: 4.513 ± 0.099	1.852GluSer: 1.852 ± 0.05	3.146GluThr: 3.146 ± 0.07	3.552GluVal: 3.552 ± 0.077	0.597GluTrp: 0.597 ± 0.031	0.943GluTyr: 0.943 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	4.524PheAla: 4.524 ± 0.071	0.423PheCys: 0.423 ± 0.022	2.453PheAsp: 2.453 ± 0.055	1.533PheGlu: 1.533 ± 0.049	1.177PhePhe: 1.177 ± 0.043	3.404PheGly: 3.404 ± 0.069	0.788PheHis: 0.788 ± 0.028	1.421PheIle: 1.421 ± 0.046	0.613PheLys: 0.613 ± 0.031	3.044PheLeu: 3.044 ± 0.073	0.712PheMet: 0.712 ± 0.026	0.975PheAsn: 0.975 ± 0.035	1.662PhePro: 1.662 ± 0.045	0.819PheGln: 0.819 ± 0.03	2.378PheArg: 2.378 ± 0.05	1.635PheSer: 1.635 ± 0.051	1.891PheThr: 1.891 ± 0.052	2.647PheVal: 2.647 ± 0.063	0.507PheTrp: 0.507 ± 0.028	0.887PheTyr: 0.887 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	11.496GlyAla: 11.496 ± 0.141	0.924GlyCys: 0.924 ± 0.035	4.573GlyAsp: 4.573 ± 0.082	3.98GlyGlu: 3.98 ± 0.065	3.553GlyPhe: 3.553 ± 0.058	8.636GlyGly: 8.636 ± 0.155	2.242GlyHis: 2.242 ± 0.059	4.492GlyIle: 4.492 ± 0.077	2.071GlyLys: 2.071 ± 0.059	9.32GlyLeu: 9.32 ± 0.119	2.632GlyMet: 2.632 ± 0.062	1.95GlyAsn: 1.95 ± 0.07	4.409GlyPro: 4.409 ± 0.08	2.735GlyGln: 2.735 ± 0.058	7.698GlyArg: 7.698 ± 0.108	4.321GlySer: 4.321 ± 0.089	5.532GlyThr: 5.532 ± 0.137	6.779GlyVal: 6.779 ± 0.091	1.601GlyTrp: 1.601 ± 0.047	2.441GlyTyr: 2.441 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	3.282HisAla: 3.282 ± 0.072	0.241HisCys: 0.241 ± 0.017	1.506HisAsp: 1.506 ± 0.043	1.013HisGlu: 1.013 ± 0.03	0.782HisPhe: 0.782 ± 0.027	2.446HisGly: 2.446 ± 0.06	0.662HisHis: 0.662 ± 0.037	0.904HisIle: 0.904 ± 0.029	0.37HisLys: 0.37 ± 0.025	2.241HisLeu: 2.241 ± 0.058	0.558HisMet: 0.558 ± 0.028	0.467HisAsn: 0.467 ± 0.024	1.673HisPro: 1.673 ± 0.052	0.535HisGln: 0.535 ± 0.023	1.621HisArg: 1.621 ± 0.05	0.801HisSer: 0.801 ± 0.033	1.044HisThr: 1.044 ± 0.034	2.005HisVal: 2.005 ± 0.046	0.401HisTrp: 0.401 ± 0.022	0.607HisTyr: 0.607 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	7.119IleAla: 7.119 ± 0.103	0.474IleCys: 0.474 ± 0.025	3.399IleAsp: 3.399 ± 0.056	3.008IleGlu: 3.008 ± 0.07	1.221IlePhe: 1.221 ± 0.04	5.003IleGly: 5.003 ± 0.081	0.878IleHis: 0.878 ± 0.032	1.701IleIle: 1.701 ± 0.047	0.924IleLys: 0.924 ± 0.043	3.614IleLeu: 3.614 ± 0.062	0.812IleMet: 0.812 ± 0.031	1.216IleAsn: 1.216 ± 0.039	2.184IlePro: 2.184 ± 0.061	0.958IleGln: 0.958 ± 0.036	2.93IleArg: 2.93 ± 0.059	2.047IleSer: 2.047 ± 0.051	2.699IleThr: 2.699 ± 0.081	3.836IleVal: 3.836 ± 0.066	0.532IleTrp: 0.532 ± 0.03	1.088IleTyr: 1.088 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	2.393LysAla: 2.393 ± 0.071	0.123LysCys: 0.123 ± 0.013	0.965LysAsp: 0.965 ± 0.035	0.791LysGlu: 0.791 ± 0.037	0.516LysPhe: 0.516 ± 0.025	1.616LysGly: 1.616 ± 0.048	0.383LysHis: 0.383 ± 0.023	0.987LysIle: 0.987 ± 0.038	0.621LysLys: 0.621 ± 0.036	2.175LysLeu: 2.175 ± 0.056	0.477LysMet: 0.477 ± 0.025	0.413LysAsn: 0.413 ± 0.023	1.439LysPro: 1.439 ± 0.048	0.698LysGln: 0.698 ± 0.03	1.586LysArg: 1.586 ± 0.045	1.026LysSer: 1.026 ± 0.038	1.318LysThr: 1.318 ± 0.046	1.525LysVal: 1.525 ± 0.055	0.234LysTrp: 0.234 ± 0.017	0.489LysTyr: 0.489 ± 0.028	0.0LysXaa: 0.0 ± 0.0
Leu	15.094LeuAla: 15.094 ± 0.154	0.975LeuCys: 0.975 ± 0.033	6.262LeuAsp: 6.262 ± 0.083	4.107LeuGlu: 4.107 ± 0.078	3.324LeuPhe: 3.324 ± 0.072	8.731LeuGly: 8.731 ± 0.117	2.379LeuHis: 2.379 ± 0.052	4.544LeuIle: 4.544 ± 0.076	1.96LeuLys: 1.96 ± 0.056	10.343LeuLeu: 10.343 ± 0.148	2.099LeuMet: 2.099 ± 0.056	2.027LeuAsn: 2.027 ± 0.053	6.329LeuPro: 6.329 ± 0.095	2.521LeuGln: 2.521 ± 0.058	8.678LeuArg: 8.678 ± 0.119	5.349LeuSer: 5.349 ± 0.079	5.398LeuThr: 5.398 ± 0.09	7.416LeuVal: 7.416 ± 0.11	1.147LeuTrp: 1.147 ± 0.038	2.105LeuTyr: 2.105 ± 0.04	0.001LeuXaa: 0.001 ± 0.001
Met	3.146MetAla: 3.146 ± 0.059	0.177MetCys: 0.177 ± 0.015	1.17MetAsp: 1.17 ± 0.037	0.924MetGlu: 0.924 ± 0.035	0.69MetPhe: 0.69 ± 0.034	1.721MetGly: 1.721 ± 0.049	0.534MetHis: 0.534 ± 0.029	1.137MetIle: 1.137 ± 0.043	0.615MetLys: 0.615 ± 0.03	2.582MetLeu: 2.582 ± 0.06	0.549MetMet: 0.549 ± 0.026	0.643MetAsn: 0.643 ± 0.024	1.698MetPro: 1.698 ± 0.053	0.879MetGln: 0.879 ± 0.034	2.115MetArg: 2.115 ± 0.051	1.437MetSer: 1.437 ± 0.044	1.835MetThr: 1.835 ± 0.043	1.567MetVal: 1.567 ± 0.045	0.212MetTrp: 0.212 ± 0.017	0.35MetTyr: 0.35 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.733AsnAla: 2.733 ± 0.067	0.216AsnCys: 0.216 ± 0.018	1.137AsnAsp: 1.137 ± 0.044	0.919AsnGlu: 0.919 ± 0.036	0.744AsnPhe: 0.744 ± 0.03	2.068AsnGly: 2.068 ± 0.062	0.443AsnHis: 0.443 ± 0.022	1.006AsnIle: 1.006 ± 0.034	0.451AsnLys: 0.451 ± 0.027	2.216AsnLeu: 2.216 ± 0.056	0.529AsnMet: 0.529 ± 0.028	0.586AsnAsn: 0.586 ± 0.038	1.664AsnPro: 1.664 ± 0.045	0.596AsnGln: 0.596 ± 0.031	1.546AsnArg: 1.546 ± 0.042	0.829AsnSer: 0.829 ± 0.039	1.221AsnThr: 1.221 ± 0.045	1.814AsnVal: 1.814 ± 0.057	0.357AsnTrp: 0.357 ± 0.023	0.653AsnTyr: 0.653 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	8.568ProAla: 8.568 ± 0.127	0.423ProCys: 0.423 ± 0.023	4.631ProAsp: 4.631 ± 0.081	3.095ProGlu: 3.095 ± 0.061	1.878ProPhe: 1.878 ± 0.048	5.72ProGly: 5.72 ± 0.094	1.352ProHis: 1.352 ± 0.04	2.175ProIle: 2.175 ± 0.05	1.054ProLys: 1.054 ± 0.04	4.979ProLeu: 4.979 ± 0.075	1.258ProMet: 1.258 ± 0.039	1.288ProAsn: 1.288 ± 0.044	3.931ProPro: 3.931 ± 0.093	1.525ProGln: 1.525 ± 0.043	3.807ProArg: 3.807 ± 0.068	2.75ProSer: 2.75 ± 0.057	3.025ProThr: 3.025 ± 0.06	4.771ProVal: 4.771 ± 0.076	0.838ProTrp: 0.838 ± 0.034	1.397ProTyr: 1.397 ± 0.046	0.0ProXaa: 0.0 ± 0.0
Gln	4.163GlnAla: 4.163 ± 0.078	0.183GlnCys: 0.183 ± 0.015	1.371GlnAsp: 1.371 ± 0.038	1.121GlnGlu: 1.121 ± 0.039	0.913GlnPhe: 0.913 ± 0.032	2.221GlnGly: 2.221 ± 0.048	0.648GlnHis: 0.648 ± 0.028	1.439GlnIle: 1.439 ± 0.047	0.656GlnLys: 0.656 ± 0.03	2.467GlnLeu: 2.467 ± 0.057	0.762GlnMet: 0.762 ± 0.028	0.661GlnAsn: 0.661 ± 0.031	1.722GlnPro: 1.722 ± 0.051	1.036GlnGln: 1.036 ± 0.042	2.245GlnArg: 2.245 ± 0.056	1.466GlnSer: 1.466 ± 0.044	1.736GlnThr: 1.736 ± 0.05	2.302GlnVal: 2.302 ± 0.055	0.339GlnTrp: 0.339 ± 0.02	0.622GlnTyr: 0.622 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	11.045ArgAla: 11.045 ± 0.143	0.669ArgCys: 0.669 ± 0.029	4.725ArgAsp: 4.725 ± 0.082	3.668ArgGlu: 3.668 ± 0.073	3.0ArgPhe: 3.0 ± 0.06	5.774ArgGly: 5.774 ± 0.105	2.237ArgHis: 2.237 ± 0.054	3.964ArgIle: 3.964 ± 0.07	1.386ArgLys: 1.386 ± 0.046	9.133ArgLeu: 9.133 ± 0.121	2.014ArgMet: 2.014 ± 0.049	1.627ArgAsn: 1.627 ± 0.038	4.608ArgPro: 4.608 ± 0.082	2.325ArgGln: 2.325 ± 0.055	7.621ArgArg: 7.621 ± 0.118	3.186ArgSer: 3.186 ± 0.067	3.851ArgThr: 3.851 ± 0.072	5.955ArgVal: 5.955 ± 0.086	1.148ArgTrp: 1.148 ± 0.041	1.858ArgTyr: 1.858 ± 0.057	0.0ArgXaa: 0.0 ± 0.0
Ser	5.561SerAla: 5.561 ± 0.082	0.413SerCys: 0.413 ± 0.023	2.545SerAsp: 2.545 ± 0.056	1.892SerGlu: 1.892 ± 0.051	1.849SerPhe: 1.849 ± 0.042	4.924SerGly: 4.924 ± 0.105	0.946SerHis: 0.946 ± 0.038	2.157SerIle: 2.157 ± 0.053	0.878SerLys: 0.878 ± 0.033	4.735SerLeu: 4.735 ± 0.085	1.078SerMet: 1.078 ± 0.035	1.094SerAsn: 1.094 ± 0.044	2.635SerPro: 2.635 ± 0.058	1.143SerGln: 1.143 ± 0.034	3.121SerArg: 3.121 ± 0.073	2.135SerSer: 2.135 ± 0.062	2.29SerThr: 2.29 ± 0.066	3.838SerVal: 3.838 ± 0.072	0.588SerTrp: 0.588 ± 0.026	1.161SerTyr: 1.161 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	7.083ThrAla: 7.083 ± 0.121	0.477ThrCys: 0.477 ± 0.024	3.066ThrAsp: 3.066 ± 0.069	2.544ThrGlu: 2.544 ± 0.053	1.877ThrPhe: 1.877 ± 0.05	5.551ThrGly: 5.551 ± 0.11	1.234ThrHis: 1.234 ± 0.042	2.634ThrIle: 2.634 ± 0.07	0.991ThrLys: 0.991 ± 0.031	6.403ThrLeu: 6.403 ± 0.105	1.23ThrMet: 1.23 ± 0.037	1.218ThrAsn: 1.218 ± 0.04	3.82ThrPro: 3.82 ± 0.078	1.578ThrGln: 1.578 ± 0.054	3.753ThrArg: 3.753 ± 0.067	2.425ThrSer: 2.425 ± 0.061	3.268ThrThr: 3.268 ± 0.091	4.856ThrVal: 4.856 ± 0.102	0.83ThrTrp: 0.83 ± 0.032	1.424ThrTyr: 1.424 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	10.858ValAla: 10.858 ± 0.117	0.818ValCys: 0.818 ± 0.028	4.063ValAsp: 4.063 ± 0.081	3.788ValGlu: 3.788 ± 0.075	2.756ValPhe: 2.756 ± 0.065	5.921ValGly: 5.921 ± 0.085	1.586ValHis: 1.586 ± 0.051	3.943ValIle: 3.943 ± 0.065	1.553ValLys: 1.553 ± 0.046	8.249ValLeu: 8.249 ± 0.115	1.873ValMet: 1.873 ± 0.044	1.815ValAsn: 1.815 ± 0.048	4.713ValPro: 4.713 ± 0.074	2.123ValGln: 2.123 ± 0.054	5.988ValArg: 5.988 ± 0.08	3.545ValSer: 3.545 ± 0.071	4.951ValThr: 4.951 ± 0.091	6.608ValVal: 6.608 ± 0.112	1.063ValTrp: 1.063 ± 0.035	1.559ValTyr: 1.559 ± 0.047	0.0ValXaa: 0.0 ± 0.0
Trp	1.42TrpAla: 1.42 ± 0.044	0.151TrpCys: 0.151 ± 0.013	0.782TrpAsp: 0.782 ± 0.033	0.574TrpGlu: 0.574 ± 0.024	0.508TrpPhe: 0.508 ± 0.027	1.051TrpGly: 1.051 ± 0.037	0.448TrpHis: 0.448 ± 0.023	0.634TrpIle: 0.634 ± 0.03	0.315TrpLys: 0.315 ± 0.02	1.598TrpLeu: 1.598 ± 0.049	0.35TrpMet: 0.35 ± 0.018	0.407TrpAsn: 0.407 ± 0.023	0.778TrpPro: 0.778 ± 0.03	0.566TrpGln: 0.566 ± 0.026	1.466TrpArg: 1.466 ± 0.041	0.792TrpSer: 0.792 ± 0.034	0.914TrpThr: 0.914 ± 0.033	0.858TrpVal: 0.858 ± 0.031	0.262TrpTrp: 0.262 ± 0.017	0.362TrpTyr: 0.362 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.134TyrAla: 3.134 ± 0.063	0.264TyrCys: 0.264 ± 0.019	1.531TyrAsp: 1.531 ± 0.049	1.06TyrGlu: 1.06 ± 0.033	0.855TyrPhe: 0.855 ± 0.032	2.38TyrGly: 2.38 ± 0.058	0.513TyrHis: 0.513 ± 0.025	0.762TyrIle: 0.762 ± 0.029	0.464TyrLys: 0.464 ± 0.026	2.26TyrLeu: 2.26 ± 0.053	0.439TyrMet: 0.439 ± 0.021	0.582TyrAsn: 0.582 ± 0.029	1.261TyrPro: 1.261 ± 0.041	0.685TyrGln: 0.685 ± 0.03	1.891TyrArg: 1.891 ± 0.049	1.029TyrSer: 1.029 ± 0.036	1.22TyrThr: 1.22 ± 0.037	1.97TyrVal: 1.97 ± 0.051	0.364TyrTrp: 0.364 ± 0.024	0.682TyrTyr: 0.682 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.004XaaXaa: 0.004 ± 0.003

Statistics based on 2788 proteins (851939 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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