Amino acid dipepetide frequency for Sphingomonas sp. SRS2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.363AlaAla: 19.363 ± 0.188	1.039AlaCys: 1.039 ± 0.032	7.755AlaAsp: 7.755 ± 0.089	7.801AlaGlu: 7.801 ± 0.101	4.357AlaPhe: 4.357 ± 0.059	11.33AlaGly: 11.33 ± 0.091	2.292AlaHis: 2.292 ± 0.042	7.399AlaIle: 7.399 ± 0.092	4.138AlaLys: 4.138 ± 0.07	13.536AlaLeu: 13.536 ± 0.138	4.024AlaMet: 4.024 ± 0.058	2.993AlaAsn: 2.993 ± 0.051	5.953AlaPro: 5.953 ± 0.084	4.212AlaGln: 4.212 ± 0.06	9.847AlaArg: 9.847 ± 0.111	6.589AlaSer: 6.589 ± 0.077	6.485AlaThr: 6.485 ± 0.074	8.494AlaVal: 8.494 ± 0.082	1.598AlaTrp: 1.598 ± 0.04	2.681AlaTyr: 2.681 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	0.973CysAla: 0.973 ± 0.032	0.087CysCys: 0.087 ± 0.008	0.541CysAsp: 0.541 ± 0.02	0.385CysGlu: 0.385 ± 0.018	0.308CysPhe: 0.308 ± 0.018	0.848CysGly: 0.848 ± 0.026	0.237CysHis: 0.237 ± 0.02	0.38CysIle: 0.38 ± 0.016	0.165CysLys: 0.165 ± 0.012	0.708CysLeu: 0.708 ± 0.022	0.143CysMet: 0.143 ± 0.013	0.176CysAsn: 0.176 ± 0.012	0.442CysPro: 0.442 ± 0.022	0.19CysGln: 0.19 ± 0.013	0.583CysArg: 0.583 ± 0.023	0.436CysSer: 0.436 ± 0.022	0.368CysThr: 0.368 ± 0.017	0.516CysVal: 0.516 ± 0.02	0.114CysTrp: 0.114 ± 0.01	0.178CysTyr: 0.178 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.75AspAla: 7.75 ± 0.085	0.472AspCys: 0.472 ± 0.021	3.609AspAsp: 3.609 ± 0.059	3.382AspGlu: 3.382 ± 0.051	2.134AspPhe: 2.134 ± 0.043	5.59AspGly: 5.59 ± 0.069	1.438AspHis: 1.438 ± 0.036	3.408AspIle: 3.408 ± 0.049	1.78AspLys: 1.78 ± 0.037	5.897AspLeu: 5.897 ± 0.075	1.411AspMet: 1.411 ± 0.031	1.36AspAsn: 1.36 ± 0.039	3.822AspPro: 3.822 ± 0.056	1.91AspGln: 1.91 ± 0.046	5.215AspArg: 5.215 ± 0.08	2.515AspSer: 2.515 ± 0.049	2.422AspThr: 2.422 ± 0.047	4.083AspVal: 4.083 ± 0.059	1.067AspTrp: 1.067 ± 0.031	1.649AspTyr: 1.649 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	7.765GluAla: 7.765 ± 0.103	0.308GluCys: 0.308 ± 0.015	2.761GluAsp: 2.761 ± 0.052	2.8GluGlu: 2.8 ± 0.055	1.43GluPhe: 1.43 ± 0.037	4.353GluGly: 4.353 ± 0.063	1.209GluHis: 1.209 ± 0.029	3.191GluIle: 3.191 ± 0.047	1.972GluLys: 1.972 ± 0.041	5.195GluLeu: 5.195 ± 0.065	1.416GluMet: 1.416 ± 0.039	1.328GluAsn: 1.328 ± 0.031	2.583GluPro: 2.583 ± 0.048	2.095GluGln: 2.095 ± 0.045	5.03GluArg: 5.03 ± 0.076	2.311GluSer: 2.311 ± 0.044	3.07GluThr: 3.07 ± 0.055	3.443GluVal: 3.443 ± 0.06	0.71GluTrp: 0.71 ± 0.024	0.906GluTyr: 0.906 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	4.663PheAla: 4.663 ± 0.064	0.306PheCys: 0.306 ± 0.015	2.76PheAsp: 2.76 ± 0.053	2.046PheGlu: 2.046 ± 0.034	1.258PhePhe: 1.258 ± 0.034	3.606PheGly: 3.606 ± 0.057	0.754PheHis: 0.754 ± 0.025	1.543PheIle: 1.543 ± 0.035	0.894PheLys: 0.894 ± 0.031	3.125PheLeu: 3.125 ± 0.058	0.709PheMet: 0.709 ± 0.022	0.966PheAsn: 0.966 ± 0.029	1.51PhePro: 1.51 ± 0.038	0.93PheGln: 0.93 ± 0.024	2.316PheArg: 2.316 ± 0.046	2.012PheSer: 2.012 ± 0.039	1.97PheThr: 1.97 ± 0.047	2.464PheVal: 2.464 ± 0.046	0.489PheTrp: 0.489 ± 0.021	0.868PheTyr: 0.868 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	9.71GlyAla: 9.71 ± 0.095	0.785GlyCys: 0.785 ± 0.024	5.104GlyAsp: 5.104 ± 0.064	4.799GlyGlu: 4.799 ± 0.067	3.669GlyPhe: 3.669 ± 0.055	8.1GlyGly: 8.1 ± 0.124	1.888GlyHis: 1.888 ± 0.033	4.531GlyIle: 4.531 ± 0.06	3.36GlyLys: 3.36 ± 0.069	8.712GlyLeu: 8.712 ± 0.094	2.269GlyMet: 2.269 ± 0.039	2.241GlyAsn: 2.241 ± 0.056	3.625GlyPro: 3.625 ± 0.059	2.979GlyGln: 2.979 ± 0.052	6.722GlyArg: 6.722 ± 0.079	4.867GlySer: 4.867 ± 0.075	4.443GlyThr: 4.443 ± 0.078	6.176GlyVal: 6.176 ± 0.074	1.629GlyTrp: 1.629 ± 0.04	2.325GlyTyr: 2.325 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.42HisAla: 2.42 ± 0.052	0.224HisCys: 0.224 ± 0.013	1.32HisAsp: 1.32 ± 0.038	0.973HisGlu: 0.973 ± 0.028	0.853HisPhe: 0.853 ± 0.027	1.926HisGly: 1.926 ± 0.047	0.615HisHis: 0.615 ± 0.027	1.028HisIle: 1.028 ± 0.032	0.5HisLys: 0.5 ± 0.018	1.919HisLeu: 1.919 ± 0.044	0.494HisMet: 0.494 ± 0.017	0.479HisAsn: 0.479 ± 0.019	1.305HisPro: 1.305 ± 0.032	0.582HisGln: 0.582 ± 0.021	1.626HisArg: 1.626 ± 0.034	0.959HisSer: 0.959 ± 0.026	0.595HisThr: 0.595 ± 0.023	1.496HisVal: 1.496 ± 0.035	0.371HisTrp: 0.371 ± 0.017	0.561HisTyr: 0.561 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	8.407IleAla: 8.407 ± 0.086	0.46IleCys: 0.46 ± 0.02	4.411IleAsp: 4.411 ± 0.055	4.018IleGlu: 4.018 ± 0.065	1.805IlePhe: 1.805 ± 0.037	5.485IleGly: 5.485 ± 0.066	0.937IleHis: 0.937 ± 0.026	2.444IleIle: 2.444 ± 0.049	1.315IleLys: 1.315 ± 0.033	4.351IleLeu: 4.351 ± 0.07	0.93IleMet: 0.93 ± 0.027	1.383IleAsn: 1.383 ± 0.034	2.314IlePro: 2.314 ± 0.046	1.314IleGln: 1.314 ± 0.034	3.534IleArg: 3.534 ± 0.046	2.904IleSer: 2.904 ± 0.045	2.777IleThr: 2.777 ± 0.056	4.289IleVal: 4.289 ± 0.061	0.666IleTrp: 0.666 ± 0.025	1.116IleTyr: 1.116 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	4.291LysAla: 4.291 ± 0.069	0.141LysCys: 0.141 ± 0.011	1.514LysAsp: 1.514 ± 0.04	1.226LysGlu: 1.226 ± 0.036	0.8LysPhe: 0.8 ± 0.024	2.676LysGly: 2.676 ± 0.05	0.543LysHis: 0.543 ± 0.021	1.652LysIle: 1.652 ± 0.04	1.125LysLys: 1.125 ± 0.033	3.442LysLeu: 3.442 ± 0.057	0.806LysMet: 0.806 ± 0.029	0.762LysAsn: 0.762 ± 0.025	2.059LysPro: 2.059 ± 0.04	0.884LysGln: 0.884 ± 0.029	2.329LysArg: 2.329 ± 0.038	1.642LysSer: 1.642 ± 0.038	1.727LysThr: 1.727 ± 0.043	2.167LysVal: 2.167 ± 0.053	0.354LysTrp: 0.354 ± 0.017	0.558LysTyr: 0.558 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	13.566LeuAla: 13.566 ± 0.145	0.793LeuCys: 0.793 ± 0.026	6.459LeuAsp: 6.459 ± 0.082	4.578LeuGlu: 4.578 ± 0.063	3.683LeuPhe: 3.683 ± 0.068	8.332LeuGly: 8.332 ± 0.085	1.903LeuHis: 1.903 ± 0.041	5.508LeuIle: 5.508 ± 0.065	3.068LeuLys: 3.068 ± 0.054	9.814LeuLeu: 9.814 ± 0.108	2.164LeuMet: 2.164 ± 0.046	2.443LeuAsn: 2.443 ± 0.044	5.541LeuPro: 5.541 ± 0.07	2.47LeuGln: 2.47 ± 0.046	7.104LeuArg: 7.104 ± 0.082	6.243LeuSer: 6.243 ± 0.076	5.459LeuThr: 5.459 ± 0.07	6.729LeuVal: 6.729 ± 0.073	1.271LeuTrp: 1.271 ± 0.034	1.961LeuTyr: 1.961 ± 0.039	0.0LeuXaa: 0.0 ± 0.0
Met	3.324MetAla: 3.324 ± 0.051	0.146MetCys: 0.146 ± 0.01	1.035MetAsp: 1.035 ± 0.03	1.014MetGlu: 1.014 ± 0.028	0.673MetPhe: 0.673 ± 0.024	1.771MetGly: 1.771 ± 0.039	0.44MetHis: 0.44 ± 0.02	1.599MetIle: 1.599 ± 0.035	0.934MetLys: 0.934 ± 0.029	2.798MetLeu: 2.798 ± 0.056	0.7MetMet: 0.7 ± 0.024	0.666MetAsn: 0.666 ± 0.022	1.543MetPro: 1.543 ± 0.035	0.677MetGln: 0.677 ± 0.021	1.866MetArg: 1.866 ± 0.039	1.54MetSer: 1.54 ± 0.037	1.789MetThr: 1.789 ± 0.035	1.642MetVal: 1.642 ± 0.036	0.23MetTrp: 0.23 ± 0.013	0.254MetTyr: 0.254 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.199AsnAla: 3.199 ± 0.055	0.217AsnCys: 0.217 ± 0.015	1.368AsnAsp: 1.368 ± 0.035	1.109AsnGlu: 1.109 ± 0.028	0.909AsnPhe: 0.909 ± 0.027	2.384AsnGly: 2.384 ± 0.045	0.461AsnHis: 0.461 ± 0.02	1.41AsnIle: 1.41 ± 0.04	0.656AsnLys: 0.656 ± 0.025	2.463AsnLeu: 2.463 ± 0.049	0.596AsnMet: 0.596 ± 0.019	0.666AsnAsn: 0.666 ± 0.026	1.593AsnPro: 1.593 ± 0.038	0.704AsnGln: 0.704 ± 0.024	1.88AsnArg: 1.88 ± 0.037	1.289AsnSer: 1.289 ± 0.034	1.026AsnThr: 1.026 ± 0.035	1.797AsnVal: 1.797 ± 0.044	0.406AsnTrp: 0.406 ± 0.019	0.648AsnTyr: 0.648 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	6.936ProAla: 6.936 ± 0.089	0.323ProCys: 0.323 ± 0.017	3.694ProAsp: 3.694 ± 0.053	3.294ProGlu: 3.294 ± 0.054	1.865ProPhe: 1.865 ± 0.043	4.627ProGly: 4.627 ± 0.07	1.0ProHis: 1.0 ± 0.029	2.732ProIle: 2.732 ± 0.051	1.608ProLys: 1.608 ± 0.041	4.852ProLeu: 4.852 ± 0.064	1.206ProMet: 1.206 ± 0.03	1.253ProAsn: 1.253 ± 0.034	2.914ProPro: 2.914 ± 0.071	1.572ProGln: 1.572 ± 0.038	3.18ProArg: 3.18 ± 0.057	2.785ProSer: 2.785 ± 0.045	2.549ProThr: 2.549 ± 0.049	4.068ProVal: 4.068 ± 0.055	0.635ProTrp: 0.635 ± 0.024	1.127ProTyr: 1.127 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	4.047GlnAla: 4.047 ± 0.063	0.2GlnCys: 0.2 ± 0.013	1.381GlnAsp: 1.381 ± 0.032	1.269GlnGlu: 1.269 ± 0.037	1.011GlnPhe: 1.011 ± 0.027	2.503GlnGly: 2.503 ± 0.046	0.615GlnHis: 0.615 ± 0.022	1.808GlnIle: 1.808 ± 0.037	0.964GlnLys: 0.964 ± 0.03	3.188GlnLeu: 3.188 ± 0.042	0.798GlnMet: 0.798 ± 0.025	0.732GlnAsn: 0.732 ± 0.025	1.786GlnPro: 1.786 ± 0.04	1.099GlnGln: 1.099 ± 0.036	2.542GlnArg: 2.542 ± 0.049	1.701GlnSer: 1.701 ± 0.04	1.537GlnThr: 1.537 ± 0.035	2.07GlnVal: 2.07 ± 0.046	0.38GlnTrp: 0.38 ± 0.016	0.617GlnTyr: 0.617 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	9.137ArgAla: 9.137 ± 0.101	0.572ArgCys: 0.572 ± 0.022	4.558ArgAsp: 4.558 ± 0.066	4.014ArgGlu: 4.014 ± 0.064	3.111ArgPhe: 3.111 ± 0.05	5.251ArgGly: 5.251 ± 0.073	1.808ArgHis: 1.808 ± 0.041	4.443ArgIle: 4.443 ± 0.058	2.209ArgLys: 2.209 ± 0.045	8.341ArgLeu: 8.341 ± 0.092	1.989ArgMet: 1.989 ± 0.04	1.876ArgAsn: 1.876 ± 0.037	3.848ArgPro: 3.848 ± 0.057	2.525ArgGln: 2.525 ± 0.049	6.272ArgArg: 6.272 ± 0.096	4.03ArgSer: 4.03 ± 0.061	3.616ArgThr: 3.616 ± 0.058	4.744ArgVal: 4.744 ± 0.061	1.272ArgTrp: 1.272 ± 0.032	2.005ArgTyr: 2.005 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	6.543SerAla: 6.543 ± 0.074	0.438SerCys: 0.438 ± 0.018	3.158SerAsp: 3.158 ± 0.049	2.656SerGlu: 2.656 ± 0.049	2.289SerPhe: 2.289 ± 0.042	5.386SerGly: 5.386 ± 0.079	1.043SerHis: 1.043 ± 0.028	3.002SerIle: 3.002 ± 0.051	1.556SerLys: 1.556 ± 0.04	5.226SerLeu: 5.226 ± 0.065	1.279SerMet: 1.279 ± 0.033	1.349SerAsn: 1.349 ± 0.034	2.872SerPro: 2.872 ± 0.051	1.53SerGln: 1.53 ± 0.034	3.599SerArg: 3.599 ± 0.06	2.868SerSer: 2.868 ± 0.063	2.68SerThr: 2.68 ± 0.062	3.652SerVal: 3.652 ± 0.055	0.842SerTrp: 0.842 ± 0.029	1.422SerTyr: 1.422 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	6.405ThrAla: 6.405 ± 0.077	0.365ThrCys: 0.365 ± 0.015	2.862ThrAsp: 2.862 ± 0.051	2.322ThrGlu: 2.322 ± 0.042	1.619ThrPhe: 1.619 ± 0.035	5.26ThrGly: 5.26 ± 0.081	0.934ThrHis: 0.934 ± 0.03	3.208ThrIle: 3.208 ± 0.046	1.317ThrLys: 1.317 ± 0.037	5.547ThrLeu: 5.547 ± 0.077	1.221ThrMet: 1.221 ± 0.031	1.24ThrAsn: 1.24 ± 0.034	3.203ThrPro: 3.203 ± 0.054	1.335ThrGln: 1.335 ± 0.034	3.492ThrArg: 3.492 ± 0.051	2.7ThrSer: 2.7 ± 0.052	2.747ThrThr: 2.747 ± 0.051	3.895ThrVal: 3.895 ± 0.055	0.535ThrTrp: 0.535 ± 0.02	1.128ThrTyr: 1.128 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	9.362ValAla: 9.362 ± 0.08	0.538ValCys: 0.538 ± 0.022	4.377ValAsp: 4.377 ± 0.058	4.469ValGlu: 4.469 ± 0.065	2.027ValPhe: 2.027 ± 0.047	5.46ValGly: 5.46 ± 0.073	1.287ValHis: 1.287 ± 0.029	3.841ValIle: 3.841 ± 0.059	2.033ValLys: 2.033 ± 0.046	6.11ValLeu: 6.11 ± 0.077	1.686ValMet: 1.686 ± 0.038	1.845ValAsn: 1.845 ± 0.045	3.603ValPro: 3.603 ± 0.05	1.975ValGln: 1.975 ± 0.043	5.087ValArg: 5.087 ± 0.061	3.952ValSer: 3.952 ± 0.058	4.219ValThr: 4.219 ± 0.061	5.032ValVal: 5.032 ± 0.075	0.805ValTrp: 0.805 ± 0.022	1.36ValTyr: 1.36 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.353TrpAla: 1.353 ± 0.03	0.119TrpCys: 0.119 ± 0.011	0.688TrpAsp: 0.688 ± 0.022	0.586TrpGlu: 0.586 ± 0.023	0.532TrpPhe: 0.532 ± 0.023	0.895TrpGly: 0.895 ± 0.028	0.333TrpHis: 0.333 ± 0.016	0.78TrpIle: 0.78 ± 0.025	0.484TrpLys: 0.484 ± 0.02	1.741TrpLeu: 1.741 ± 0.042	0.347TrpMet: 0.347 ± 0.016	0.426TrpAsn: 0.426 ± 0.017	0.71TrpPro: 0.71 ± 0.026	0.569TrpGln: 0.569 ± 0.025	1.401TrpArg: 1.401 ± 0.038	0.868TrpSer: 0.868 ± 0.024	0.843TrpThr: 0.843 ± 0.022	0.771TrpVal: 0.771 ± 0.023	0.234TrpTrp: 0.234 ± 0.014	0.267TrpTyr: 0.267 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.672TyrAla: 2.672 ± 0.053	0.245TyrCys: 0.245 ± 0.014	1.558TyrAsp: 1.558 ± 0.039	1.132TyrGlu: 1.132 ± 0.031	0.853TyrPhe: 0.853 ± 0.026	2.149TyrGly: 2.149 ± 0.05	0.508TyrHis: 0.508 ± 0.021	0.954TyrIle: 0.954 ± 0.028	0.619TyrLys: 0.619 ± 0.02	2.123TyrLeu: 2.123 ± 0.042	0.451TyrMet: 0.451 ± 0.019	0.596TyrAsn: 0.596 ± 0.02	0.997TyrPro: 0.997 ± 0.028	0.699TyrGln: 0.699 ± 0.022	1.997TyrArg: 1.997 ± 0.047	1.179TyrSer: 1.179 ± 0.039	1.016TyrThr: 1.016 ± 0.033	1.544TyrVal: 1.544 ± 0.038	0.336TyrTrp: 0.336 ± 0.017	0.644TyrTyr: 0.644 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4289 proteins (1272449 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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