Drosophila busckii rhabdovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; unclassified Rhabdoviridae

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140D8L2|A0A140D8L2_9RHAB Polymerase-associated protein OS=Drosophila busckii rhabdovirus OX=1802935 PE=4 SV=1
MM1 pKa = 7.38AHH3 pKa = 6.91SSSQQISPRR12 pKa = 11.84DD13 pKa = 3.73VQILTDD19 pKa = 3.46GHH21 pKa = 5.89SVVAQMKK28 pKa = 9.22GISSVGEE35 pKa = 4.07SEE37 pKa = 4.73IQDD40 pKa = 4.15LPWTEE45 pKa = 4.16STFGEE50 pKa = 4.42LLDD53 pKa = 3.58MYY55 pKa = 9.82GWKK58 pKa = 9.4VHH60 pKa = 6.92PEE62 pKa = 3.68DD63 pKa = 3.91TVINGAASSILNWYY77 pKa = 8.56MGGPTKK83 pKa = 10.83NLLQSIEE90 pKa = 4.08VVIAGMTVSGSGTPLITIKK109 pKa = 10.95DD110 pKa = 3.55RR111 pKa = 11.84GTKK114 pKa = 8.26STHH117 pKa = 4.96KK118 pKa = 9.22WPSIVIPKK126 pKa = 10.28RR127 pKa = 11.84LDD129 pKa = 3.41GDD131 pKa = 4.03QLAKK135 pKa = 9.94WKK137 pKa = 9.34PALEE141 pKa = 4.29NLDD144 pKa = 3.7QEE146 pKa = 4.75ISIAPPALPTSVEE159 pKa = 4.13GAEE162 pKa = 4.34TTRR165 pKa = 11.84MATSQTEE172 pKa = 3.63AGKK175 pKa = 9.6WYY177 pKa = 10.43DD178 pKa = 4.33FMWDD182 pKa = 3.73GLNTAITAEE191 pKa = 4.28DD192 pKa = 3.78PKK194 pKa = 11.16ADD196 pKa = 4.39EE197 pKa = 4.3IRR199 pKa = 11.84KK200 pKa = 9.14KK201 pKa = 10.38RR202 pKa = 11.84QFIINYY208 pKa = 8.66VGYY211 pKa = 10.18IGLNLLRR218 pKa = 11.84GVAKK222 pKa = 10.76GSTTVIRR229 pKa = 11.84ALEE232 pKa = 4.14KK233 pKa = 10.88NLVEE237 pKa = 5.25RR238 pKa = 11.84IPHH241 pKa = 5.66FWPTEE246 pKa = 3.96FQVPPPPMGAQSAIQEE262 pKa = 4.22IEE264 pKa = 3.88NMLEE268 pKa = 4.16RR269 pKa = 11.84GSEE272 pKa = 4.04GCRR275 pKa = 11.84RR276 pKa = 11.84LIVNMIPDD284 pKa = 4.02TLSTEE289 pKa = 4.08PAKK292 pKa = 10.73KK293 pKa = 10.07GILMSGCMKK302 pKa = 10.49SLSDD306 pKa = 3.56SGLGALHH313 pKa = 6.65WVYY316 pKa = 10.62EE317 pKa = 4.03AAAYY321 pKa = 7.66LTVEE325 pKa = 4.87PGDD328 pKa = 3.83LMEE331 pKa = 4.24TMYY334 pKa = 10.63YY335 pKa = 10.82GPFIASSQEE344 pKa = 3.77VAGFLSSMHH353 pKa = 6.44GLPKK357 pKa = 8.05EE358 pKa = 4.37WKK360 pKa = 7.15WARR363 pKa = 11.84VINEE367 pKa = 4.15GYY369 pKa = 9.83ISKK372 pKa = 10.78LSVRR376 pKa = 11.84NNKK379 pKa = 9.9KK380 pKa = 10.17YY381 pKa = 10.68VATLVALSCGMSPDD395 pKa = 3.56SPIWAIPSLAINNLIKK411 pKa = 10.6RR412 pKa = 11.84EE413 pKa = 4.02ALDD416 pKa = 3.61NAQAIYY422 pKa = 10.96SEE424 pKa = 4.37LTDD427 pKa = 3.44TRR429 pKa = 11.84NYY431 pKa = 10.62AAGSAKK437 pKa = 10.11SAAVVAALSRR447 pKa = 11.84IRR449 pKa = 11.84TRR451 pKa = 11.84DD452 pKa = 3.53PLEE455 pKa = 4.77GIPTAVPCAPVSKK468 pKa = 10.47CPPTSDD474 pKa = 3.21GFGRR478 pKa = 11.84EE479 pKa = 4.1YY480 pKa = 11.41

Molecular weight: 52.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140D8L4|A0A140D8L4_9RHAB Glycoprotein OS=Drosophila busckii rhabdovirus OX=1802935 GN=G PE=4 SV=1
MM1 pKa = 7.56AKK3 pKa = 9.92INHH6 pKa = 7.11DD7 pKa = 3.47YY8 pKa = 10.92HH9 pKa = 6.72NRR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 6.07FKK15 pKa = 8.37TTVQVDD21 pKa = 3.19ILLWGTSEE29 pKa = 5.16IDD31 pKa = 3.36PEE33 pKa = 4.46LLKK36 pKa = 11.06KK37 pKa = 9.82MCACIIWEE45 pKa = 4.7GYY47 pKa = 9.43KK48 pKa = 10.84GIVEE52 pKa = 4.81DD53 pKa = 4.37PMMWKK58 pKa = 9.35QASQALYY65 pKa = 10.8SKK67 pKa = 10.45IRR69 pKa = 11.84DD70 pKa = 3.43KK71 pKa = 11.49SRR73 pKa = 11.84WSPVLSDD80 pKa = 4.36LNHH83 pKa = 6.27HH84 pKa = 6.65AVLEE88 pKa = 4.28ATWFSLTPRR97 pKa = 11.84IIAFPDD103 pKa = 3.35DD104 pKa = 3.94HH105 pKa = 6.79KK106 pKa = 11.47VSYY109 pKa = 10.89SRR111 pKa = 11.84LFNFRR116 pKa = 11.84APGGKK121 pKa = 8.87ILSVDD126 pKa = 3.67YY127 pKa = 9.93KK128 pKa = 10.49IHH130 pKa = 6.5MIHH133 pKa = 6.67NIWDD137 pKa = 3.25KK138 pKa = 11.29RR139 pKa = 11.84EE140 pKa = 3.84YY141 pKa = 10.97GNLVMRR147 pKa = 11.84GYY149 pKa = 9.0TCLEE153 pKa = 3.85QLGLTPRR160 pKa = 11.84IGGVISLAIEE170 pKa = 4.02KK171 pKa = 10.0MIGTVVLDD179 pKa = 4.0PYY181 pKa = 11.64KK182 pKa = 10.05MVSLMEE188 pKa = 4.08MAPLIEE194 pKa = 4.12QTEE197 pKa = 4.65VIDD200 pKa = 4.1TPSGPKK206 pKa = 9.56LKK208 pKa = 10.52KK209 pKa = 10.46GFLSQFRR216 pKa = 11.84KK217 pKa = 10.2

Molecular weight: 25.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4214

111

2327

702.3

79.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.865 ± 0.696

1.732 ± 0.21

4.722 ± 0.172

5.956 ± 0.379

4.058 ± 0.669

5.434 ± 0.52

2.492 ± 0.222

7.143 ± 0.194

5.766 ± 0.465

9.991 ± 0.627

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.539 ± 0.244

4.746 ± 0.337

6.407 ± 0.401

3.821 ± 0.401

5.173 ± 0.19

8.353 ± 0.416

6.122 ± 0.274

5.15 ± 0.308

1.875 ± 0.191

3.654 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski