Hydrogenophaga crassostreae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Hydrogenophaga

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4456 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A162W3I5|A0A162W3I5_9BURK Peptidase OS=Hydrogenophaga crassostreae OX=1763535 GN=LPB072_17715 PE=4 SV=1
MM1 pKa = 7.85SDD3 pKa = 3.32TPTTPPLTPSIEE15 pKa = 4.19DD16 pKa = 3.99AEE18 pKa = 4.5PSLPCVMVFNANDD31 pKa = 3.66PSGAGGLAADD41 pKa = 4.8LSAMGSASAHH51 pKa = 4.99VLAVVTGTYY60 pKa = 10.49VRR62 pKa = 11.84DD63 pKa = 3.34TTEE66 pKa = 3.67IHH68 pKa = 6.9DD69 pKa = 4.58HH70 pKa = 6.75FPFDD74 pKa = 4.56DD75 pKa = 4.03EE76 pKa = 5.37AVTDD80 pKa = 3.77QARR83 pKa = 11.84CALEE87 pKa = 4.41DD88 pKa = 3.59MPVQAFKK95 pKa = 11.36VGFVGSAEE103 pKa = 4.03NLSAIAAIATDD114 pKa = 3.71YY115 pKa = 11.46TEE117 pKa = 4.72IPVITYY123 pKa = 9.03MPDD126 pKa = 3.39LSWWDD131 pKa = 3.38EE132 pKa = 4.13VEE134 pKa = 3.56IDD136 pKa = 4.56NYY138 pKa = 11.33LDD140 pKa = 3.5ACAEE144 pKa = 3.97LMLPQTTVLVGNHH157 pKa = 5.01STLCRR162 pKa = 11.84WLLPDD167 pKa = 3.75WDD169 pKa = 4.38GDD171 pKa = 3.81KK172 pKa = 10.74PPPPRR177 pKa = 11.84EE178 pKa = 3.95VARR181 pKa = 11.84AAAVHH186 pKa = 5.84GVPYY190 pKa = 10.28TLVTGFNAADD200 pKa = 3.5QYY202 pKa = 11.67LEE204 pKa = 4.05SHH206 pKa = 6.85LASPEE211 pKa = 4.17TVLATARR218 pKa = 11.84YY219 pKa = 8.48EE220 pKa = 3.99RR221 pKa = 11.84FEE223 pKa = 4.21ATFSGAGDD231 pKa = 3.74TLSAALCALIANGMDD246 pKa = 3.57LQAACAEE253 pKa = 4.08ALTYY257 pKa = 10.72LDD259 pKa = 3.41QCLDD263 pKa = 3.39AGFQPGMGHH272 pKa = 7.23AVPDD276 pKa = 3.57RR277 pKa = 11.84MFWAHH282 pKa = 6.97SDD284 pKa = 3.93DD285 pKa = 3.89EE286 pKa = 5.57DD287 pKa = 3.92EE288 pKa = 5.16VEE290 pKa = 4.27PSSKK294 pKa = 9.24NTLGTEE300 pKa = 4.08DD301 pKa = 4.68FPLDD305 pKa = 3.87TTKK308 pKa = 10.67HH309 pKa = 4.89

Molecular weight: 33.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A163C5F1|A0A163C5F1_9BURK Histidine kinase OS=Hydrogenophaga crassostreae OX=1763535 GN=LPB072_21730 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.17QPSKK9 pKa = 9.15IKK11 pKa = 10.36RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.98GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4456

0

4456

1430289

27

4885

321.0

34.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.16 ± 0.046

0.948 ± 0.013

5.141 ± 0.034

5.324 ± 0.031

3.627 ± 0.02

8.306 ± 0.034

2.322 ± 0.017

4.336 ± 0.025

3.507 ± 0.033

10.668 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.756 ± 0.02

2.867 ± 0.021

5.188 ± 0.031

4.093 ± 0.024

6.258 ± 0.036

5.766 ± 0.031

5.325 ± 0.044

7.703 ± 0.035

1.565 ± 0.018

2.14 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski