Croceivirga radicis
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3064 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V6LPX4|A0A1V6LPX4_9FLAO SAM-dependent methyltransferase OS=Croceivirga radicis OX=1929488 GN=BUL40_12190 PE=4 SV=1
MM1 pKa = 7.14 TKK3 pKa = 10.23 FRR5 pKa = 11.84 ICVIHH10 pKa = 6.99 CIDD13 pKa = 3.62 YY14 pKa = 10.87 LRR16 pKa = 11.84 RR17 pKa = 11.84 TATFSYY23 pKa = 10.62 LILAFVFSIQQLSAQTTFTEE43 pKa = 4.67 SAASYY48 pKa = 10.19 NLNIGQSKK56 pKa = 10.86 DD57 pKa = 3.5 GGHH60 pKa = 6.62 AWADD64 pKa = 3.36 FDD66 pKa = 6.27 LDD68 pKa = 3.33 GDD70 pKa = 3.83 QDD72 pKa = 4.05 VLVLVNSTSQKK83 pKa = 10.21 ARR85 pKa = 11.84 LMRR88 pKa = 11.84 NNGDD92 pKa = 3.5 GTFTNVQTTLAPGILNARR110 pKa = 11.84 AEE112 pKa = 4.22 RR113 pKa = 11.84 QAAWGDD119 pKa = 3.68 LNNDD123 pKa = 3.09 GRR125 pKa = 11.84 PDD127 pKa = 3.69 FMITSSGNSGGNPSPPALQIFLQNLDD153 pKa = 3.69 GTFGDD158 pKa = 4.68 ALGGTNPITVGRR170 pKa = 11.84 NGHH173 pKa = 6.55 TITINPMNAEE183 pKa = 3.88 GAGFFDD189 pKa = 5.29 FEE191 pKa = 5.45 GDD193 pKa = 3.18 GDD195 pKa = 4.44 LDD197 pKa = 4.12 IFFDD201 pKa = 3.52 SHH203 pKa = 7.59 NYY205 pKa = 9.73 GIEE208 pKa = 3.78 ILRR211 pKa = 11.84 NNYY214 pKa = 8.86 IDD216 pKa = 3.84 HH217 pKa = 6.74 TNSTVTNPTAALLFSHH233 pKa = 6.64 ITTGNGNGVVNFGLNQFATDD253 pKa = 3.59 GDD255 pKa = 4.38 YY256 pKa = 10.12 GTAADD261 pKa = 4.69 ANDD264 pKa = 5.01 DD265 pKa = 3.6 GWVDD269 pKa = 2.52 IFMRR273 pKa = 11.84 KK274 pKa = 8.61 RR275 pKa = 11.84 DD276 pKa = 3.74 EE277 pKa = 4.48 NDD279 pKa = 2.67 FFLNQGGIFTNGSDD293 pKa = 4.22 LAQAANGNKK302 pKa = 9.6 GGNGLWDD309 pKa = 4.35 LDD311 pKa = 3.81 NDD313 pKa = 4.14 GDD315 pKa = 5.06 LDD317 pKa = 4.86 AVWTEE322 pKa = 3.76 NGLTQIYY329 pKa = 9.96 RR330 pKa = 11.84 NDD332 pKa = 3.5 GSGIWTALGTGVFPGLPQPNNSNNGNSSNRR362 pKa = 11.84 IDD364 pKa = 3.82 ALAGGDD370 pKa = 3.72 IDD372 pKa = 4.66 NDD374 pKa = 3.56 GDD376 pKa = 3.96 IDD378 pKa = 3.8 ILLVGQSRR386 pKa = 11.84 SYY388 pKa = 11.44 LYY390 pKa = 10.31 INQLNSPTPAPGVIGSGSPMNFSLDD415 pKa = 3.34 PEE417 pKa = 4.62 TFNTNDD423 pKa = 3.64 GEE425 pKa = 4.61 GTTMVDD431 pKa = 3.05 VDD433 pKa = 5.18 DD434 pKa = 6.66 DD435 pKa = 4.8 GDD437 pKa = 4.09 LDD439 pKa = 3.71 IYY441 pKa = 11.01 MNINGSGNQLWINNLAAANRR461 pKa = 11.84 NNHH464 pKa = 5.71 LMVNVIEE471 pKa = 5.01 DD472 pKa = 3.62 RR473 pKa = 11.84 ASNGDD478 pKa = 3.16 TGGFAGRR485 pKa = 11.84 IALGTNVLIRR495 pKa = 11.84 DD496 pKa = 3.73 CSGNIVSGLRR506 pKa = 11.84 QVNGVYY512 pKa = 10.51 GHH514 pKa = 6.51 GTQQPEE520 pKa = 4.33 TVHH523 pKa = 6.64 FGLPLGEE530 pKa = 4.15 NEE532 pKa = 4.18 TYY534 pKa = 10.13 IIEE537 pKa = 4.04 VRR539 pKa = 11.84 YY540 pKa = 9.64 PNFNDD545 pKa = 3.45 PEE547 pKa = 4.11 EE548 pKa = 5.28 GITRR552 pKa = 11.84 LIATGVAQPSTIAGTNHH569 pKa = 6.02 YY570 pKa = 11.07 NLTTTNAEE578 pKa = 4.32 TIQNLNAPEE587 pKa = 5.24 AEE589 pKa = 4.76 DD590 pKa = 4.07 DD591 pKa = 4.73 LVTIPYY597 pKa = 9.09 GNSVSIQINLFTNDD611 pKa = 3.92 SEE613 pKa = 4.64 PDD615 pKa = 3.43 GEE617 pKa = 4.41 NFFIEE622 pKa = 5.71 SITQPTVGSVTIDD635 pKa = 3.35 DD636 pKa = 4.53 ANNGLVTYY644 pKa = 8.05 TYY646 pKa = 11.3 NNAAPFPGSTSFTYY660 pKa = 10.06 TIVDD664 pKa = 3.42 DD665 pKa = 4.54 TYY667 pKa = 11.38 NICAAQGKK675 pKa = 9.79 SDD677 pKa = 3.6 TATVTVYY684 pKa = 9.86 EE685 pKa = 4.42 PCSDD689 pKa = 3.58 PTGVDD694 pKa = 3.01 TDD696 pKa = 3.87 GDD698 pKa = 4.69 GINDD702 pKa = 3.23 ICDD705 pKa = 3.47 YY706 pKa = 11.71 DD707 pKa = 4.59 DD708 pKa = 5.9 DD709 pKa = 5.38 NDD711 pKa = 5.0 GISDD715 pKa = 4.27 FDD717 pKa = 3.83 EE718 pKa = 4.56 QQCSTTNPGVLGVPTTVLGGTAVDD742 pKa = 5.36 EE743 pKa = 5.24 IYY745 pKa = 10.77 TDD747 pKa = 4.07 YY748 pKa = 11.45 NGYY751 pKa = 9.22 WYY753 pKa = 10.87 SSTASINSIEE763 pKa = 4.44 PNLSHH768 pKa = 7.02 NLLAFGSGGNLYY780 pKa = 9.14 STGVVNTNMLDD791 pKa = 3.33 TDD793 pKa = 4.0 SNGRR797 pKa = 11.84 YY798 pKa = 9.59 DD799 pKa = 5.08 AVDD802 pKa = 3.49 TNGDD806 pKa = 2.98 GSGNINITEE815 pKa = 4.16 MEE817 pKa = 3.78 WRR819 pKa = 11.84 AFRR822 pKa = 11.84 PNSNITNKK830 pKa = 9.75 VALEE834 pKa = 3.97 SALNDD839 pKa = 3.65 GTLGSALGSTVIANLATDD857 pKa = 4.45 PLNPLLTNGPRR868 pKa = 11.84 GLDD871 pKa = 3.4 LGTGIANVFDD881 pKa = 3.73 SWFFNISTIDD891 pKa = 3.14 IAAVNDD897 pKa = 4.21 GLPDD901 pKa = 3.48 ILITQVAQISSTVDD915 pKa = 2.84 HH916 pKa = 6.96 NIAFFDD922 pKa = 3.66 AAGNPVGNQVRR933 pKa = 11.84 VTSLGGGEE941 pKa = 4.13 LSSVIGAQRR950 pKa = 11.84 YY951 pKa = 8.4 DD952 pKa = 3.35 VYY954 pKa = 10.3 QTNGSSWQVNVTKK967 pKa = 10.43 NIRR970 pKa = 11.84 MATLEE975 pKa = 4.04 LSEE978 pKa = 5.04 FGLDD982 pKa = 3.24 ASNIGNVALMRR993 pKa = 11.84 LTLNNSADD1001 pKa = 4.02 TAFLAYY1007 pKa = 11.0 NMDD1010 pKa = 3.83 SFGEE1014 pKa = 4.3 FCTDD1018 pKa = 4.17 LDD1020 pKa = 3.97 TDD1022 pKa = 3.65 GDD1024 pKa = 4.59 GIVDD1028 pKa = 4.55 RR1029 pKa = 11.84 LDD1031 pKa = 4.13 LDD1033 pKa = 3.67 SDD1035 pKa = 3.62 NDD1037 pKa = 4.44 GIYY1040 pKa = 10.89 DD1041 pKa = 3.76 VVEE1044 pKa = 4.41 AGHH1047 pKa = 6.59 NLTHH1051 pKa = 6.32 TNGRR1055 pKa = 11.84 LTGAVGADD1063 pKa = 4.07 GIIDD1067 pKa = 4.09 ALQTNPNNQTTTYY1080 pKa = 10.32 IVADD1084 pKa = 4.0 TNGDD1088 pKa = 4.14 GIPDD1092 pKa = 4.45 FKK1094 pKa = 11.52 NLDD1097 pKa = 3.71 SDD1099 pKa = 4.71 GDD1101 pKa = 3.83 GCTDD1105 pKa = 3.15 VNEE1108 pKa = 5.32 AGITDD1113 pKa = 3.95 NNDD1116 pKa = 2.93 DD1117 pKa = 4.73 GYY1119 pKa = 11.52 LGDD1122 pKa = 4.12 IPLTVSLDD1130 pKa = 3.65 GVVTSGADD1138 pKa = 3.6 GYY1140 pKa = 6.54 TTPVDD1145 pKa = 3.46 ADD1147 pKa = 3.57 GNGIYY1152 pKa = 10.19 DD1153 pKa = 3.79 YY1154 pKa = 10.27 TEE1156 pKa = 3.82 VGAAPTITDD1165 pKa = 3.34 QPTNTTICPGCSGTITANFANADD1188 pKa = 3.71 GYY1190 pKa = 10.44 QWQLFNGSTWEE1201 pKa = 3.85 NLVNGGLYY1209 pKa = 10.7 GGTTTQTLTITNPTSAEE1226 pKa = 3.8 HH1227 pKa = 6.34 GNTYY1231 pKa = 10.3 RR1232 pKa = 11.84 IVGSSTTFVCRR1243 pKa = 11.84 NAISNPVVLSVRR1255 pKa = 11.84 INTVITNRR1263 pKa = 11.84 RR1264 pKa = 11.84 ITYY1267 pKa = 10.09 RR1268 pKa = 11.84 VDD1270 pKa = 3.17 PKK1272 pKa = 11.36
Molecular weight: 135.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.667
IPC2_protein 3.719
IPC_protein 3.783
Toseland 3.541
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.605
Grimsley 3.439
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.202
Thurlkill 3.592
EMBOSS 3.757
Sillero 3.91
Patrickios 1.303
IPC_peptide 3.783
IPC2_peptide 3.884
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|A0A1V6LTL2|A0A1V6LTL2_9FLAO Bifunctional alpha alpha-trehalose-phosphate synthase (UDP-forming)/trehalose-phosphatase OS=Croceivirga radicis OX=1929488 GN=BUL40_06645 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.13 RR10 pKa = 11.84 KK11 pKa = 9.48 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.49 HH16 pKa = 3.94 GFRR19 pKa = 11.84 EE20 pKa = 4.19 RR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 8.93 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.08 GRR39 pKa = 11.84 KK40 pKa = 8.3 KK41 pKa = 9.83 LTVSSEE47 pKa = 3.9 PRR49 pKa = 11.84 HH50 pKa = 5.77 KK51 pKa = 10.61 KK52 pKa = 9.84
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3064
0
3064
1106336
38
4776
361.1
40.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.085 ± 0.045
0.704 ± 0.017
5.619 ± 0.044
6.501 ± 0.041
5.073 ± 0.03
6.816 ± 0.052
1.75 ± 0.024
7.09 ± 0.041
7.355 ± 0.073
9.654 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.132 ± 0.022
6.051 ± 0.04
3.604 ± 0.029
3.582 ± 0.025
3.398 ± 0.028
5.868 ± 0.031
6.093 ± 0.064
6.422 ± 0.033
1.138 ± 0.016
4.066 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here