Hubei sobemo-like virus 22

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFA4|A0A1L3KFA4_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 22 OX=1923208 PE=4 SV=1
MM1 pKa = 7.75LEE3 pKa = 3.95TAYY6 pKa = 10.22EE7 pKa = 4.31SYY9 pKa = 8.83KK10 pKa = 8.89TATWAIPDD18 pKa = 3.98DD19 pKa = 4.09FLSRR23 pKa = 11.84EE24 pKa = 3.98HH25 pKa = 6.39YY26 pKa = 10.75LRR28 pKa = 11.84TLARR32 pKa = 11.84LDD34 pKa = 3.88LRR36 pKa = 11.84SSPGYY41 pKa = 9.67PYY43 pKa = 10.23MLSAPTNRR51 pKa = 11.84DD52 pKa = 2.43WFRR55 pKa = 11.84VSKK58 pKa = 10.92DD59 pKa = 3.46GTWDD63 pKa = 3.25TGRR66 pKa = 11.84VDD68 pKa = 3.82QMWKK72 pKa = 9.82IVQGKK77 pKa = 8.88LAGQGADD84 pKa = 4.08PIRR87 pKa = 11.84IFIKK91 pKa = 10.43PEE93 pKa = 3.14PHH95 pKa = 5.86KK96 pKa = 10.49RR97 pKa = 11.84KK98 pKa = 10.1KK99 pKa = 9.95IEE101 pKa = 3.68EE102 pKa = 3.95GRR104 pKa = 11.84FRR106 pKa = 11.84IISSVSVADD115 pKa = 3.79QIVDD119 pKa = 3.13HH120 pKa = 6.49MLFAEE125 pKa = 4.88CNDD128 pKa = 3.27ALINNWQFVPNKK140 pKa = 10.37AGWTHH145 pKa = 7.15LMGGWKK151 pKa = 9.25MIPAASWIAADD162 pKa = 3.99KK163 pKa = 11.04SAWDD167 pKa = 3.33WTAKK171 pKa = 9.88YY172 pKa = 9.75WLFEE176 pKa = 4.16LVLSLRR182 pKa = 11.84IMLCSNMSPEE192 pKa = 3.65WMEE195 pKa = 3.74RR196 pKa = 11.84AVRR199 pKa = 11.84RR200 pKa = 11.84YY201 pKa = 9.9RR202 pKa = 11.84EE203 pKa = 4.31LFEE206 pKa = 5.3HH207 pKa = 7.0PLFITSGGQLLRR219 pKa = 11.84QKK221 pKa = 9.29HH222 pKa = 4.39TGIMKK227 pKa = 9.93SGCVNTISDD236 pKa = 4.21NSIMQYY242 pKa = 10.44FLHH245 pKa = 6.84LRR247 pKa = 11.84VCLEE251 pKa = 4.0LGIEE255 pKa = 4.11PSTLFAMGDD264 pKa = 3.5DD265 pKa = 3.95TLQEE269 pKa = 4.08PVVGAEE275 pKa = 3.78YY276 pKa = 10.66FEE278 pKa = 4.61YY279 pKa = 10.62LGQFCRR285 pKa = 11.84LKK287 pKa = 10.37DD288 pKa = 3.3WQRR291 pKa = 11.84ANEE294 pKa = 3.8FAGFRR299 pKa = 11.84FHH301 pKa = 7.12GRR303 pKa = 11.84TVEE306 pKa = 3.67PLYY309 pKa = 10.6KK310 pKa = 10.35GKK312 pKa = 9.54HH313 pKa = 5.14AFNLLYY319 pKa = 9.64MDD321 pKa = 5.76PDD323 pKa = 3.86NLSQMSDD330 pKa = 3.02AYY332 pKa = 10.99SLLYY336 pKa = 10.12HH337 pKa = 7.06RR338 pKa = 11.84SRR340 pKa = 11.84FGGWVKK346 pKa = 10.86DD347 pKa = 3.52IFNKK351 pKa = 9.91MGVGVRR357 pKa = 11.84SNEE360 pKa = 3.74WFDD363 pKa = 4.64LVFDD367 pKa = 5.17GYY369 pKa = 11.8

Molecular weight: 42.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFA4|A0A1L3KFA4_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 22 OX=1923208 PE=4 SV=1
MM1 pKa = 7.87KK2 pKa = 10.07YY3 pKa = 10.58GKK5 pKa = 10.02SFSLAVAGAVGGRR18 pKa = 11.84YY19 pKa = 8.82VFIPVVKK26 pKa = 10.22LAWNWVVFILWTYY39 pKa = 11.24NPVFLCGSALEE50 pKa = 4.2EE51 pKa = 4.49AVLSLNPEE59 pKa = 3.77WPAPPKK65 pKa = 9.57PDD67 pKa = 3.54PPIISVEE74 pKa = 3.85MAKK77 pKa = 10.07IGFSQLRR84 pKa = 11.84RR85 pKa = 11.84LVNWIEE91 pKa = 3.81EE92 pKa = 3.83FSIYY96 pKa = 10.42DD97 pKa = 3.15ILAYY101 pKa = 9.0FMCGIIVLILISKK114 pKa = 7.71TYY116 pKa = 10.86QLVKK120 pKa = 10.68DD121 pKa = 3.83STRR124 pKa = 11.84QAVLRR129 pKa = 11.84ARR131 pKa = 11.84GVSFEE136 pKa = 4.05SMRR139 pKa = 11.84AGSVLVKK146 pKa = 10.87GEE148 pKa = 3.76IPKK151 pKa = 10.24YY152 pKa = 9.41QVAVLRR158 pKa = 11.84AGLLRR163 pKa = 11.84DD164 pKa = 3.29GHH166 pKa = 6.98VGYY169 pKa = 10.71GIRR172 pKa = 11.84VGNYY176 pKa = 8.24LVAPHH181 pKa = 6.56HH182 pKa = 6.39VVTDD186 pKa = 3.62AGEE189 pKa = 4.0EE190 pKa = 4.31VILSSKK196 pKa = 9.07LAKK199 pKa = 10.65VMMPPASAIRR209 pKa = 11.84SRR211 pKa = 11.84VVTDD215 pKa = 4.86LVYY218 pKa = 10.85VWLPEE223 pKa = 4.25SFWVKK228 pKa = 10.55LGVPSAKK235 pKa = 9.8FAQTTEE241 pKa = 4.18NPLATCAGPEE251 pKa = 4.35GVSSGLLRR259 pKa = 11.84KK260 pKa = 9.73SQVLGLVNYY269 pKa = 9.82FGSTLPGFSGAAYY282 pKa = 8.26EE283 pKa = 4.51AAGHH287 pKa = 6.17CMGIHH292 pKa = 6.44IGAVAGNNMGISAVVVVKK310 pKa = 10.2EE311 pKa = 3.81LAHH314 pKa = 6.66LVRR317 pKa = 11.84QEE319 pKa = 3.93SSQDD323 pKa = 3.21VAASYY328 pKa = 10.0SQQLGAPKK336 pKa = 9.93KK337 pKa = 9.85QMWGAIQIGEE347 pKa = 3.98IADD350 pKa = 4.4RR351 pKa = 11.84NWGDD355 pKa = 3.98DD356 pKa = 3.22AWHH359 pKa = 7.05LEE361 pKa = 3.67QSLDD365 pKa = 3.42YY366 pKa = 11.07DD367 pKa = 3.91QILSFDD373 pKa = 4.64DD374 pKa = 3.91EE375 pKa = 5.01SKK377 pKa = 10.63PRR379 pKa = 11.84PAVPQAVLDD388 pKa = 3.9FQPTIKK394 pKa = 9.76LTQHH398 pKa = 6.22SDD400 pKa = 2.92SAAEE404 pKa = 4.1VSYY407 pKa = 11.23SLYY410 pKa = 9.84PSKK413 pKa = 11.11SEE415 pKa = 4.06AGVEE419 pKa = 3.84ARR421 pKa = 11.84LTRR424 pKa = 11.84LEE426 pKa = 4.12EE427 pKa = 3.81RR428 pKa = 11.84CVRR431 pKa = 11.84LEE433 pKa = 3.81QAVLLLQEE441 pKa = 4.83GKK443 pKa = 11.0GKK445 pKa = 10.0FPCDD449 pKa = 3.35SCPVVCRR456 pKa = 11.84TQEE459 pKa = 3.81KK460 pKa = 10.42LDD462 pKa = 3.53AHH464 pKa = 6.82VGSAHH469 pKa = 5.39VSRR472 pKa = 11.84PRR474 pKa = 11.84EE475 pKa = 3.84YY476 pKa = 9.79MDD478 pKa = 5.27CSVCGVKK485 pKa = 10.2VRR487 pKa = 11.84SDD489 pKa = 3.31RR490 pKa = 11.84LEE492 pKa = 3.79KK493 pKa = 10.45HH494 pKa = 5.69ALKK497 pKa = 10.48CIRR500 pKa = 11.84SNPVKK505 pKa = 10.33PEE507 pKa = 3.83SAVPCDD513 pKa = 3.2ARR515 pKa = 11.84VSVKK519 pKa = 8.95TDD521 pKa = 3.02RR522 pKa = 11.84TVPFLGKK529 pKa = 9.96RR530 pKa = 11.84SRR532 pKa = 11.84PVKK535 pKa = 10.26LRR537 pKa = 11.84PSKK540 pKa = 9.87NTSTTRR546 pKa = 11.84ATNRR550 pKa = 11.84QSQLLQEE557 pKa = 4.57LQSSVRR563 pKa = 11.84EE564 pKa = 4.09FQRR567 pKa = 11.84VCAMFSSAPQQATNGQNLAPARR589 pKa = 11.84SS590 pKa = 3.47

Molecular weight: 64.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

959

369

590

479.5

53.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.238 ± 0.919

1.877 ± 0.277

4.692 ± 0.817

5.422 ± 0.001

4.067 ± 0.861

6.882 ± 0.087

2.19 ± 0.276

4.588 ± 0.297

5.214 ± 0.034

9.281 ± 0.108

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.711 ± 0.718

2.92 ± 0.32

5.214 ± 0.609

4.171 ± 0.487

6.361 ± 0.22

8.133 ± 0.864

3.65 ± 0.22

8.446 ± 1.892

2.607 ± 0.773

3.337 ± 0.386
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski