Pseudoalteromonas virus vB_PspP-H6/1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A191ZDJ4|A0A191ZDJ4_9CAUD Uncharacterized protein OS=Pseudoalteromonas virus vB_PspP-H6/1 OX=1852626 PE=4 SV=1
MM1 pKa = 7.53AVLTFTQADD10 pKa = 3.81GLYY13 pKa = 7.44TTDD16 pKa = 5.22VVAQTNDD23 pKa = 3.02FSVEE27 pKa = 3.94GNLLKK32 pKa = 10.47ADD34 pKa = 5.18SIPASNDD41 pKa = 2.42ARR43 pKa = 11.84ILFNSQSDD51 pKa = 3.85GTFSVDD57 pKa = 2.9VSTLSVVSNNVGLVFRR73 pKa = 11.84AVDD76 pKa = 3.32VDD78 pKa = 3.51NDD80 pKa = 3.62VVLFSGNKK88 pKa = 7.21EE89 pKa = 3.55WSGEE93 pKa = 4.01FTSFTFTGVDD103 pKa = 2.52AGTNVEE109 pKa = 4.33YY110 pKa = 10.72YY111 pKa = 10.74AYY113 pKa = 9.07GTGGGAGLQVGVTEE127 pKa = 4.38

Molecular weight: 13.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A191ZDG1|A0A191ZDG1_9CAUD Putative NinB-like recombinase OS=Pseudoalteromonas virus vB_PspP-H6/1 OX=1852626 PE=4 SV=1
MM1 pKa = 7.18QLTHH5 pKa = 6.85NIEE8 pKa = 4.32YY9 pKa = 10.58CPEE12 pKa = 4.11TGVCRR17 pKa = 11.84YY18 pKa = 7.34TKK20 pKa = 10.42RR21 pKa = 11.84SGKK24 pKa = 10.26KK25 pKa = 8.09KK26 pKa = 8.84TGDD29 pKa = 3.5VAGGRR34 pKa = 11.84YY35 pKa = 8.65KK36 pKa = 10.72AKK38 pKa = 10.03KK39 pKa = 9.35RR40 pKa = 11.84VYY42 pKa = 10.71LRR44 pKa = 11.84IFVNGQSTPLHH55 pKa = 5.38RR56 pKa = 11.84VIMKK60 pKa = 10.66AFGHH64 pKa = 6.78KK65 pKa = 9.71IGGMYY70 pKa = 9.04IDD72 pKa = 5.59HH73 pKa = 7.18INGDD77 pKa = 3.84SLDD80 pKa = 3.77NRR82 pKa = 11.84LCNLRR87 pKa = 11.84VIHH90 pKa = 6.4PSVSSLNLATQRR102 pKa = 11.84RR103 pKa = 11.84SKK105 pKa = 9.9TGVAGVTFVKK115 pKa = 10.63GKK117 pKa = 10.16FEE119 pKa = 4.66SRR121 pKa = 11.84VTRR124 pKa = 11.84NGKK127 pKa = 9.19PYY129 pKa = 9.6YY130 pKa = 10.05LGRR133 pKa = 11.84FEE135 pKa = 4.79TLGEE139 pKa = 4.12AKK141 pKa = 10.27NAQNEE146 pKa = 3.93IRR148 pKa = 11.84KK149 pKa = 9.31EE150 pKa = 3.91FGFHH154 pKa = 6.24INHH157 pKa = 5.86GRR159 pKa = 11.84KK160 pKa = 8.99VVEE163 pKa = 4.73PYY165 pKa = 10.93

Molecular weight: 18.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11418

45

1507

203.9

22.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.065 ± 0.421

1.068 ± 0.16

6.411 ± 0.331

6.691 ± 0.606

3.941 ± 0.249

7.979 ± 0.425

1.358 ± 0.183

5.579 ± 0.212

6.385 ± 0.552

7.12 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.706 ± 0.237

5.342 ± 0.271

3.153 ± 0.151

4.624 ± 0.558

4.545 ± 0.267

6.77 ± 0.357

5.649 ± 0.519

6.691 ± 0.251

1.401 ± 0.165

3.521 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski