Amino acid dipepetide frequency for Arthrobacter phage MediumFry

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.652AlaAla: 8.652 ± 1.149
0.398AlaCys: 0.398 ± 0.166
4.781AlaAsp: 4.781 ± 0.455
5.863AlaGlu: 5.863 ± 0.666
3.245AlaPhe: 3.245 ± 0.355
7.855AlaGly: 7.855 ± 1.334
1.821AlaHis: 1.821 ± 0.35
5.066AlaIle: 5.066 ± 0.53
5.635AlaLys: 5.635 ± 0.911
6.375AlaLeu: 6.375 ± 0.914
3.245AlaMet: 3.245 ± 0.865
3.529AlaAsn: 3.529 ± 0.401
2.96AlaPro: 2.96 ± 0.435
3.245AlaGln: 3.245 ± 0.403
4.724AlaArg: 4.724 ± 0.482
5.18AlaSer: 5.18 ± 0.528
5.635AlaThr: 5.635 ± 0.471
5.977AlaVal: 5.977 ± 0.671
1.708AlaTrp: 1.708 ± 0.355
2.96AlaTyr: 2.96 ± 0.452
0.0AlaXaa: 0.0 ± 0.0
Cys
0.342CysAla: 0.342 ± 0.179
0.057CysCys: 0.057 ± 0.055
0.455CysAsp: 0.455 ± 0.156
0.342CysGlu: 0.342 ± 0.123
0.285CysPhe: 0.285 ± 0.126
0.512CysGly: 0.512 ± 0.183
0.114CysHis: 0.114 ± 0.09
0.228CysIle: 0.228 ± 0.137
0.285CysLys: 0.285 ± 0.143
0.569CysLeu: 0.569 ± 0.198
0.0CysMet: 0.0 ± 0.0
0.171CysAsn: 0.171 ± 0.089
0.171CysPro: 0.171 ± 0.094
0.228CysGln: 0.228 ± 0.106
0.228CysArg: 0.228 ± 0.108
0.285CysSer: 0.285 ± 0.133
0.171CysThr: 0.171 ± 0.105
0.342CysVal: 0.342 ± 0.151
0.057CysTrp: 0.057 ± 0.053
0.114CysTyr: 0.114 ± 0.073
0.0CysXaa: 0.0 ± 0.0
Asp
6.489AspAla: 6.489 ± 0.656
0.171AspCys: 0.171 ± 0.104
3.529AspAsp: 3.529 ± 0.603
4.44AspGlu: 4.44 ± 0.566
2.618AspPhe: 2.618 ± 0.488
5.578AspGly: 5.578 ± 0.65
0.512AspHis: 0.512 ± 0.165
5.18AspIle: 5.18 ± 0.467
3.131AspLys: 3.131 ± 0.513
4.668AspLeu: 4.668 ± 0.581
1.878AspMet: 1.878 ± 0.229
2.903AspAsn: 2.903 ± 0.43
2.732AspPro: 2.732 ± 0.476
2.277AspGln: 2.277 ± 0.346
2.732AspArg: 2.732 ± 0.449
2.505AspSer: 2.505 ± 0.31
2.96AspThr: 2.96 ± 0.489
3.358AspVal: 3.358 ± 0.455
1.138AspTrp: 1.138 ± 0.231
1.537AspTyr: 1.537 ± 0.273
0.0AspXaa: 0.0 ± 0.0
Glu
5.066GluAla: 5.066 ± 0.587
0.455GluCys: 0.455 ± 0.177
3.871GluAsp: 3.871 ± 0.59
5.294GluGlu: 5.294 ± 0.548
2.789GluPhe: 2.789 ± 0.407
4.212GluGly: 4.212 ± 0.421
1.935GluHis: 1.935 ± 0.326
4.098GluIle: 4.098 ± 0.458
4.155GluLys: 4.155 ± 0.53
5.18GluLeu: 5.18 ± 0.541
2.22GluMet: 2.22 ± 0.317
2.732GluAsn: 2.732 ± 0.362
2.618GluPro: 2.618 ± 0.488
2.846GluGln: 2.846 ± 0.395
4.041GluArg: 4.041 ± 0.51
2.618GluSer: 2.618 ± 0.375
4.041GluThr: 4.041 ± 0.629
5.294GluVal: 5.294 ± 0.797
1.765GluTrp: 1.765 ± 0.391
1.651GluTyr: 1.651 ± 0.315
0.0GluXaa: 0.0 ± 0.0
Phe
3.074PheAla: 3.074 ± 0.471
0.398PheCys: 0.398 ± 0.154
2.789PheAsp: 2.789 ± 0.332
1.878PheGlu: 1.878 ± 0.351
1.423PhePhe: 1.423 ± 0.274
2.732PheGly: 2.732 ± 0.363
0.569PheHis: 0.569 ± 0.2
2.334PheIle: 2.334 ± 0.316
1.992PheLys: 1.992 ± 0.309
2.96PheLeu: 2.96 ± 0.327
0.74PheMet: 0.74 ± 0.179
2.675PheAsn: 2.675 ± 0.309
1.252PhePro: 1.252 ± 0.239
1.082PheGln: 1.082 ± 0.3
2.391PheArg: 2.391 ± 0.418
2.163PheSer: 2.163 ± 0.39
2.732PheThr: 2.732 ± 0.366
2.334PheVal: 2.334 ± 0.406
0.797PheTrp: 0.797 ± 0.222
0.968PheTyr: 0.968 ± 0.265
0.0PheXaa: 0.0 ± 0.0
Gly
6.888GlyAla: 6.888 ± 0.706
0.398GlyCys: 0.398 ± 0.169
4.44GlyAsp: 4.44 ± 0.551
4.44GlyGlu: 4.44 ± 0.428
3.074GlyPhe: 3.074 ± 0.483
6.603GlyGly: 6.603 ± 0.804
1.309GlyHis: 1.309 ± 0.344
4.497GlyIle: 4.497 ± 0.703
4.554GlyLys: 4.554 ± 0.44
6.546GlyLeu: 6.546 ± 1.223
2.277GlyMet: 2.277 ± 0.683
3.529GlyAsn: 3.529 ± 0.547
1.765GlyPro: 1.765 ± 0.391
1.992GlyGln: 1.992 ± 0.333
2.846GlyArg: 2.846 ± 0.393
4.724GlySer: 4.724 ± 0.594
6.944GlyThr: 6.944 ± 0.913
5.294GlyVal: 5.294 ± 0.825
1.594GlyTrp: 1.594 ± 0.335
2.846GlyTyr: 2.846 ± 0.332
0.0GlyXaa: 0.0 ± 0.0
His
1.935HisAla: 1.935 ± 0.41
0.057HisCys: 0.057 ± 0.045
0.968HisAsp: 0.968 ± 0.241
0.968HisGlu: 0.968 ± 0.184
0.285HisPhe: 0.285 ± 0.13
1.423HisGly: 1.423 ± 0.334
0.74HisHis: 0.74 ± 0.168
1.309HisIle: 1.309 ± 0.323
0.968HisLys: 0.968 ± 0.199
1.195HisLeu: 1.195 ± 0.233
0.569HisMet: 0.569 ± 0.183
1.138HisAsn: 1.138 ± 0.282
0.968HisPro: 0.968 ± 0.224
0.854HisGln: 0.854 ± 0.203
0.797HisArg: 0.797 ± 0.23
1.195HisSer: 1.195 ± 0.33
1.195HisThr: 1.195 ± 0.31
1.309HisVal: 1.309 ± 0.281
0.171HisTrp: 0.171 ± 0.094
0.911HisTyr: 0.911 ± 0.212
0.0HisXaa: 0.0 ± 0.0
Ile
5.863IleAla: 5.863 ± 0.953
0.455IleCys: 0.455 ± 0.177
4.724IleAsp: 4.724 ± 0.48
4.041IleGlu: 4.041 ± 0.478
1.537IlePhe: 1.537 ± 0.315
3.643IleGly: 3.643 ± 0.599
1.48IleHis: 1.48 ± 0.255
3.131IleIle: 3.131 ± 0.482
4.155IleLys: 4.155 ± 0.503
5.351IleLeu: 5.351 ± 0.65
1.765IleMet: 1.765 ± 0.341
3.131IleAsn: 3.131 ± 0.447
2.561IlePro: 2.561 ± 0.47
1.935IleGln: 1.935 ± 0.295
2.049IleArg: 2.049 ± 0.314
3.928IleSer: 3.928 ± 0.48
3.358IleThr: 3.358 ± 0.429
5.123IleVal: 5.123 ± 0.469
0.854IleTrp: 0.854 ± 0.242
1.708IleTyr: 1.708 ± 0.277
0.0IleXaa: 0.0 ± 0.0
Lys
5.977LysAla: 5.977 ± 0.631
0.228LysCys: 0.228 ± 0.096
2.846LysAsp: 2.846 ± 0.65
5.749LysGlu: 5.749 ± 0.647
2.789LysPhe: 2.789 ± 0.435
4.155LysGly: 4.155 ± 0.518
1.537LysHis: 1.537 ± 0.441
3.415LysIle: 3.415 ± 0.528
5.237LysLys: 5.237 ± 0.875
5.123LysLeu: 5.123 ± 0.584
2.22LysMet: 2.22 ± 0.396
2.391LysAsn: 2.391 ± 0.369
2.903LysPro: 2.903 ± 0.383
2.789LysGln: 2.789 ± 0.438
4.098LysArg: 4.098 ± 0.47
3.188LysSer: 3.188 ± 0.43
2.732LysThr: 2.732 ± 0.42
3.358LysVal: 3.358 ± 0.423
1.082LysTrp: 1.082 ± 0.252
1.878LysTyr: 1.878 ± 0.312
0.0LysXaa: 0.0 ± 0.0
Leu
7.172LeuAla: 7.172 ± 0.776
0.626LeuCys: 0.626 ± 0.192
5.066LeuAsp: 5.066 ± 0.588
5.635LeuGlu: 5.635 ± 0.538
2.903LeuPhe: 2.903 ± 0.568
6.261LeuGly: 6.261 ± 1.273
1.195LeuHis: 1.195 ± 0.241
5.92LeuIle: 5.92 ± 0.737
5.123LeuLys: 5.123 ± 0.741
3.871LeuLeu: 3.871 ± 0.667
2.106LeuMet: 2.106 ± 0.425
3.472LeuAsn: 3.472 ± 0.459
2.96LeuPro: 2.96 ± 0.402
2.505LeuGln: 2.505 ± 0.3
4.041LeuArg: 4.041 ± 0.528
4.838LeuSer: 4.838 ± 0.629
5.066LeuThr: 5.066 ± 0.473
5.635LeuVal: 5.635 ± 0.702
0.854LeuTrp: 0.854 ± 0.235
2.789LeuTyr: 2.789 ± 0.435
0.0LeuXaa: 0.0 ± 0.0
Met
3.415MetAla: 3.415 ± 0.348
0.114MetCys: 0.114 ± 0.097
1.992MetAsp: 1.992 ± 0.325
1.708MetGlu: 1.708 ± 0.347
0.911MetPhe: 0.911 ± 0.204
2.049MetGly: 2.049 ± 0.738
0.512MetHis: 0.512 ± 0.141
1.195MetIle: 1.195 ± 0.356
1.651MetLys: 1.651 ± 0.33
2.789MetLeu: 2.789 ± 0.431
0.74MetMet: 0.74 ± 0.461
0.797MetAsn: 0.797 ± 0.202
1.309MetPro: 1.309 ± 0.197
0.968MetGln: 0.968 ± 0.427
1.366MetArg: 1.366 ± 0.291
2.732MetSer: 2.732 ± 0.366
1.992MetThr: 1.992 ± 0.317
1.878MetVal: 1.878 ± 0.298
0.171MetTrp: 0.171 ± 0.084
0.911MetTyr: 0.911 ± 0.189
0.0MetXaa: 0.0 ± 0.0
Asn
4.44AsnAla: 4.44 ± 0.509
0.285AsnCys: 0.285 ± 0.121
3.358AsnAsp: 3.358 ± 0.474
2.448AsnGlu: 2.448 ± 0.406
2.049AsnPhe: 2.049 ± 0.295
4.269AsnGly: 4.269 ± 0.63
0.626AsnHis: 0.626 ± 0.207
2.561AsnIle: 2.561 ± 0.45
2.561AsnLys: 2.561 ± 0.471
3.643AsnLeu: 3.643 ± 0.363
1.366AsnMet: 1.366 ± 0.305
2.561AsnAsn: 2.561 ± 0.422
2.505AsnPro: 2.505 ± 0.337
1.651AsnGln: 1.651 ± 0.325
2.277AsnArg: 2.277 ± 0.441
2.96AsnSer: 2.96 ± 0.472
2.561AsnThr: 2.561 ± 0.382
2.675AsnVal: 2.675 ± 0.296
0.797AsnTrp: 0.797 ± 0.195
1.651AsnTyr: 1.651 ± 0.273
0.0AsnXaa: 0.0 ± 0.0
Pro
3.7ProAla: 3.7 ± 0.549
0.342ProCys: 0.342 ± 0.147
2.789ProAsp: 2.789 ± 0.379
3.472ProGlu: 3.472 ± 0.467
0.683ProPhe: 0.683 ± 0.197
2.846ProGly: 2.846 ± 0.387
0.854ProHis: 0.854 ± 0.217
2.277ProIle: 2.277 ± 0.351
2.334ProLys: 2.334 ± 0.352
2.448ProLeu: 2.448 ± 0.36
1.195ProMet: 1.195 ± 0.244
1.821ProAsn: 1.821 ± 0.428
2.106ProPro: 2.106 ± 0.397
1.025ProGln: 1.025 ± 0.205
2.391ProArg: 2.391 ± 0.393
2.505ProSer: 2.505 ± 0.314
2.903ProThr: 2.903 ± 0.423
3.472ProVal: 3.472 ± 0.432
0.285ProTrp: 0.285 ± 0.127
1.309ProTyr: 1.309 ± 0.3
0.0ProXaa: 0.0 ± 0.0
Gln
2.846GlnAla: 2.846 ± 0.437
0.114GlnCys: 0.114 ± 0.084
1.366GlnAsp: 1.366 ± 0.288
2.505GlnGlu: 2.505 ± 0.425
1.537GlnPhe: 1.537 ± 0.353
2.846GlnGly: 2.846 ± 0.396
0.74GlnHis: 0.74 ± 0.229
1.992GlnIle: 1.992 ± 0.308
3.017GlnLys: 3.017 ± 0.449
3.358GlnLeu: 3.358 ± 0.477
1.082GlnMet: 1.082 ± 0.216
1.821GlnAsn: 1.821 ± 0.302
1.195GlnPro: 1.195 ± 0.285
2.163GlnGln: 2.163 ± 0.285
1.992GlnArg: 1.992 ± 0.314
1.025GlnSer: 1.025 ± 0.217
1.935GlnThr: 1.935 ± 0.33
2.675GlnVal: 2.675 ± 0.428
0.626GlnTrp: 0.626 ± 0.185
1.138GlnTyr: 1.138 ± 0.208
0.0GlnXaa: 0.0 ± 0.0
Arg
3.643ArgAla: 3.643 ± 0.548
0.171ArgCys: 0.171 ± 0.094
3.131ArgAsp: 3.131 ± 0.469
3.358ArgGlu: 3.358 ± 0.421
1.252ArgPhe: 1.252 ± 0.296
2.846ArgGly: 2.846 ± 0.38
1.025ArgHis: 1.025 ± 0.216
3.188ArgIle: 3.188 ± 0.374
4.155ArgLys: 4.155 ± 0.527
4.497ArgLeu: 4.497 ± 0.464
0.797ArgMet: 0.797 ± 0.21
2.675ArgAsn: 2.675 ± 0.333
1.878ArgPro: 1.878 ± 0.347
2.334ArgGln: 2.334 ± 0.413
3.529ArgArg: 3.529 ± 0.629
2.96ArgSer: 2.96 ± 0.36
3.131ArgThr: 3.131 ± 0.421
4.098ArgVal: 4.098 ± 0.427
0.626ArgTrp: 0.626 ± 0.21
2.391ArgTyr: 2.391 ± 0.402
0.0ArgXaa: 0.0 ± 0.0
Ser
3.928SerAla: 3.928 ± 0.421
0.114SerCys: 0.114 ± 0.085
3.472SerAsp: 3.472 ± 0.374
3.7SerGlu: 3.7 ± 0.497
2.618SerPhe: 2.618 ± 0.365
4.952SerGly: 4.952 ± 0.496
0.968SerHis: 0.968 ± 0.211
3.415SerIle: 3.415 ± 0.433
4.041SerLys: 4.041 ± 0.509
5.066SerLeu: 5.066 ± 0.582
1.878SerMet: 1.878 ± 0.293
2.505SerAsn: 2.505 ± 0.352
2.903SerPro: 2.903 ± 0.432
1.878SerGln: 1.878 ± 0.361
2.618SerArg: 2.618 ± 0.288
4.155SerSer: 4.155 ± 0.717
3.415SerThr: 3.415 ± 0.402
3.472SerVal: 3.472 ± 0.467
1.195SerTrp: 1.195 ± 0.209
1.48SerTyr: 1.48 ± 0.299
0.0SerXaa: 0.0 ± 0.0
Thr
4.895ThrAla: 4.895 ± 0.397
0.114ThrCys: 0.114 ± 0.084
4.041ThrAsp: 4.041 ± 0.624
4.041ThrGlu: 4.041 ± 0.481
2.675ThrPhe: 2.675 ± 0.274
5.692ThrGly: 5.692 ± 0.625
0.854ThrHis: 0.854 ± 0.247
3.415ThrIle: 3.415 ± 0.41
2.789ThrLys: 2.789 ± 0.399
5.066ThrLeu: 5.066 ± 0.532
1.651ThrMet: 1.651 ± 0.283
3.188ThrAsn: 3.188 ± 0.464
3.472ThrPro: 3.472 ± 0.417
2.106ThrGln: 2.106 ± 0.374
2.561ThrArg: 2.561 ± 0.342
3.188ThrSer: 3.188 ± 0.424
4.383ThrThr: 4.383 ± 0.57
4.838ThrVal: 4.838 ± 0.692
0.911ThrTrp: 0.911 ± 0.238
2.448ThrTyr: 2.448 ± 0.346
0.0ThrXaa: 0.0 ± 0.0
Val
6.091ValAla: 6.091 ± 0.579
0.285ValCys: 0.285 ± 0.161
3.188ValAsp: 3.188 ± 0.364
3.928ValGlu: 3.928 ± 0.473
2.049ValPhe: 2.049 ± 0.353
4.44ValGly: 4.44 ± 0.543
1.252ValHis: 1.252 ± 0.277
4.895ValIle: 4.895 ± 0.415
5.066ValLys: 5.066 ± 0.512
5.977ValLeu: 5.977 ± 0.729
2.22ValMet: 2.22 ± 0.27
3.757ValAsn: 3.757 ± 0.41
3.131ValPro: 3.131 ± 0.36
2.163ValGln: 2.163 ± 0.366
4.611ValArg: 4.611 ± 0.569
4.098ValSer: 4.098 ± 0.491
3.529ValThr: 3.529 ± 0.481
5.18ValVal: 5.18 ± 0.557
0.968ValTrp: 0.968 ± 0.165
2.505ValTyr: 2.505 ± 0.449
0.0ValXaa: 0.0 ± 0.0
Trp
1.423TrpAla: 1.423 ± 0.34
0.0TrpCys: 0.0 ± 0.0
0.968TrpAsp: 0.968 ± 0.281
1.252TrpGlu: 1.252 ± 0.294
0.911TrpPhe: 0.911 ± 0.222
1.309TrpGly: 1.309 ± 0.241
0.342TrpHis: 0.342 ± 0.15
0.683TrpIle: 0.683 ± 0.201
0.626TrpLys: 0.626 ± 0.182
0.968TrpLeu: 0.968 ± 0.253
0.512TrpMet: 0.512 ± 0.141
1.082TrpAsn: 1.082 ± 0.276
0.228TrpPro: 0.228 ± 0.127
0.626TrpGln: 0.626 ± 0.199
0.74TrpArg: 0.74 ± 0.317
0.854TrpSer: 0.854 ± 0.212
1.48TrpThr: 1.48 ± 0.287
1.195TrpVal: 1.195 ± 0.306
0.228TrpTrp: 0.228 ± 0.117
0.683TrpTyr: 0.683 ± 0.2
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.017TyrAla: 3.017 ± 0.481
0.171TyrCys: 0.171 ± 0.099
2.561TyrAsp: 2.561 ± 0.56
1.878TyrGlu: 1.878 ± 0.318
1.651TyrPhe: 1.651 ± 0.319
2.334TyrGly: 2.334 ± 0.323
0.569TyrHis: 0.569 ± 0.18
1.992TyrIle: 1.992 ± 0.325
2.22TyrLys: 2.22 ± 0.321
2.391TyrLeu: 2.391 ± 0.381
0.683TyrMet: 0.683 ± 0.227
1.366TyrAsn: 1.366 ± 0.209
1.138TyrPro: 1.138 ± 0.224
1.309TyrGln: 1.309 ± 0.259
1.651TyrArg: 1.651 ± 0.277
2.675TyrSer: 2.675 ± 0.349
2.163TyrThr: 2.163 ± 0.417
1.821TyrVal: 1.821 ± 0.308
0.285TyrTrp: 0.285 ± 0.126
1.366TyrTyr: 1.366 ± 0.314
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 85 proteins (17569 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski