Actinomyces sp. 2119

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinomyces; unclassified Actinomyces

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2334 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A418UR61|A0A418UR61_9ACTO Copper-binding protein OS=Actinomyces sp. 2119 OX=2321393 GN=D4740_10715 PE=4 SV=1
MM1 pKa = 7.29LRR3 pKa = 11.84IGNRR7 pKa = 11.84VTDD10 pKa = 3.75TFTVGNRR17 pKa = 11.84TSTALGLTNTADD29 pKa = 3.84YY30 pKa = 11.06KK31 pKa = 10.87PGTLAVTKK39 pKa = 9.79TVTGLEE45 pKa = 3.92AAAPRR50 pKa = 11.84TYY52 pKa = 10.71SYY54 pKa = 10.53TYY56 pKa = 9.36TCGEE60 pKa = 4.06VTGTISNVPGDD71 pKa = 4.18GTPVEE76 pKa = 4.61AGATIPAGTEE86 pKa = 3.87CTVTEE91 pKa = 4.57DD92 pKa = 2.93VDD94 pKa = 3.6AAAMDD99 pKa = 4.97GYY101 pKa = 11.19DD102 pKa = 4.27LAAPDD107 pKa = 4.44PVTVTVVPSEE117 pKa = 4.15EE118 pKa = 4.25AVTEE122 pKa = 4.02AAFTNSYY129 pKa = 8.72TRR131 pKa = 11.84HH132 pKa = 5.79TGTFSVAKK140 pKa = 9.7QVGPEE145 pKa = 4.13DD146 pKa = 3.82VPFLADD152 pKa = 3.68TFDD155 pKa = 3.23VDD157 pKa = 4.06YY158 pKa = 10.44TCTQPEE164 pKa = 4.37GTEE167 pKa = 3.99VSGTLQVTGGAPAVNGPTLPVGTTCSVSEE196 pKa = 4.39PEE198 pKa = 4.18EE199 pKa = 3.91TTQRR203 pKa = 11.84DD204 pKa = 4.2GYY206 pKa = 10.93NVSTLIRR213 pKa = 11.84VDD215 pKa = 3.26GTEE218 pKa = 3.96GSRR221 pKa = 11.84LTITRR226 pKa = 11.84DD227 pKa = 3.16TTSEE231 pKa = 4.05VSVVNAYY238 pKa = 7.48TALTGGFQISKK249 pKa = 10.1EE250 pKa = 4.14VTGDD254 pKa = 3.38GAGLADD260 pKa = 3.48SQTYY264 pKa = 9.16VFTYY268 pKa = 9.48TCTGVNGTTTTDD280 pKa = 3.16TVEE283 pKa = 4.21LVSGATTAVTDD294 pKa = 4.02VPVGSCTVNEE304 pKa = 4.29ADD306 pKa = 4.75ASVDD310 pKa = 3.6GADD313 pKa = 4.65LTTTWSVDD321 pKa = 3.09GEE323 pKa = 4.37EE324 pKa = 4.37TDD326 pKa = 4.36GEE328 pKa = 4.8VTFDD332 pKa = 3.55VADD335 pKa = 4.01GATAVVQATNDD346 pKa = 3.66YY347 pKa = 8.36TVHH350 pKa = 6.92RR351 pKa = 11.84GGFSVAKK358 pKa = 9.11EE359 pKa = 3.86VTGAEE364 pKa = 4.14EE365 pKa = 4.43ADD367 pKa = 3.76LSAKK371 pKa = 10.11EE372 pKa = 4.04FGVTYY377 pKa = 9.53TCTDD381 pKa = 2.72GSTGVLAVGADD392 pKa = 3.94GQSVSGPQVPVGTEE406 pKa = 3.94CTVSEE411 pKa = 4.5DD412 pKa = 3.48LASASLAGYY421 pKa = 7.48TLTAPEE427 pKa = 4.32DD428 pKa = 3.44QAVTVEE434 pKa = 4.07ARR436 pKa = 11.84GQEE439 pKa = 4.15TALTLSNAYY448 pKa = 8.27TRR450 pKa = 11.84QTGSFAVSKK459 pKa = 9.71TVDD462 pKa = 2.97GDD464 pKa = 3.72GAARR468 pKa = 11.84APQSFSFAYY477 pKa = 8.98TCTGVDD483 pKa = 3.37GTTTTGTVPAADD495 pKa = 4.01ADD497 pKa = 4.29SAVVTDD503 pKa = 4.09VPVGRR508 pKa = 11.84CTISEE513 pKa = 3.9EE514 pKa = 4.14DD515 pKa = 3.61ASVEE519 pKa = 4.11GTDD522 pKa = 5.31LLTQTVVDD530 pKa = 4.43GDD532 pKa = 3.95PVITEE537 pKa = 4.12EE538 pKa = 4.95ASLTVTDD545 pKa = 3.97GGMVAVVVTNTYY557 pKa = 8.24TLHH560 pKa = 6.7RR561 pKa = 11.84GSFSVTKK568 pKa = 10.36VVEE571 pKa = 3.99GLEE574 pKa = 4.55DD575 pKa = 4.07DD576 pKa = 5.2SEE578 pKa = 4.66DD579 pKa = 4.79AGDD582 pKa = 4.78PDD584 pKa = 3.86AQAAPRR590 pKa = 11.84DD591 pKa = 3.71YY592 pKa = 11.08LFTYY596 pKa = 9.39TCTLPEE602 pKa = 4.47DD603 pKa = 3.78AADD606 pKa = 4.06AEE608 pKa = 4.71GGADD612 pKa = 3.3GGVVSDD618 pKa = 4.32QVTVPAGMTVTSPALPVGTEE638 pKa = 4.11CTVSEE643 pKa = 4.43DD644 pKa = 3.53AEE646 pKa = 4.09AAAVDD651 pKa = 5.34GYY653 pKa = 11.52ALEE656 pKa = 4.4PAEE659 pKa = 4.46DD660 pKa = 3.55QAVTVSTQDD669 pKa = 3.1TVEE672 pKa = 4.42EE673 pKa = 4.17MTFTNTYY680 pKa = 6.96TRR682 pKa = 11.84EE683 pKa = 4.1VEE685 pKa = 4.2PTPLPSEE692 pKa = 4.39SPAPQPSGEE701 pKa = 4.22PSTGPSAVVTSGPATPSSEE720 pKa = 4.39VPASASPSPGGSSVTGSLARR740 pKa = 11.84TGAAILLPAVAALAAIGGGSLLLRR764 pKa = 11.84RR765 pKa = 11.84RR766 pKa = 11.84RR767 pKa = 11.84QQ768 pKa = 3.05

Molecular weight: 77.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A418UQ06|A0A418UQ06_9ACTO TatD family deoxyribonuclease OS=Actinomyces sp. 2119 OX=2321393 GN=D4740_12250 PE=4 SV=1
MM1 pKa = 7.32LLVEE5 pKa = 4.58RR6 pKa = 11.84VLVSLLPTLLKK17 pKa = 10.53PLGHH21 pKa = 6.63RR22 pKa = 11.84PITRR26 pKa = 11.84GATITVTVRR35 pKa = 11.84ATAATLGRR43 pKa = 11.84AVPATTTAGATRR55 pKa = 11.84AVTTTTGTVSTVSRR69 pKa = 11.84VAGRR73 pKa = 11.84GLTVSPVSPVAARR86 pKa = 11.84RR87 pKa = 11.84GIRR90 pKa = 11.84ISTLGVGAVTGRR102 pKa = 11.84GLTVSTLSAVTASRR116 pKa = 11.84GLTIGALTTAVTRR129 pKa = 11.84RR130 pKa = 11.84RR131 pKa = 11.84LRR133 pKa = 11.84TITISPVAPRR143 pKa = 11.84RR144 pKa = 11.84GTRR147 pKa = 11.84LSRR150 pKa = 11.84VTISTVAGRR159 pKa = 11.84GARR162 pKa = 11.84LSGTVSVSLVATPRR176 pKa = 11.84TRR178 pKa = 11.84LSRR181 pKa = 3.68

Molecular weight: 18.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2334

0

2334

835853

32

4575

358.1

38.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.126 ± 0.06

0.809 ± 0.016

5.764 ± 0.039

5.709 ± 0.04

2.316 ± 0.029

9.352 ± 0.039

2.052 ± 0.022

2.88 ± 0.035

1.327 ± 0.021

10.323 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.855 ± 0.022

1.548 ± 0.022

5.767 ± 0.05

3.448 ± 0.023

7.807 ± 0.053

6.373 ± 0.038

6.378 ± 0.05

9.727 ± 0.048

1.463 ± 0.02

1.977 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski