Cow vetch latent virus alphasatellite
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4T2E5|A0A2H4T2E5_9VIRU Replication initiator protein OS=Cow vetch latent virus alphasatellite OX=2056781 GN=Rep PE=4 SV=1
MM1 pKa = 7.53 PSIRR5 pKa = 11.84 GNMWAFTLNFIGPIPNLNWGPEE27 pKa = 3.77 VQYY30 pKa = 11.4 AKK32 pKa = 9.58 WQHH35 pKa = 5.06 EE36 pKa = 4.73 KK37 pKa = 10.29 KK38 pKa = 10.68 AHH40 pKa = 6.13 DD41 pKa = 4.0 HH42 pKa = 5.64 LQGFIQLKK50 pKa = 10.1 EE51 pKa = 3.92 RR52 pKa = 11.84 MTLKK56 pKa = 10.42 KK57 pKa = 9.92 MKK59 pKa = 9.99 ILIPGAHH66 pKa = 6.91 LEE68 pKa = 4.26 LCKK71 pKa = 10.56 GTVEE75 pKa = 5.88 DD76 pKa = 4.15 NLNYY80 pKa = 9.86 CGKK83 pKa = 10.1 VEE85 pKa = 4.1 TRR87 pKa = 11.84 VTGPWEE93 pKa = 3.58 YY94 pKa = 11.73 GEE96 pKa = 3.92 ILKK99 pKa = 10.73 RR100 pKa = 11.84 GSNKK104 pKa = 9.59 RR105 pKa = 11.84 KK106 pKa = 10.15 VMDD109 pKa = 4.66 RR110 pKa = 11.84 YY111 pKa = 10.82 EE112 pKa = 4.89 DD113 pKa = 4.56 DD114 pKa = 4.16 PDD116 pKa = 3.79 AMKK119 pKa = 10.34 MEE121 pKa = 5.22 DD122 pKa = 3.44 PDD124 pKa = 5.02 LYY126 pKa = 10.97 LRR128 pKa = 11.84 CNARR132 pKa = 11.84 RR133 pKa = 11.84 LEE135 pKa = 3.96 KK136 pKa = 10.38 EE137 pKa = 4.21 YY138 pKa = 10.57 MSSCGPATLSGWQIEE153 pKa = 4.15 LHH155 pKa = 5.74 EE156 pKa = 4.87 ALMAEE161 pKa = 4.44 PDD163 pKa = 3.84 DD164 pKa = 3.95 RR165 pKa = 11.84 TIIWVYY171 pKa = 10.71 GPVGGEE177 pKa = 4.17 GKK179 pKa = 8.45 STFAKK184 pKa = 10.37 EE185 pKa = 3.95 LIKK188 pKa = 10.68 RR189 pKa = 11.84 NYY191 pKa = 9.23 FYY193 pKa = 11.4 SPGGRR198 pKa = 11.84 TEE200 pKa = 4.41 NILYY204 pKa = 9.67 LYY206 pKa = 11.35 SMDD209 pKa = 3.94 PEE211 pKa = 4.46 RR212 pKa = 11.84 NCVFDD217 pKa = 5.74 FPRR220 pKa = 11.84 CNKK223 pKa = 10.28 DD224 pKa = 3.44 FINYY228 pKa = 9.49 SVIEE232 pKa = 4.19 MIKK235 pKa = 10.5 NGVFSSTKK243 pKa = 9.63 YY244 pKa = 9.45 RR245 pKa = 11.84 PVDD248 pKa = 2.99 IRR250 pKa = 11.84 VIKK253 pKa = 9.2 PRR255 pKa = 11.84 HH256 pKa = 4.44 VVIMCNEE263 pKa = 3.99 LPDD266 pKa = 3.58 VTKK269 pKa = 10.66 ISEE272 pKa = 3.96 DD273 pKa = 3.59 RR274 pKa = 11.84 IKK276 pKa = 10.47 IIYY279 pKa = 8.96 CC280 pKa = 3.79
Molecular weight: 32.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.019
IPC2_protein 7.366
IPC_protein 7.278
Toseland 6.927
ProMoST 7.878
Dawson 7.966
Bjellqvist 8.302
Wikipedia 7.878
Rodwell 7.98
Grimsley 6.956
Solomon 8.039
Lehninger 8.053
Nozaki 8.521
DTASelect 8.068
Thurlkill 8.126
EMBOSS 8.185
Sillero 8.448
Patrickios 4.228
IPC_peptide 8.039
IPC2_peptide 7.366
IPC2.peptide.svr19 7.461
Protein with the highest isoelectric point:
>tr|A0A2H4T2E5|A0A2H4T2E5_9VIRU Replication initiator protein OS=Cow vetch latent virus alphasatellite OX=2056781 GN=Rep PE=4 SV=1
MM1 pKa = 7.53 PSIRR5 pKa = 11.84 GNMWAFTLNFIGPIPNLNWGPEE27 pKa = 3.77 VQYY30 pKa = 11.4 AKK32 pKa = 9.58 WQHH35 pKa = 5.06 EE36 pKa = 4.73 KK37 pKa = 10.29 KK38 pKa = 10.68 AHH40 pKa = 6.13 DD41 pKa = 4.0 HH42 pKa = 5.64 LQGFIQLKK50 pKa = 10.1 EE51 pKa = 3.92 RR52 pKa = 11.84 MTLKK56 pKa = 10.42 KK57 pKa = 9.92 MKK59 pKa = 9.99 ILIPGAHH66 pKa = 6.91 LEE68 pKa = 4.26 LCKK71 pKa = 10.56 GTVEE75 pKa = 5.88 DD76 pKa = 4.15 NLNYY80 pKa = 9.86 CGKK83 pKa = 10.1 VEE85 pKa = 4.1 TRR87 pKa = 11.84 VTGPWEE93 pKa = 3.58 YY94 pKa = 11.73 GEE96 pKa = 3.92 ILKK99 pKa = 10.73 RR100 pKa = 11.84 GSNKK104 pKa = 9.59 RR105 pKa = 11.84 KK106 pKa = 10.15 VMDD109 pKa = 4.66 RR110 pKa = 11.84 YY111 pKa = 10.82 EE112 pKa = 4.89 DD113 pKa = 4.56 DD114 pKa = 4.16 PDD116 pKa = 3.79 AMKK119 pKa = 10.34 MEE121 pKa = 5.22 DD122 pKa = 3.44 PDD124 pKa = 5.02 LYY126 pKa = 10.97 LRR128 pKa = 11.84 CNARR132 pKa = 11.84 RR133 pKa = 11.84 LEE135 pKa = 3.96 KK136 pKa = 10.38 EE137 pKa = 4.21 YY138 pKa = 10.57 MSSCGPATLSGWQIEE153 pKa = 4.15 LHH155 pKa = 5.74 EE156 pKa = 4.87 ALMAEE161 pKa = 4.44 PDD163 pKa = 3.84 DD164 pKa = 3.95 RR165 pKa = 11.84 TIIWVYY171 pKa = 10.71 GPVGGEE177 pKa = 4.17 GKK179 pKa = 8.45 STFAKK184 pKa = 10.37 EE185 pKa = 3.95 LIKK188 pKa = 10.68 RR189 pKa = 11.84 NYY191 pKa = 9.23 FYY193 pKa = 11.4 SPGGRR198 pKa = 11.84 TEE200 pKa = 4.41 NILYY204 pKa = 9.67 LYY206 pKa = 11.35 SMDD209 pKa = 3.94 PEE211 pKa = 4.46 RR212 pKa = 11.84 NCVFDD217 pKa = 5.74 FPRR220 pKa = 11.84 CNKK223 pKa = 10.28 DD224 pKa = 3.44 FINYY228 pKa = 9.49 SVIEE232 pKa = 4.19 MIKK235 pKa = 10.5 NGVFSSTKK243 pKa = 9.63 YY244 pKa = 9.45 RR245 pKa = 11.84 PVDD248 pKa = 2.99 IRR250 pKa = 11.84 VIKK253 pKa = 9.2 PRR255 pKa = 11.84 HH256 pKa = 4.44 VVIMCNEE263 pKa = 3.99 LPDD266 pKa = 3.58 VTKK269 pKa = 10.66 ISEE272 pKa = 3.96 DD273 pKa = 3.59 RR274 pKa = 11.84 IKK276 pKa = 10.47 IIYY279 pKa = 8.96 CC280 pKa = 3.79
Molecular weight: 32.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.019
IPC2_protein 7.366
IPC_protein 7.278
Toseland 6.927
ProMoST 7.878
Dawson 7.966
Bjellqvist 8.302
Wikipedia 7.878
Rodwell 7.98
Grimsley 6.956
Solomon 8.039
Lehninger 8.053
Nozaki 8.521
DTASelect 8.068
Thurlkill 8.126
EMBOSS 8.185
Sillero 8.448
Patrickios 4.228
IPC_peptide 8.039
IPC2_peptide 7.366
IPC2.peptide.svr19 7.461
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
280
280
280
280.0
32.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.571 ± 0.0
2.857 ± 0.0
5.714 ± 0.0
7.857 ± 0.0
3.214 ± 0.0
6.786 ± 0.0
2.143 ± 0.0
7.857 ± 0.0
8.214 ± 0.0
7.143 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.286 ± 0.0
5.357 ± 0.0
6.071 ± 0.0
1.786 ± 0.0
6.429 ± 0.0
4.286 ± 0.0
3.929 ± 0.0
5.357 ± 0.0
2.143 ± 0.0
5.0 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here