Amino acid dipepetide frequency for Vulcanisaeta distributa (strain DSM 14429 / JCM 11212 / NBRC 100878 / IC-017)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.002AlaAla: 5.002 ± 0.111
0.475AlaCys: 0.475 ± 0.028
2.424AlaAsp: 2.424 ± 0.061
3.53AlaGlu: 3.53 ± 0.081
2.699AlaPhe: 2.699 ± 0.075
4.451AlaGly: 4.451 ± 0.108
1.023AlaHis: 1.023 ± 0.039
6.589AlaIle: 6.589 ± 0.102
3.241AlaLys: 3.241 ± 0.083
9.611AlaLeu: 9.611 ± 0.142
2.337AlaMet: 2.337 ± 0.054
2.231AlaAsn: 2.231 ± 0.059
2.297AlaPro: 2.297 ± 0.062
1.647AlaGln: 1.647 ± 0.051
4.696AlaArg: 4.696 ± 0.104
4.151AlaSer: 4.151 ± 0.087
3.017AlaThr: 3.017 ± 0.079
6.805AlaVal: 6.805 ± 0.112
1.084AlaTrp: 1.084 ± 0.039
3.306AlaTyr: 3.306 ± 0.079
0.0AlaXaa: 0.0 ± 0.0
Cys
0.377CysAla: 0.377 ± 0.022
0.096CysCys: 0.096 ± 0.012
0.332CysAsp: 0.332 ± 0.021
0.364CysGlu: 0.364 ± 0.028
0.158CysPhe: 0.158 ± 0.016
0.934CysGly: 0.934 ± 0.045
0.138CysHis: 0.138 ± 0.015
0.518CysIle: 0.518 ± 0.029
0.286CysLys: 0.286 ± 0.019
0.532CysLeu: 0.532 ± 0.032
0.166CysMet: 0.166 ± 0.016
0.329CysAsn: 0.329 ± 0.023
0.722CysPro: 0.722 ± 0.037
0.189CysGln: 0.189 ± 0.016
0.463CysArg: 0.463 ± 0.024
0.478CysSer: 0.478 ± 0.03
0.413CysThr: 0.413 ± 0.024
0.562CysVal: 0.562 ± 0.028
0.107CysTrp: 0.107 ± 0.01
0.213CysTyr: 0.213 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
3.327AspAla: 3.327 ± 0.076
0.412AspCys: 0.412 ± 0.025
2.352AspAsp: 2.352 ± 0.061
3.804AspGlu: 3.804 ± 0.082
1.803AspPhe: 1.803 ± 0.049
3.16AspGly: 3.16 ± 0.075
0.619AspHis: 0.619 ± 0.029
3.692AspIle: 3.692 ± 0.078
2.456AspLys: 2.456 ± 0.065
5.441AspLeu: 5.441 ± 0.09
1.06AspMet: 1.06 ± 0.038
1.597AspAsn: 1.597 ± 0.051
2.537AspPro: 2.537 ± 0.061
0.717AspGln: 0.717 ± 0.031
2.669AspArg: 2.669 ± 0.06
2.18AspSer: 2.18 ± 0.062
1.762AspThr: 1.762 ± 0.051
5.35AspVal: 5.35 ± 0.108
0.762AspTrp: 0.762 ± 0.034
2.76AspTyr: 2.76 ± 0.064
0.0AspXaa: 0.0 ± 0.0
Glu
4.516GluAla: 4.516 ± 0.086
0.429GluCys: 0.429 ± 0.026
3.149GluAsp: 3.149 ± 0.085
4.748GluGlu: 4.748 ± 0.116
2.564GluPhe: 2.564 ± 0.054
3.887GluGly: 3.887 ± 0.087
0.88GluHis: 0.88 ± 0.036
4.113GluIle: 4.113 ± 0.09
2.534GluLys: 2.534 ± 0.073
8.435GluLeu: 8.435 ± 0.118
1.136GluMet: 1.136 ± 0.043
1.879GluAsn: 1.879 ± 0.058
2.394GluPro: 2.394 ± 0.06
0.975GluGln: 0.975 ± 0.037
3.604GluArg: 3.604 ± 0.095
2.778GluSer: 2.778 ± 0.061
2.215GluThr: 2.215 ± 0.059
6.427GluVal: 6.427 ± 0.11
0.701GluTrp: 0.701 ± 0.034
2.602GluTyr: 2.602 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
2.066PheAla: 2.066 ± 0.059
0.192PheCys: 0.192 ± 0.018
1.707PheAsp: 1.707 ± 0.049
1.824PheGlu: 1.824 ± 0.056
1.027PhePhe: 1.027 ± 0.037
2.713PheGly: 2.713 ± 0.066
0.488PheHis: 0.488 ± 0.025
3.493PheIle: 3.493 ± 0.073
1.695PheLys: 1.695 ± 0.05
3.293PheLeu: 3.293 ± 0.068
1.16PheMet: 1.16 ± 0.043
1.922PheAsn: 1.922 ± 0.057
1.198PhePro: 1.198 ± 0.046
0.632PheGln: 0.632 ± 0.028
2.356PheArg: 2.356 ± 0.061
2.14PheSer: 2.14 ± 0.054
2.174PheThr: 2.174 ± 0.058
2.764PheVal: 2.764 ± 0.068
0.361PheTrp: 0.361 ± 0.023
1.263PheTyr: 1.263 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
4.605GlyAla: 4.605 ± 0.094
0.595GlyCys: 0.595 ± 0.03
3.781GlyAsp: 3.781 ± 0.084
4.011GlyGlu: 4.011 ± 0.078
3.482GlyPhe: 3.482 ± 0.075
5.343GlyGly: 5.343 ± 0.115
1.132GlyHis: 1.132 ± 0.041
6.887GlyIle: 6.887 ± 0.116
3.955GlyLys: 3.955 ± 0.076
7.958GlyLeu: 7.958 ± 0.148
1.809GlyMet: 1.809 ± 0.053
3.249GlyAsn: 3.249 ± 0.07
2.792GlyPro: 2.792 ± 0.065
1.353GlyGln: 1.353 ± 0.054
4.289GlyArg: 4.289 ± 0.098
4.715GlySer: 4.715 ± 0.097
3.369GlyThr: 3.369 ± 0.079
7.002GlyVal: 7.002 ± 0.115
1.061GlyTrp: 1.061 ± 0.046
3.378GlyTyr: 3.378 ± 0.071
0.0GlyXaa: 0.0 ± 0.0
His
1.095HisAla: 1.095 ± 0.04
0.152HisCys: 0.152 ± 0.014
0.707HisAsp: 0.707 ± 0.035
1.002HisGlu: 1.002 ± 0.037
0.468HisPhe: 0.468 ± 0.025
1.481HisGly: 1.481 ± 0.043
0.281HisHis: 0.281 ± 0.021
0.972HisIle: 0.972 ± 0.041
0.474HisLys: 0.474 ± 0.027
1.228HisLeu: 1.228 ± 0.041
0.401HisMet: 0.401 ± 0.025
0.484HisAsn: 0.484 ± 0.026
0.753HisPro: 0.753 ± 0.035
0.251HisGln: 0.251 ± 0.02
0.928HisArg: 0.928 ± 0.036
0.679HisSer: 0.679 ± 0.031
0.6HisThr: 0.6 ± 0.028
1.539HisVal: 1.539 ± 0.047
0.231HisTrp: 0.231 ± 0.02
0.753HisTyr: 0.753 ± 0.035
0.0HisXaa: 0.0 ± 0.0
Ile
6.511IleAla: 6.511 ± 0.112
0.418IleCys: 0.418 ± 0.028
4.938IleAsp: 4.938 ± 0.093
4.99IleGlu: 4.99 ± 0.093
2.032IlePhe: 2.032 ± 0.058
6.015IleGly: 6.015 ± 0.117
1.266IleHis: 1.266 ± 0.041
7.512IleIle: 7.512 ± 0.12
5.075IleLys: 5.075 ± 0.096
6.669IleLeu: 6.669 ± 0.121
2.29IleMet: 2.29 ± 0.06
5.702IleAsn: 5.702 ± 0.109
4.069IlePro: 4.069 ± 0.094
1.367IleGln: 1.367 ± 0.048
5.256IleArg: 5.256 ± 0.092
5.13IleSer: 5.13 ± 0.101
5.322IleThr: 5.322 ± 0.107
6.138IleVal: 6.138 ± 0.096
0.66IleTrp: 0.66 ± 0.032
3.392IleTyr: 3.392 ± 0.08
0.0IleXaa: 0.0 ± 0.0
Lys
4.171LysAla: 4.171 ± 0.08
0.499LysCys: 0.499 ± 0.031
2.517LysAsp: 2.517 ± 0.071
3.23LysGlu: 3.23 ± 0.083
1.452LysPhe: 1.452 ± 0.052
2.981LysGly: 2.981 ± 0.07
0.666LysHis: 0.666 ± 0.035
2.974LysIle: 2.974 ± 0.072
1.61LysLys: 1.61 ± 0.061
5.233LysLeu: 5.233 ± 0.092
1.088LysMet: 1.088 ± 0.042
1.321LysAsn: 1.321 ± 0.047
2.828LysPro: 2.828 ± 0.061
0.904LysGln: 0.904 ± 0.032
2.538LysArg: 2.538 ± 0.067
2.51LysSer: 2.51 ± 0.059
1.896LysThr: 1.896 ± 0.051
4.54LysVal: 4.54 ± 0.095
0.578LysTrp: 0.578 ± 0.027
2.825LysTyr: 2.825 ± 0.074
0.0LysXaa: 0.0 ± 0.0
Leu
7.867LeuAla: 7.867 ± 0.129
0.566LeuCys: 0.566 ± 0.031
4.553LeuAsp: 4.553 ± 0.09
5.102LeuGlu: 5.102 ± 0.104
3.258LeuPhe: 3.258 ± 0.084
8.693LeuGly: 8.693 ± 0.12
1.321LeuHis: 1.321 ± 0.041
10.095LeuIle: 10.095 ± 0.159
4.504LeuLys: 4.504 ± 0.083
10.298LeuLeu: 10.298 ± 0.174
3.505LeuMet: 3.505 ± 0.076
5.14LeuAsn: 5.14 ± 0.093
4.467LeuPro: 4.467 ± 0.081
1.511LeuGln: 1.511 ± 0.052
7.853LeuArg: 7.853 ± 0.129
7.646LeuSer: 7.646 ± 0.123
5.766LeuThr: 5.766 ± 0.094
7.984LeuVal: 7.984 ± 0.107
1.14LeuTrp: 1.14 ± 0.044
3.595LeuTyr: 3.595 ± 0.077
0.0LeuXaa: 0.0 ± 0.0
Met
2.349MetAla: 2.349 ± 0.069
0.157MetCys: 0.157 ± 0.017
1.295MetAsp: 1.295 ± 0.045
1.474MetGlu: 1.474 ± 0.052
0.748MetPhe: 0.748 ± 0.034
2.417MetGly: 2.417 ± 0.059
0.464MetHis: 0.464 ± 0.025
1.991MetIle: 1.991 ± 0.056
1.201MetLys: 1.201 ± 0.051
2.413MetLeu: 2.413 ± 0.06
0.618MetMet: 0.618 ± 0.031
1.314MetAsn: 1.314 ± 0.039
1.558MetPro: 1.558 ± 0.044
0.447MetGln: 0.447 ± 0.028
1.858MetArg: 1.858 ± 0.05
2.167MetSer: 2.167 ± 0.051
1.199MetThr: 1.199 ± 0.044
2.125MetVal: 2.125 ± 0.05
0.255MetTrp: 0.255 ± 0.021
0.95MetTyr: 0.95 ± 0.041
0.0MetXaa: 0.0 ± 0.0
Asn
3.8AsnAla: 3.8 ± 0.076
0.409AsnCys: 0.409 ± 0.026
2.291AsnAsp: 2.291 ± 0.061
3.547AsnGlu: 3.547 ± 0.076
1.116AsnPhe: 1.116 ± 0.036
3.199AsnGly: 3.199 ± 0.068
0.533AsnHis: 0.533 ± 0.026
3.17AsnIle: 3.17 ± 0.064
2.118AsnLys: 2.118 ± 0.058
3.389AsnLeu: 3.389 ± 0.076
0.889AsnMet: 0.889 ± 0.036
2.145AsnAsn: 2.145 ± 0.064
2.438AsnPro: 2.438 ± 0.066
0.887AsnGln: 0.887 ± 0.038
2.153AsnArg: 2.153 ± 0.053
2.178AsnSer: 2.178 ± 0.06
2.269AsnThr: 2.269 ± 0.064
4.028AsnVal: 4.028 ± 0.086
0.588AsnTrp: 0.588 ± 0.028
2.64AsnTyr: 2.64 ± 0.074
0.0AsnXaa: 0.0 ± 0.0
Pro
2.324ProAla: 2.324 ± 0.065
0.385ProCys: 0.385 ± 0.024
2.004ProAsp: 2.004 ± 0.054
2.74ProGlu: 2.74 ± 0.075
1.566ProPhe: 1.566 ± 0.049
3.574ProGly: 3.574 ± 0.081
0.777ProHis: 0.777 ± 0.027
3.887ProIle: 3.887 ± 0.073
1.758ProLys: 1.758 ± 0.043
4.247ProLeu: 4.247 ± 0.08
1.339ProMet: 1.339 ± 0.05
1.83ProAsn: 1.83 ± 0.063
2.408ProPro: 2.408 ± 0.095
1.189ProGln: 1.189 ± 0.052
2.945ProArg: 2.945 ± 0.07
3.201ProSer: 3.201 ± 0.076
2.478ProThr: 2.478 ± 0.073
3.358ProVal: 3.358 ± 0.081
0.742ProTrp: 0.742 ± 0.037
2.164ProTyr: 2.164 ± 0.067
0.0ProXaa: 0.0 ± 0.0
Gln
1.501GlnAla: 1.501 ± 0.053
0.229GlnCys: 0.229 ± 0.019
0.679GlnAsp: 0.679 ± 0.03
1.189GlnGlu: 1.189 ± 0.05
0.768GlnPhe: 0.768 ± 0.032
1.537GlnGly: 1.537 ± 0.043
0.217GlnHis: 0.217 ± 0.017
0.972GlnIle: 0.972 ± 0.039
0.498GlnLys: 0.498 ± 0.028
2.377GlnLeu: 2.377 ± 0.062
0.521GlnMet: 0.521 ± 0.029
0.543GlnAsn: 0.543 ± 0.025
0.863GlnPro: 0.863 ± 0.041
0.635GlnGln: 0.635 ± 0.04
1.068GlnArg: 1.068 ± 0.038
1.188GlnSer: 1.188 ± 0.052
0.689GlnThr: 0.689 ± 0.033
2.016GlnVal: 2.016 ± 0.057
0.305GlnTrp: 0.305 ± 0.022
1.161GlnTyr: 1.161 ± 0.053
0.0GlnXaa: 0.0 ± 0.0
Arg
4.437ArgAla: 4.437 ± 0.09
0.516ArgCys: 0.516 ± 0.031
3.845ArgAsp: 3.845 ± 0.081
5.088ArgGlu: 5.088 ± 0.109
2.489ArgPhe: 2.489 ± 0.067
4.866ArgGly: 4.866 ± 0.097
1.026ArgHis: 1.026 ± 0.038
4.525ArgIle: 4.525 ± 0.078
2.709ArgLys: 2.709 ± 0.071
6.689ArgLeu: 6.689 ± 0.112
1.338ArgMet: 1.338 ± 0.042
2.445ArgAsn: 2.445 ± 0.057
2.383ArgPro: 2.383 ± 0.069
1.283ArgGln: 1.283 ± 0.039
5.137ArgArg: 5.137 ± 0.105
3.292ArgSer: 3.292 ± 0.075
2.083ArgThr: 2.083 ± 0.054
6.142ArgVal: 6.142 ± 0.133
0.844ArgTrp: 0.844 ± 0.033
2.551ArgTyr: 2.551 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
3.436SerAla: 3.436 ± 0.078
0.418SerCys: 0.418 ± 0.024
2.767SerAsp: 2.767 ± 0.06
3.024SerGlu: 3.024 ± 0.064
2.009SerPhe: 2.009 ± 0.057
4.56SerGly: 4.56 ± 0.088
0.807SerHis: 0.807 ± 0.039
6.156SerIle: 6.156 ± 0.105
2.959SerLys: 2.959 ± 0.077
6.693SerLeu: 6.693 ± 0.122
2.077SerMet: 2.077 ± 0.056
2.777SerAsn: 2.777 ± 0.066
2.55SerPro: 2.55 ± 0.069
1.333SerGln: 1.333 ± 0.047
3.939SerArg: 3.939 ± 0.075
4.017SerSer: 4.017 ± 0.104
3.338SerThr: 3.338 ± 0.081
4.576SerVal: 4.576 ± 0.081
0.72SerTrp: 0.72 ± 0.035
2.068SerTyr: 2.068 ± 0.064
0.0SerXaa: 0.0 ± 0.0
Thr
3.678ThrAla: 3.678 ± 0.101
0.416ThrCys: 0.416 ± 0.024
1.806ThrAsp: 1.806 ± 0.054
2.387ThrGlu: 2.387 ± 0.051
1.518ThrPhe: 1.518 ± 0.053
3.749ThrGly: 3.749 ± 0.084
0.838ThrHis: 0.838 ± 0.033
4.097ThrIle: 4.097 ± 0.086
2.078ThrLys: 2.078 ± 0.051
4.964ThrLeu: 4.964 ± 0.089
1.381ThrMet: 1.381 ± 0.045
1.985ThrAsn: 1.985 ± 0.066
2.519ThrPro: 2.519 ± 0.083
1.127ThrGln: 1.127 ± 0.043
2.538ThrArg: 2.538 ± 0.066
2.737ThrSer: 2.737 ± 0.077
2.778ThrThr: 2.778 ± 0.095
4.308ThrVal: 4.308 ± 0.111
0.832ThrTrp: 0.832 ± 0.042
2.633ThrTyr: 2.633 ± 0.062
0.0ThrXaa: 0.0 ± 0.0
Val
5.505ValAla: 5.505 ± 0.099
0.545ValCys: 0.545 ± 0.032
4.545ValAsp: 4.545 ± 0.096
4.837ValGlu: 4.837 ± 0.096
3.159ValPhe: 3.159 ± 0.072
6.389ValGly: 6.389 ± 0.119
1.251ValHis: 1.251 ± 0.051
9.055ValIle: 9.055 ± 0.123
5.367ValLys: 5.367 ± 0.091
8.811ValLeu: 8.811 ± 0.121
2.437ValMet: 2.437 ± 0.06
4.763ValAsn: 4.763 ± 0.079
3.602ValPro: 3.602 ± 0.07
1.325ValGln: 1.325 ± 0.039
6.049ValArg: 6.049 ± 0.115
5.599ValSer: 5.599 ± 0.097
4.371ValThr: 4.371 ± 0.097
7.815ValVal: 7.815 ± 0.127
0.873ValTrp: 0.873 ± 0.031
3.378ValTyr: 3.378 ± 0.072
0.0ValXaa: 0.0 ± 0.0
Trp
0.872TrpAla: 0.872 ± 0.039
0.097TrpCys: 0.097 ± 0.012
0.714TrpAsp: 0.714 ± 0.031
0.553TrpGlu: 0.553 ± 0.032
0.641TrpPhe: 0.641 ± 0.032
0.989TrpGly: 0.989 ± 0.04
0.209TrpHis: 0.209 ± 0.018
0.84TrpIle: 0.84 ± 0.038
0.409TrpLys: 0.409 ± 0.024
1.563TrpLeu: 1.563 ± 0.055
0.269TrpMet: 0.269 ± 0.022
0.494TrpAsn: 0.494 ± 0.027
0.48TrpPro: 0.48 ± 0.028
0.367TrpGln: 0.367 ± 0.034
0.934TrpArg: 0.934 ± 0.037
0.862TrpSer: 0.862 ± 0.041
0.471TrpThr: 0.471 ± 0.028
1.287TrpVal: 1.287 ± 0.046
0.258TrpTrp: 0.258 ± 0.021
0.556TrpTyr: 0.556 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.175TyrAla: 3.175 ± 0.076
0.371TyrCys: 0.371 ± 0.026
2.056TyrAsp: 2.056 ± 0.06
2.521TyrGlu: 2.521 ± 0.062
1.513TyrPhe: 1.513 ± 0.045
3.829TyrGly: 3.829 ± 0.074
0.543TyrHis: 0.543 ± 0.027
3.213TyrIle: 3.213 ± 0.072
1.435TyrLys: 1.435 ± 0.054
5.02TyrLeu: 5.02 ± 0.094
1.18TyrMet: 1.18 ± 0.042
1.64TyrAsn: 1.64 ± 0.056
2.059TyrPro: 2.059 ± 0.059
0.816TyrGln: 0.816 ± 0.041
2.413TyrArg: 2.413 ± 0.062
2.575TyrSer: 2.575 ± 0.066
2.16TyrThr: 2.16 ± 0.069
4.91TyrVal: 4.91 ± 0.09
0.76TyrTrp: 0.76 ± 0.035
2.214TyrTyr: 2.214 ± 0.083
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2493 proteins (708751 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski