Fervidicella metallireducens AeB
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2800 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A017RXM9|A0A017RXM9_9CLOT Phosphoribosylaminoimidazole-succinocarboxamide synthase OS=Fervidicella metallireducens AeB OX=1403537 GN=purC PE=3 SV=1
MM1 pKa = 7.13 NSCMIFWVPIYY12 pKa = 9.37 PQNSKK17 pKa = 10.42 NLNIYY22 pKa = 10.8 RR23 pKa = 11.84 NDD25 pKa = 3.14 TTLAEE30 pKa = 4.3 EE31 pKa = 4.05 EE32 pKa = 4.69 TEE34 pKa = 4.28 NSLEE38 pKa = 3.98 MLFDD42 pKa = 5.2 EE43 pKa = 5.19 IDD45 pKa = 3.38 EE46 pKa = 4.67 EE47 pKa = 4.97 YY48 pKa = 10.35 ISQSLGQQ55 pKa = 3.57
Molecular weight: 6.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.848
IPC2_protein 3.821
IPC_protein 3.605
Toseland 3.478
ProMoST 3.783
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.503
Rodwell 3.478
Grimsley 3.401
Solomon 3.528
Lehninger 3.49
Nozaki 3.745
DTASelect 3.795
Thurlkill 3.541
EMBOSS 3.516
Sillero 3.732
Patrickios 0.477
IPC_peptide 3.541
IPC2_peptide 3.706
IPC2.peptide.svr19 3.69
Protein with the highest isoelectric point:
>tr|A0A017RXL7|A0A017RXL7_9CLOT Flavodoxin OS=Fervidicella metallireducens AeB OX=1403537 GN=Q428_04130 PE=4 SV=1
MM1 pKa = 7.45 LRR3 pKa = 11.84 TYY5 pKa = 9.8 QPKK8 pKa = 9.46 KK9 pKa = 8.28 RR10 pKa = 11.84 QRR12 pKa = 11.84 KK13 pKa = 8.39 KK14 pKa = 8.49 EE15 pKa = 3.5 HH16 pKa = 6.11 GFRR19 pKa = 11.84 KK20 pKa = 9.69 RR21 pKa = 11.84 MKK23 pKa = 8.73 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 LVLQRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.92 GRR39 pKa = 11.84 KK40 pKa = 9.02 RR41 pKa = 11.84 LTAA44 pKa = 4.18
Molecular weight: 5.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.376
IPC2_protein 10.95
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.544
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.281
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 12.003
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.058
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2800
0
2800
781929
29
2410
279.3
31.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.729 ± 0.051
1.2 ± 0.021
5.418 ± 0.035
7.436 ± 0.052
4.395 ± 0.036
6.455 ± 0.046
1.384 ± 0.018
9.993 ± 0.053
9.002 ± 0.048
8.987 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.641 ± 0.023
5.915 ± 0.047
2.945 ± 0.028
2.428 ± 0.02
3.822 ± 0.032
5.929 ± 0.037
4.865 ± 0.04
6.593 ± 0.043
0.668 ± 0.012
4.195 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here