Bacillus sp. SGD-V-76

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3896 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0V8JI23|A0A0V8JI23_9BACI Uncharacterized protein OS=Bacillus sp. SGD-V-76 OX=1414648 GN=AS180_17220 PE=4 SV=1
MM1 pKa = 7.44NSKK4 pKa = 10.55KK5 pKa = 10.54LGSFHH10 pKa = 6.48VTDD13 pKa = 4.32LQLIISDD20 pKa = 3.65PCYY23 pKa = 10.21PIEE26 pKa = 4.74CAVDD30 pKa = 3.85DD31 pKa = 4.7SDD33 pKa = 4.82DD34 pKa = 3.86FAWRR38 pKa = 11.84LEE40 pKa = 3.9PAKK43 pKa = 10.42RR44 pKa = 11.84GEE46 pKa = 3.77WTATVFYY53 pKa = 10.11EE54 pKa = 5.42DD55 pKa = 5.27DD56 pKa = 3.6FTISKK61 pKa = 10.23LVATHH66 pKa = 6.76TDD68 pKa = 3.87FKK70 pKa = 10.44MTDD73 pKa = 2.5NWIDD77 pKa = 3.17TGKK80 pKa = 10.61EE81 pKa = 3.83VGVDD85 pKa = 3.37SAKK88 pKa = 10.95AGIFDD93 pKa = 3.67ASVYY97 pKa = 10.74GRR99 pKa = 11.84DD100 pKa = 3.56DD101 pKa = 5.07AITYY105 pKa = 8.2EE106 pKa = 4.14VEE108 pKa = 4.1NVHH111 pKa = 6.75EE112 pKa = 4.38INMDD116 pKa = 3.78EE117 pKa = 4.41EE118 pKa = 4.47GLTYY122 pKa = 10.64YY123 pKa = 9.31VACCDD128 pKa = 3.44AVASSEE134 pKa = 4.28YY135 pKa = 10.23EE136 pKa = 3.78AGIVPGGVVSMAGIGDD152 pKa = 4.69GYY154 pKa = 10.56YY155 pKa = 9.79PAYY158 pKa = 9.78VQYY161 pKa = 11.16DD162 pKa = 3.75NQEE165 pKa = 3.73IAAVLLDD172 pKa = 3.64FWFDD176 pKa = 3.6EE177 pKa = 4.46EE178 pKa = 4.67EE179 pKa = 4.11EE180 pKa = 4.22

Molecular weight: 20.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0V8JN56|A0A0V8JN56_9BACI 2-oxoisovalerate dehydrogenase OS=Bacillus sp. SGD-V-76 OX=1414648 GN=AS180_07860 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.21QPNRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.75RR12 pKa = 11.84SKK14 pKa = 9.38VHH16 pKa = 5.87GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.82VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3896

0

3896

1084126

26

3139

278.3

31.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.009 ± 0.041

0.733 ± 0.011

4.902 ± 0.03

7.541 ± 0.047

4.445 ± 0.03

6.635 ± 0.042

2.166 ± 0.022

7.493 ± 0.041

7.065 ± 0.037

9.84 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.705 ± 0.019

4.334 ± 0.029

3.467 ± 0.023

4.121 ± 0.03

3.897 ± 0.029

6.107 ± 0.024

5.55 ± 0.031

7.417 ± 0.034

0.955 ± 0.013

3.618 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski