Avian metaavulavirus 8
Average proteome isoelectric point is 5.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C5I0V2|C5I0V2_9MONO Phosphoprotein OS=Avian metaavulavirus 8 OX=2560318 GN=P PE=4 SV=1
MM1 pKa = 7.39 SNIASSLEE9 pKa = 4.09 NIVEE13 pKa = 3.88 QDD15 pKa = 3.05 SRR17 pKa = 11.84 KK18 pKa = 6.29 TTWRR22 pKa = 11.84 AIFRR26 pKa = 11.84 WSVLLITTGCLALSIVSIVQIGNLKK51 pKa = 9.78 IPSVGDD57 pKa = 3.38 LADD60 pKa = 3.74 EE61 pKa = 4.38 VVTPLKK67 pKa = 8.49 TTLSDD72 pKa = 3.5 TLRR75 pKa = 11.84 NPINQINDD83 pKa = 2.72 IFRR86 pKa = 11.84 IVALDD91 pKa = 3.39 IPLQVTSIQKK101 pKa = 10.27 DD102 pKa = 3.48 LASQFSMLIDD112 pKa = 3.39 SLNAIKK118 pKa = 10.61 LGNGTNLIIPTSDD131 pKa = 2.77 KK132 pKa = 10.71 EE133 pKa = 4.18 YY134 pKa = 10.99 AGGIGNPVFTVDD146 pKa = 3.25 AGGSIGFKK154 pKa = 9.57 QFSLIEE160 pKa = 4.29 HH161 pKa = 7.17 PSFIAGPTTTRR172 pKa = 11.84 GCTRR176 pKa = 11.84 IPTFHH181 pKa = 6.76 MSEE184 pKa = 4.21 SHH186 pKa = 5.05 WCYY189 pKa = 9.94 SHH191 pKa = 7.04 NIIAAGCQDD200 pKa = 3.49 ASASSMYY207 pKa = 10.46 ISMGVLHH214 pKa = 6.54 VSSSGTPIFLTTASEE229 pKa = 4.59 LIDD232 pKa = 4.71 DD233 pKa = 4.47 GVNRR237 pKa = 11.84 KK238 pKa = 9.16 SCSIVATQFGCDD250 pKa = 3.31 ILCSIVIEE258 pKa = 4.95 KK259 pKa = 10.73 EE260 pKa = 3.82 GDD262 pKa = 3.87 DD263 pKa = 3.97 YY264 pKa = 11.52 WSDD267 pKa = 3.24 TPTPMRR273 pKa = 11.84 HH274 pKa = 5.49 GRR276 pKa = 11.84 FSFNGSFVEE285 pKa = 4.39 TEE287 pKa = 4.37 LPVSSMFSSFSANYY301 pKa = 8.0 PAVGSGEE308 pKa = 4.07 IVKK311 pKa = 10.47 DD312 pKa = 3.88 RR313 pKa = 11.84 ILFPIYY319 pKa = 10.55 GGIKK323 pKa = 7.85 QTSPEE328 pKa = 3.75 FTEE331 pKa = 4.16 LVKK334 pKa = 11.22 YY335 pKa = 10.5 GLFVSTPTTVCQSSWTYY352 pKa = 11.11 DD353 pKa = 3.39 QVKK356 pKa = 9.54 AAYY359 pKa = 9.45 RR360 pKa = 11.84 PDD362 pKa = 4.15 YY363 pKa = 10.62 ISGRR367 pKa = 11.84 FWAQVILSCALDD379 pKa = 3.78 AVDD382 pKa = 5.94 LSSCIVKK389 pKa = 10.13 IMNSSTVMMAAEE401 pKa = 4.06 GRR403 pKa = 11.84 IIKK406 pKa = 10.18 IGIDD410 pKa = 3.03 YY411 pKa = 9.78 FYY413 pKa = 10.53 YY414 pKa = 10.3 QRR416 pKa = 11.84 SSSWWPLAFVTKK428 pKa = 10.57 LDD430 pKa = 3.92 PQEE433 pKa = 4.79 LADD436 pKa = 4.56 TNSIWLTNSIPIPQSKK452 pKa = 9.59 FPRR455 pKa = 11.84 PSYY458 pKa = 10.67 SEE460 pKa = 4.17 NYY462 pKa = 6.58 CTKK465 pKa = 10.29 PAVCPATCVTGVYY478 pKa = 10.43 SDD480 pKa = 3.44 IWPLTSSSSLPSIIWIGQYY499 pKa = 10.79 LDD501 pKa = 3.82 APVGRR506 pKa = 11.84 TYY508 pKa = 11.06 PRR510 pKa = 11.84 FGIANQSHH518 pKa = 6.99 WYY520 pKa = 8.34 LQEE523 pKa = 5.89 DD524 pKa = 3.92 ILPTSTASAYY534 pKa = 8.49 STTTCFKK541 pKa = 8.14 NTARR545 pKa = 11.84 NRR547 pKa = 11.84 VFCVTIAEE555 pKa = 4.31 FADD558 pKa = 3.97 GLFGEE563 pKa = 4.55 YY564 pKa = 9.99 RR565 pKa = 11.84 ITPQLYY571 pKa = 9.5 EE572 pKa = 3.99 LVRR575 pKa = 11.84 NNN577 pKa = 3.26
Molecular weight: 63.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.063
IPC2_protein 5.258
IPC_protein 5.219
Toseland 5.232
ProMoST 5.423
Dawson 5.283
Bjellqvist 5.385
Wikipedia 5.194
Rodwell 5.207
Grimsley 5.181
Solomon 5.283
Lehninger 5.245
Nozaki 5.436
DTASelect 5.626
Thurlkill 5.308
EMBOSS 5.296
Sillero 5.499
Patrickios 3.236
IPC_peptide 5.296
IPC2_peptide 5.499
IPC2.peptide.svr19 5.459
Protein with the highest isoelectric point:
>tr|C5I0V8|C5I0V8_9MONO RNA-directed RNA polymerase L OS=Avian metaavulavirus 8 OX=2560318 GN=L PE=3 SV=1
MM1 pKa = 8.04 AYY3 pKa = 6.79 TTLKK7 pKa = 10.58 LWVDD11 pKa = 3.86 EE12 pKa = 4.58 GDD14 pKa = 3.52 MSSSLLSFPLVLKK27 pKa = 9.21 EE28 pKa = 4.05 TDD30 pKa = 3.02 RR31 pKa = 11.84 GTKK34 pKa = 9.82 KK35 pKa = 10.22 LQPQVRR41 pKa = 11.84 VDD43 pKa = 4.33 SIGDD47 pKa = 3.38 VQNAKK52 pKa = 9.96 EE53 pKa = 3.97 SSIFVTLYY61 pKa = 11.23 GFIQAIKK68 pKa = 9.73 EE69 pKa = 3.89 NSDD72 pKa = 3.02 RR73 pKa = 11.84 SKK75 pKa = 10.9 FFHH78 pKa = 6.92 PKK80 pKa = 10.37 DD81 pKa = 3.51 DD82 pKa = 4.65 FKK84 pKa = 11.56 PEE86 pKa = 3.84 TVTAGLVVVGAIRR99 pKa = 11.84 MMADD103 pKa = 2.93 VNTISNDD110 pKa = 3.06 ALALEE115 pKa = 4.32 ITVKK119 pKa = 10.55 KK120 pKa = 10.59 SATSQEE126 pKa = 3.74 KK127 pKa = 6.8 MTVMFHH133 pKa = 6.8 NSPPSLRR140 pKa = 11.84 TAITIRR146 pKa = 11.84 AGGFISNADD155 pKa = 3.83 EE156 pKa = 4.63 NIKK159 pKa = 10.53 CASKK163 pKa = 9.27 LTAGVQYY170 pKa = 10.19 IFRR173 pKa = 11.84 PMFVSITKK181 pKa = 9.55 LHH183 pKa = 5.95 NGKK186 pKa = 10.22 LYY188 pKa = 10.36 RR189 pKa = 11.84 VPKK192 pKa = 9.32 SIHH195 pKa = 6.47 SISSTLLYY203 pKa = 10.91 SVMLEE208 pKa = 3.83 VGFKK212 pKa = 10.35 VDD214 pKa = 3.07 IGKK217 pKa = 8.82 DD218 pKa = 3.26 HH219 pKa = 7.14 PQAKK223 pKa = 8.32 MLKK226 pKa = 9.34 RR227 pKa = 11.84 VTIGDD232 pKa = 3.87 ADD234 pKa = 4.42 TYY236 pKa = 10.93 WGFAWFHH243 pKa = 6.12 LCNFKK248 pKa = 9.49 KK249 pKa = 9.27 TSSKK253 pKa = 10.71 GKK255 pKa = 9.35 PRR257 pKa = 11.84 TLDD260 pKa = 3.28 EE261 pKa = 5.04 LRR263 pKa = 11.84 TKK265 pKa = 9.75 VKK267 pKa = 10.91 NMGLKK272 pKa = 10.58 LEE274 pKa = 4.77 LHH276 pKa = 6.76 DD277 pKa = 4.35 LWGPTIVVQITGKK290 pKa = 9.42 SSKK293 pKa = 9.55 YY294 pKa = 8.99 AQGFFSSNGTCCLPISRR311 pKa = 11.84 SAPEE315 pKa = 4.14 LGKK318 pKa = 10.77 LLWSCSATIGDD329 pKa = 4.04 ATVVIQSSEE338 pKa = 3.84 KK339 pKa = 11.01 GEE341 pKa = 4.13 LLRR344 pKa = 11.84 SDD346 pKa = 3.95 DD347 pKa = 3.93 LEE349 pKa = 4.55 IRR351 pKa = 11.84 GAVASKK357 pKa = 10.37 KK358 pKa = 10.03 GRR360 pKa = 11.84 LSSFHH365 pKa = 6.72 PFKK368 pKa = 10.86 KK369 pKa = 10.55
Molecular weight: 40.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.844
IPC2_protein 8.829
IPC_protein 8.697
Toseland 9.75
ProMoST 9.355
Dawson 9.911
Bjellqvist 9.545
Wikipedia 10.014
Rodwell 10.526
Grimsley 9.94
Solomon 9.926
Lehninger 9.911
Nozaki 9.809
DTASelect 9.502
Thurlkill 9.78
EMBOSS 10.131
Sillero 9.838
Patrickios 10.116
IPC_peptide 9.926
IPC2_peptide 8.024
IPC2.peptide.svr19 7.763
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
2
8
5003
172
2238
625.4
69.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.056 ± 0.869
1.939 ± 0.306
5.297 ± 0.311
5.277 ± 0.458
3.238 ± 0.55
5.177 ± 0.464
1.919 ± 0.414
7.635 ± 0.54
5.237 ± 0.548
10.194 ± 1.205
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.219 ± 0.27
5.017 ± 0.389
4.517 ± 0.591
4.657 ± 0.559
4.617 ± 0.354
10.014 ± 0.328
6.976 ± 0.547
5.956 ± 0.474
1.099 ± 0.185
2.958 ± 0.478
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here