Cryobacterium levicorallinum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Cryobacterium

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3509 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I2XXW3|A0A1I2XXW3_9MICO 50S ribosomal protein L34 OS=Cryobacterium levicorallinum OX=995038 GN=rpmH PE=3 SV=1
MM1 pKa = 7.47HH2 pKa = 7.23KK3 pKa = 10.1RR4 pKa = 11.84YY5 pKa = 8.96FAVPVLLASAALALSGCSAGTTASADD31 pKa = 3.69SADD34 pKa = 3.61VGLKK38 pKa = 10.02VVATTTQVADD48 pKa = 4.33FARR51 pKa = 11.84NVIGDD56 pKa = 3.71ASGVDD61 pKa = 4.26LTQLIQPNQSAHH73 pKa = 6.64SYY75 pKa = 10.52DD76 pKa = 3.94PSAADD81 pKa = 3.61LTALGAADD89 pKa = 3.34VLVINGVGLEE99 pKa = 4.03EE100 pKa = 4.29WLDD103 pKa = 3.76DD104 pKa = 4.98AIVASGFDD112 pKa = 3.82GVTIDD117 pKa = 3.93ADD119 pKa = 3.64EE120 pKa = 5.81GITISEE126 pKa = 4.1VGAGEE131 pKa = 4.08DD132 pKa = 3.52HH133 pKa = 6.75SDD135 pKa = 3.44EE136 pKa = 4.2VAEE139 pKa = 4.63EE140 pKa = 4.37DD141 pKa = 3.93DD142 pKa = 4.65HH143 pKa = 9.79ADD145 pKa = 3.72EE146 pKa = 5.12EE147 pKa = 5.18ADD149 pKa = 3.32AHH151 pKa = 6.79AGEE154 pKa = 5.55AEE156 pKa = 4.12DD157 pKa = 4.36AHH159 pKa = 7.9DD160 pKa = 5.34GGDD163 pKa = 3.41PHH165 pKa = 8.36IWTDD169 pKa = 2.8VHH171 pKa = 5.95NAEE174 pKa = 5.15TIVQTITDD182 pKa = 4.14GLVAADD188 pKa = 4.45SDD190 pKa = 3.78NAANFEE196 pKa = 4.52ANAAAYY202 pKa = 9.01SAQLSALDD210 pKa = 3.34DD211 pKa = 4.6WIRR214 pKa = 11.84TNVEE218 pKa = 4.11TVPEE222 pKa = 4.09SDD224 pKa = 3.56RR225 pKa = 11.84LLVSNHH231 pKa = 6.65DD232 pKa = 3.54ALGYY236 pKa = 7.72FTAAYY241 pKa = 9.53HH242 pKa = 6.12IDD244 pKa = 3.77YY245 pKa = 9.54VGSVIPSFDD254 pKa = 4.23DD255 pKa = 3.41NAEE258 pKa = 4.03PSAAEE263 pKa = 3.85IDD265 pKa = 4.03SLVAAITATGVTAVFSEE282 pKa = 4.56ASLSPKK288 pKa = 8.61TAEE291 pKa = 4.44TIATEE296 pKa = 4.17AGVTVYY302 pKa = 10.77SGDD305 pKa = 3.44DD306 pKa = 3.27ALYY309 pKa = 10.77VDD311 pKa = 4.6SLGPAGSAGDD321 pKa = 3.93TYY323 pKa = 11.22IKK325 pKa = 10.66AQLHH329 pKa = 6.16NSTLMLEE336 pKa = 4.39SWGVTPSALPAEE348 pKa = 4.55LQQ350 pKa = 3.33

Molecular weight: 35.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I3DHF3|A0A1I3DHF3_9MICO SipW-cognate class signal peptide OS=Cryobacterium levicorallinum OX=995038 GN=E3O11_09355 PE=4 SV=1
MM1 pKa = 7.76SIGILLVLAGLYY13 pKa = 10.12LLLMRR18 pKa = 11.84RR19 pKa = 11.84FVLRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84IAQGLPVSGPWHH38 pKa = 5.88FPAWLRR44 pKa = 11.84PRR46 pKa = 11.84VPRR49 pKa = 11.84WARR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84FARR57 pKa = 11.84RR58 pKa = 11.84PRR60 pKa = 11.84RR61 pKa = 11.84PRR63 pKa = 11.84AARR66 pKa = 11.84RR67 pKa = 11.84LRR69 pKa = 11.84HH70 pKa = 5.57TRR72 pKa = 11.84PGPPP76 pKa = 3.11

Molecular weight: 9.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3509

0

3509

1112413

25

2149

317.0

33.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.854 ± 0.053

0.582 ± 0.01

5.798 ± 0.036

5.163 ± 0.036

3.297 ± 0.027

8.634 ± 0.036

1.996 ± 0.02

5.033 ± 0.035

2.315 ± 0.025

10.548 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.864 ± 0.017

2.508 ± 0.025

5.149 ± 0.027

3.008 ± 0.022

6.56 ± 0.041

6.163 ± 0.029

6.447 ± 0.033

8.66 ± 0.034

1.394 ± 0.017

2.026 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski