Amino acid dipepetide frequency for Microbacterium phage Lucky3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.616AlaAla: 11.616 ± 1.184
0.774AlaCys: 0.774 ± 0.229
6.583AlaAsp: 6.583 ± 0.784
6.66AlaGlu: 6.66 ± 0.837
2.71AlaPhe: 2.71 ± 0.425
8.364AlaGly: 8.364 ± 0.854
2.168AlaHis: 2.168 ± 0.435
4.646AlaIle: 4.646 ± 0.624
6.505AlaLys: 6.505 ± 1.089
10.455AlaLeu: 10.455 ± 1.15
2.168AlaMet: 2.168 ± 0.376
3.02AlaAsn: 3.02 ± 0.388
3.95AlaPro: 3.95 ± 0.633
4.337AlaGln: 4.337 ± 0.56
6.428AlaArg: 6.428 ± 0.833
4.956AlaSer: 4.956 ± 0.654
6.273AlaThr: 6.273 ± 0.716
7.434AlaVal: 7.434 ± 0.803
1.781AlaTrp: 1.781 ± 0.439
2.71AlaTyr: 2.71 ± 0.482
0.0AlaXaa: 0.0 ± 0.0
Cys
0.465CysAla: 0.465 ± 0.168
0.0CysCys: 0.0 ± 0.0
0.62CysAsp: 0.62 ± 0.187
0.387CysGlu: 0.387 ± 0.167
0.155CysPhe: 0.155 ± 0.114
0.387CysGly: 0.387 ± 0.164
0.232CysHis: 0.232 ± 0.146
0.077CysIle: 0.077 ± 0.073
0.387CysLys: 0.387 ± 0.158
0.155CysLeu: 0.155 ± 0.099
0.077CysMet: 0.077 ± 0.072
0.077CysAsn: 0.077 ± 0.062
0.542CysPro: 0.542 ± 0.253
0.155CysGln: 0.155 ± 0.107
0.387CysArg: 0.387 ± 0.187
0.542CysSer: 0.542 ± 0.198
0.465CysThr: 0.465 ± 0.169
0.465CysVal: 0.465 ± 0.176
0.232CysTrp: 0.232 ± 0.132
0.232CysTyr: 0.232 ± 0.13
0.0CysXaa: 0.0 ± 0.0
Asp
5.886AspAla: 5.886 ± 0.775
0.697AspCys: 0.697 ± 0.208
4.801AspAsp: 4.801 ± 0.805
4.492AspGlu: 4.492 ± 1.087
2.091AspPhe: 2.091 ± 0.339
4.414AspGly: 4.414 ± 0.618
1.704AspHis: 1.704 ± 0.357
3.407AspIle: 3.407 ± 0.472
2.633AspLys: 2.633 ± 0.481
5.189AspLeu: 5.189 ± 0.516
1.781AspMet: 1.781 ± 0.368
1.394AspAsn: 1.394 ± 0.258
4.724AspPro: 4.724 ± 0.664
2.168AspGln: 2.168 ± 0.358
3.175AspArg: 3.175 ± 0.476
4.104AspSer: 4.104 ± 0.432
3.407AspThr: 3.407 ± 0.656
4.259AspVal: 4.259 ± 0.525
1.859AspTrp: 1.859 ± 0.378
2.401AspTyr: 2.401 ± 0.385
0.0AspXaa: 0.0 ± 0.0
Glu
7.899GluAla: 7.899 ± 0.706
0.465GluCys: 0.465 ± 0.178
5.808GluAsp: 5.808 ± 1.183
5.576GluGlu: 5.576 ± 1.322
1.936GluPhe: 1.936 ± 0.408
4.337GluGly: 4.337 ± 0.587
1.239GluHis: 1.239 ± 0.315
1.704GluIle: 1.704 ± 0.389
1.936GluLys: 1.936 ± 0.41
6.04GluLeu: 6.04 ± 0.734
1.239GluMet: 1.239 ± 0.315
1.936GluAsn: 1.936 ± 0.397
2.013GluPro: 2.013 ± 0.411
3.098GluGln: 3.098 ± 0.532
3.485GluArg: 3.485 ± 0.498
2.71GluSer: 2.71 ± 0.518
3.64GluThr: 3.64 ± 0.385
3.95GluVal: 3.95 ± 0.666
1.781GluTrp: 1.781 ± 0.372
1.626GluTyr: 1.626 ± 0.448
0.0GluXaa: 0.0 ± 0.0
Phe
2.478PheAla: 2.478 ± 0.458
0.232PheCys: 0.232 ± 0.12
1.549PheAsp: 1.549 ± 0.382
1.471PheGlu: 1.471 ± 0.281
0.774PhePhe: 0.774 ± 0.266
2.943PheGly: 2.943 ± 0.413
0.387PheHis: 0.387 ± 0.174
1.084PheIle: 1.084 ± 0.294
1.936PheLys: 1.936 ± 0.381
2.091PheLeu: 2.091 ± 0.333
0.852PheMet: 0.852 ± 0.309
1.549PheAsn: 1.549 ± 0.276
1.394PhePro: 1.394 ± 0.295
1.471PheGln: 1.471 ± 0.335
2.401PheArg: 2.401 ± 0.37
2.091PheSer: 2.091 ± 0.284
2.556PheThr: 2.556 ± 0.504
2.323PheVal: 2.323 ± 0.388
0.387PheTrp: 0.387 ± 0.231
0.697PheTyr: 0.697 ± 0.231
0.0PheXaa: 0.0 ± 0.0
Gly
6.428GlyAla: 6.428 ± 0.835
0.774GlyCys: 0.774 ± 0.213
5.731GlyAsp: 5.731 ± 0.615
4.027GlyGlu: 4.027 ± 0.516
2.943GlyPhe: 2.943 ± 0.495
6.118GlyGly: 6.118 ± 0.899
1.394GlyHis: 1.394 ± 0.401
4.337GlyIle: 4.337 ± 0.592
4.646GlyLys: 4.646 ± 0.586
6.428GlyLeu: 6.428 ± 0.884
2.168GlyMet: 2.168 ± 0.362
2.633GlyAsn: 2.633 ± 0.291
2.788GlyPro: 2.788 ± 0.343
4.104GlyGln: 4.104 ± 0.631
4.337GlyArg: 4.337 ± 0.513
5.498GlySer: 5.498 ± 0.603
6.97GlyThr: 6.97 ± 0.751
5.886GlyVal: 5.886 ± 0.743
1.394GlyTrp: 1.394 ± 0.255
2.168GlyTyr: 2.168 ± 0.422
0.0GlyXaa: 0.0 ± 0.0
His
1.471HisAla: 1.471 ± 0.379
0.0HisCys: 0.0 ± 0.0
0.62HisAsp: 0.62 ± 0.241
1.317HisGlu: 1.317 ± 0.248
0.387HisPhe: 0.387 ± 0.197
1.394HisGly: 1.394 ± 0.361
0.62HisHis: 0.62 ± 0.26
1.007HisIle: 1.007 ± 0.365
1.084HisLys: 1.084 ± 0.3
2.168HisLeu: 2.168 ± 0.407
0.387HisMet: 0.387 ± 0.201
0.852HisAsn: 0.852 ± 0.289
1.007HisPro: 1.007 ± 0.251
0.62HisGln: 0.62 ± 0.206
1.084HisArg: 1.084 ± 0.252
0.697HisSer: 0.697 ± 0.191
0.852HisThr: 0.852 ± 0.231
1.471HisVal: 1.471 ± 0.372
0.465HisTrp: 0.465 ± 0.135
1.084HisTyr: 1.084 ± 0.225
0.0HisXaa: 0.0 ± 0.0
Ile
3.872IleAla: 3.872 ± 0.646
0.31IleCys: 0.31 ± 0.15
3.407IleAsp: 3.407 ± 0.518
2.633IleGlu: 2.633 ± 0.525
0.62IlePhe: 0.62 ± 0.24
3.175IleGly: 3.175 ± 0.735
0.929IleHis: 0.929 ± 0.281
2.556IleIle: 2.556 ± 0.495
1.859IleLys: 1.859 ± 0.383
3.33IleLeu: 3.33 ± 0.468
1.007IleMet: 1.007 ± 0.251
1.781IleAsn: 1.781 ± 0.296
2.246IlePro: 2.246 ± 0.488
2.168IleGln: 2.168 ± 0.402
2.71IleArg: 2.71 ± 0.534
2.788IleSer: 2.788 ± 0.54
4.414IleThr: 4.414 ± 0.655
3.02IleVal: 3.02 ± 0.545
0.852IleTrp: 0.852 ± 0.245
1.239IleTyr: 1.239 ± 0.352
0.0IleXaa: 0.0 ± 0.0
Lys
6.583LysAla: 6.583 ± 1.036
0.232LysCys: 0.232 ± 0.159
2.091LysAsp: 2.091 ± 0.432
2.71LysGlu: 2.71 ± 0.529
1.239LysPhe: 1.239 ± 0.297
4.492LysGly: 4.492 ± 0.558
0.62LysHis: 0.62 ± 0.215
1.626LysIle: 1.626 ± 0.29
3.253LysLys: 3.253 ± 0.697
4.569LysLeu: 4.569 ± 0.606
1.549LysMet: 1.549 ± 0.343
1.317LysAsn: 1.317 ± 0.366
2.943LysPro: 2.943 ± 0.496
1.626LysGln: 1.626 ± 0.303
3.407LysArg: 3.407 ± 0.579
1.394LysSer: 1.394 ± 0.298
3.02LysThr: 3.02 ± 0.519
4.182LysVal: 4.182 ± 0.684
1.084LysTrp: 1.084 ± 0.249
0.774LysTyr: 0.774 ± 0.283
0.0LysXaa: 0.0 ± 0.0
Leu
10.145LeuAla: 10.145 ± 0.856
0.31LeuCys: 0.31 ± 0.184
4.801LeuAsp: 4.801 ± 0.563
5.111LeuGlu: 5.111 ± 0.597
2.013LeuPhe: 2.013 ± 0.295
7.667LeuGly: 7.667 ± 0.748
1.859LeuHis: 1.859 ± 0.269
3.717LeuIle: 3.717 ± 0.973
3.562LeuLys: 3.562 ± 0.506
7.434LeuLeu: 7.434 ± 0.811
1.936LeuMet: 1.936 ± 0.276
2.71LeuAsn: 2.71 ± 0.476
5.189LeuPro: 5.189 ± 0.702
3.33LeuGln: 3.33 ± 0.478
4.646LeuArg: 4.646 ± 0.571
5.421LeuSer: 5.421 ± 0.56
5.421LeuThr: 5.421 ± 0.539
7.125LeuVal: 7.125 ± 0.768
1.007LeuTrp: 1.007 ± 0.236
2.013LeuTyr: 2.013 ± 0.321
0.0LeuXaa: 0.0 ± 0.0
Met
3.175MetAla: 3.175 ± 0.361
0.155MetCys: 0.155 ± 0.102
1.471MetAsp: 1.471 ± 0.329
1.394MetGlu: 1.394 ± 0.371
0.774MetPhe: 0.774 ± 0.224
1.704MetGly: 1.704 ± 0.358
0.31MetHis: 0.31 ± 0.155
1.317MetIle: 1.317 ± 0.361
1.084MetLys: 1.084 ± 0.304
1.781MetLeu: 1.781 ± 0.337
0.387MetMet: 0.387 ± 0.147
1.162MetAsn: 1.162 ± 0.274
0.774MetPro: 0.774 ± 0.252
1.317MetGln: 1.317 ± 0.301
1.007MetArg: 1.007 ± 0.287
2.091MetSer: 2.091 ± 0.354
1.704MetThr: 1.704 ± 0.37
1.162MetVal: 1.162 ± 0.264
0.155MetTrp: 0.155 ± 0.11
0.232MetTyr: 0.232 ± 0.125
0.0MetXaa: 0.0 ± 0.0
Asn
1.859AsnAla: 1.859 ± 0.396
0.0AsnCys: 0.0 ± 0.0
2.168AsnAsp: 2.168 ± 0.438
2.091AsnGlu: 2.091 ± 0.398
0.62AsnPhe: 0.62 ± 0.178
3.098AsnGly: 3.098 ± 0.49
0.465AsnHis: 0.465 ± 0.18
1.471AsnIle: 1.471 ± 0.424
1.626AsnLys: 1.626 ± 0.352
2.401AsnLeu: 2.401 ± 0.371
0.387AsnMet: 0.387 ± 0.174
0.852AsnAsn: 0.852 ± 0.203
2.168AsnPro: 2.168 ± 0.355
1.471AsnGln: 1.471 ± 0.235
1.471AsnArg: 1.471 ± 0.357
2.865AsnSer: 2.865 ± 0.504
2.091AsnThr: 2.091 ± 0.343
2.788AsnVal: 2.788 ± 0.416
0.697AsnTrp: 0.697 ± 0.24
0.774AsnTyr: 0.774 ± 0.252
0.0AsnXaa: 0.0 ± 0.0
Pro
5.111ProAla: 5.111 ± 0.756
0.155ProCys: 0.155 ± 0.16
2.556ProAsp: 2.556 ± 0.466
3.253ProGlu: 3.253 ± 0.576
1.471ProPhe: 1.471 ± 0.309
4.879ProGly: 4.879 ± 0.589
0.697ProHis: 0.697 ± 0.204
1.317ProIle: 1.317 ± 0.217
2.091ProLys: 2.091 ± 0.334
3.33ProLeu: 3.33 ± 0.449
0.852ProMet: 0.852 ± 0.247
2.013ProAsn: 2.013 ± 0.371
1.471ProPro: 1.471 ± 0.321
2.478ProGln: 2.478 ± 0.418
2.323ProArg: 2.323 ± 0.483
3.562ProSer: 3.562 ± 0.496
4.104ProThr: 4.104 ± 0.664
4.801ProVal: 4.801 ± 0.434
0.697ProTrp: 0.697 ± 0.192
1.007ProTyr: 1.007 ± 0.268
0.0ProXaa: 0.0 ± 0.0
Gln
5.498GlnAla: 5.498 ± 0.947
0.077GlnCys: 0.077 ± 0.076
2.401GlnAsp: 2.401 ± 0.493
3.098GlnGlu: 3.098 ± 0.556
0.929GlnPhe: 0.929 ± 0.246
3.098GlnGly: 3.098 ± 0.441
0.929GlnHis: 0.929 ± 0.234
1.626GlnIle: 1.626 ± 0.399
1.704GlnLys: 1.704 ± 0.377
3.64GlnLeu: 3.64 ± 0.431
0.929GlnMet: 0.929 ± 0.225
1.549GlnAsn: 1.549 ± 0.319
1.936GlnPro: 1.936 ± 0.44
2.556GlnGln: 2.556 ± 0.525
2.478GlnArg: 2.478 ± 0.424
2.168GlnSer: 2.168 ± 0.404
2.556GlnThr: 2.556 ± 0.43
3.872GlnVal: 3.872 ± 0.457
0.852GlnTrp: 0.852 ± 0.265
1.007GlnTyr: 1.007 ± 0.222
0.0GlnXaa: 0.0 ± 0.0
Arg
5.266ArgAla: 5.266 ± 0.531
0.465ArgCys: 0.465 ± 0.188
3.872ArgAsp: 3.872 ± 0.447
3.33ArgGlu: 3.33 ± 0.507
2.556ArgPhe: 2.556 ± 0.441
4.027ArgGly: 4.027 ± 0.589
0.774ArgHis: 0.774 ± 0.267
3.02ArgIle: 3.02 ± 0.479
3.717ArgLys: 3.717 ± 0.594
6.428ArgLeu: 6.428 ± 0.918
1.394ArgMet: 1.394 ± 0.274
1.471ArgAsn: 1.471 ± 0.302
2.633ArgPro: 2.633 ± 0.43
2.013ArgGln: 2.013 ± 0.367
3.33ArgArg: 3.33 ± 0.546
3.02ArgSer: 3.02 ± 0.422
3.562ArgThr: 3.562 ± 0.686
5.111ArgVal: 5.111 ± 0.735
1.239ArgTrp: 1.239 ± 0.38
1.471ArgTyr: 1.471 ± 0.324
0.0ArgXaa: 0.0 ± 0.0
Ser
5.808SerAla: 5.808 ± 0.611
0.232SerCys: 0.232 ± 0.145
3.407SerAsp: 3.407 ± 0.424
2.788SerGlu: 2.788 ± 0.451
2.478SerPhe: 2.478 ± 0.414
5.189SerGly: 5.189 ± 0.718
0.697SerHis: 0.697 ± 0.197
3.098SerIle: 3.098 ± 0.604
3.175SerLys: 3.175 ± 0.451
3.872SerLeu: 3.872 ± 0.553
2.401SerMet: 2.401 ± 0.573
1.471SerAsn: 1.471 ± 0.234
2.71SerPro: 2.71 ± 0.5
2.478SerGln: 2.478 ± 0.447
3.717SerArg: 3.717 ± 0.475
4.104SerSer: 4.104 ± 0.46
3.872SerThr: 3.872 ± 0.512
4.027SerVal: 4.027 ± 0.549
1.162SerTrp: 1.162 ± 0.288
1.936SerTyr: 1.936 ± 0.412
0.0SerXaa: 0.0 ± 0.0
Thr
7.125ThrAla: 7.125 ± 0.904
0.155ThrCys: 0.155 ± 0.106
3.95ThrAsp: 3.95 ± 0.542
3.485ThrGlu: 3.485 ± 0.662
3.098ThrPhe: 3.098 ± 0.414
6.04ThrGly: 6.04 ± 0.656
1.084ThrHis: 1.084 ± 0.284
2.401ThrIle: 2.401 ± 0.447
3.253ThrLys: 3.253 ± 0.54
5.808ThrLeu: 5.808 ± 0.781
0.929ThrMet: 0.929 ± 0.253
1.549ThrAsn: 1.549 ± 0.313
4.104ThrPro: 4.104 ± 0.602
1.859ThrGln: 1.859 ± 0.357
4.724ThrArg: 4.724 ± 0.68
4.182ThrSer: 4.182 ± 0.634
3.407ThrThr: 3.407 ± 0.481
6.118ThrVal: 6.118 ± 0.769
1.859ThrTrp: 1.859 ± 0.442
2.013ThrTyr: 2.013 ± 0.378
0.0ThrXaa: 0.0 ± 0.0
Val
8.983ValAla: 8.983 ± 0.918
0.31ValCys: 0.31 ± 0.155
5.111ValAsp: 5.111 ± 0.715
4.646ValGlu: 4.646 ± 0.67
2.478ValPhe: 2.478 ± 0.422
4.646ValGly: 4.646 ± 0.574
1.317ValHis: 1.317 ± 0.332
3.795ValIle: 3.795 ± 0.56
3.175ValLys: 3.175 ± 0.465
6.97ValLeu: 6.97 ± 0.713
1.781ValMet: 1.781 ± 0.507
2.323ValAsn: 2.323 ± 0.419
3.562ValPro: 3.562 ± 0.515
3.407ValGln: 3.407 ± 0.543
4.569ValArg: 4.569 ± 0.612
4.259ValSer: 4.259 ± 0.443
5.886ValThr: 5.886 ± 0.63
5.189ValVal: 5.189 ± 0.766
1.781ValTrp: 1.781 ± 0.386
2.091ValTyr: 2.091 ± 0.325
0.0ValXaa: 0.0 ± 0.0
Trp
1.626TrpAla: 1.626 ± 0.358
0.155TrpCys: 0.155 ± 0.097
1.626TrpAsp: 1.626 ± 0.302
1.084TrpGlu: 1.084 ± 0.314
0.774TrpPhe: 0.774 ± 0.236
2.091TrpGly: 2.091 ± 0.359
0.852TrpHis: 0.852 ± 0.269
1.394TrpIle: 1.394 ± 0.357
0.697TrpLys: 0.697 ± 0.196
1.471TrpLeu: 1.471 ± 0.285
0.232TrpMet: 0.232 ± 0.118
0.62TrpAsn: 0.62 ± 0.19
0.929TrpPro: 0.929 ± 0.34
0.929TrpGln: 0.929 ± 0.326
1.007TrpArg: 1.007 ± 0.285
0.929TrpSer: 0.929 ± 0.271
1.471TrpThr: 1.471 ± 0.292
1.317TrpVal: 1.317 ± 0.349
0.774TrpTrp: 0.774 ± 0.322
0.852TrpTyr: 0.852 ± 0.266
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.323TyrAla: 2.323 ± 0.36
0.542TyrCys: 0.542 ± 0.196
2.013TyrAsp: 2.013 ± 0.314
2.865TyrGlu: 2.865 ± 0.565
0.62TyrPhe: 0.62 ± 0.175
2.71TyrGly: 2.71 ± 0.506
0.31TyrHis: 0.31 ± 0.194
1.317TyrIle: 1.317 ± 0.244
0.387TyrLys: 0.387 ± 0.171
1.859TyrLeu: 1.859 ± 0.348
0.62TyrMet: 0.62 ± 0.263
1.084TyrAsn: 1.084 ± 0.317
1.084TyrPro: 1.084 ± 0.305
1.317TyrGln: 1.317 ± 0.371
2.013TyrArg: 2.013 ± 0.494
1.239TyrSer: 1.239 ± 0.263
1.471TyrThr: 1.471 ± 0.323
1.781TyrVal: 1.781 ± 0.403
0.774TyrTrp: 0.774 ± 0.212
0.852TyrTyr: 0.852 ± 0.314
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (12914 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski